FastQCFastQC Report
Wed 25 May 2016
SRR1779997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317919
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3865212.157813782755985No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG105403.315309874527789No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG14820.4661564738188029No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT11900.3743091793821697No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC11440.35984008505311105Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG3400.10694547982347705No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT150.0022234270.000015
TACGATC150.0022234270.0000114
ACGAGTT150.0022234270.0000150
AATCGCG150.0022234270.0000156
TTTCGTC200.006947434552.518
CGTCGAC200.006947434552.561
GAACCGG200.006947434552.570
GACGAGT200.006947434552.549
TCGCGAC200.006947434552.511
ACGGTCC200.006947434552.522
ACGCGTC200.006947434552.56
TGCGCGT305.8729516E-446.6666685
CTTTATA1000.045.4999962
GCTCTAC551.3085992E-744.5454521
TCGGTGG17600.044.54545245
GTCGCCG17550.044.47293551
TCGCCGT17500.044.452
GCCGTAT17500.044.454
CTCGGTG17650.044.22096344
CGGTGGT17650.044.22096346
Produced by FastQC (version 0.11.5)