FastQCFastQC Report
Wed 25 May 2016
SRR1779998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357877
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4313912.05414150671879No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG97002.7104284432919687No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG14510.4054465640429533No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT10700.29898540560024817No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC10370.2897643603808012Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT150.002224265469.9951157
AAGACCG252.3896301E-455.9960825
CGCTGTA252.3896301E-455.99608226
GGTCGAC200.00694622652.50366647
AAACGCG200.00694622652.50366652
ACGCGAA200.006950066452.4963354
GGACCTA200.006950066452.496331
GCCGTAT15450.049.60818554
TCGCCGT15200.049.5100452
CCGTATC15700.049.0411655
CGCCGTA15650.048.757453
CGTATCA15750.048.44106356
TCGGTGG15600.048.2405545
CGGTGGT15650.048.0864346
TGGTCGC15600.048.01617449
GTCGCCG15700.047.9332951
TCTCGGT15650.047.8627743
CTCGGTG15650.047.6391144
GTATCAT16150.047.02458257
GATCTCG15950.046.96253241
Produced by FastQC (version 0.11.5)