FastQCFastQC Report
Wed 25 May 2016
SRR1779996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157025
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4047425.77551345327177No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG109306.960675051743354No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT13700.8724725362203472No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG13640.8686514886164624No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC10830.6896990925011941Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG3180.2025155230058908No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG2090.1330998248686515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGC150.002221318470.026
CCGCTAT150.002221318470.032
TCCAGAC252.3846616E-456.0000043
GTCCAGA252.3846616E-456.0000042
TTTAGAG200.00694091752.50000453
AGACTAT200.00694091752.50000444
GGTGTCG200.00694091752.50000441
GCTGCGC200.00694091752.50000426
CCATGAT200.00694091752.50000416
GCCGCTA200.00694091752.50000431
GTTACCC200.00694091752.50000470
CGCTATG200.00694091752.50000433
CTGCCGG200.00694091752.50000418
TCATGTA200.00694091752.50000431
CGGTGCG200.00694091752.50000428
GGTGCGT200.00694091752.5000046
CGTCGGC200.00694091752.50000461
TTTGAAC200.00694091752.50000431
CACAACG200.00694091752.50000438
TGTCGGT352.0791704E-550.00000443
Produced by FastQC (version 0.11.5)