FastQCFastQC Report
Wed 25 May 2016
SRR1779997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317919
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT196356.1761014598058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176395.548268584136211No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT56721.7841022398787112No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG30610.962823863940186No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC26230.8250529222852361No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT8760.2755418833098997TruSeq Adapter, Index 25 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT6120.1925018636822587No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC5560.17488731406427424No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC5530.1739436774775965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG33900.060.8088970
GCCGTAT352.969864E-759.99770746
AAGCCGT553.6379788E-1157.2705444
ATTATAC10650.054.8891641
TCACGAT200.006944163752.5062530
CGACCCA200.006948485552.49799317
GTTACAC200.006948485552.49799360
AATCTCG407.4690433E-752.49799336
CTCGAAA200.006948485552.49799339
AAGGGGG39250.052.43111869
CCGTCTT38100.052.2683547
GCCGTCT38350.052.20139746
CGTCTTC38500.052.0889248
TATGCCG39900.051.66469243
CGTATGC40600.051.54975541
ATGCCGT39950.051.3372144
GTATGCC40350.051.26198242
TGCCGTC39600.051.26066645
TCGTATG41250.051.16168240
CTTTATA1300.051.1599432