FastQCFastQC Report
Wed 25 May 2016
SRR1779998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357877
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT198895.557496011199379Illumina PCR Primer Index 1 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163544.569726470267717No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT66241.8509152585944333Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG36061.0076087594341072No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC21820.6097066869343377No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT10740.3001031080510902Illumina PCR Primer Index 1 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC6490.18134722264912245No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC6330.17687641284575428No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT5400.15088983086367663Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG150.002219354770.0341232
ACGTATG301.0237636E-769.9851940
TGCCGAC501.4551915E-1162.9866745
AGGGGGG35600.062.0233970
AAACGAT401.0602889E-861.2798532
ATTATAC10350.057.1456531
GCCGACT509.804353E-1055.98815546
TTCACGA200.0069424452.51090222
TCACGGT200.006946278752.50356360
CGTGTAA200.006946278752.50356361
TTGTGCT200.00695011952.4962274
TGTACAA200.00695011952.4962275
AACGTAT200.006953961752.4888939
CACGTAT200.006953961752.4888939
CCGACTT552.2828317E-950.89832347
CCGTCTT43700.050.7672947
TATGCCG45000.050.6226243
CGTCTTC44300.050.55363548
ATGCCGT44800.050.53618244
GCCGTCT44100.050.46550846
Produced by FastQC (version 0.11.5)