FastQCFastQC Report
Wed 25 May 2016
SRR1779996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157025
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1848611.772647667568858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1806411.5039006527623No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT79425.057793345008756No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG35382.253144403757364No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC26371.6793504219073396No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC8550.5444992835535744No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT7380.469988855277822Illumina PCR Primer Index 6 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT6120.38974685559624267No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC5090.32415220506288805No Hit
ATTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3960.2521891418563923No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1890.12036299952236905No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1850.11781563445311258No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA150.00222159969.997777
GACAAGG207.908595E-569.997772
GCCGGGA150.00222159969.9977757
CGTCTTA150.00222159969.9977748
CGATTAG150.00222159969.9977719
ACGATAC353.6870915E-969.9977727
CGATACA353.6870915E-969.9977728
ATTATAC10500.065.664571
AGGGGGG31500.064.2201870
ACGATCA600.064.1646327
AGCCGTC601.8189894E-1258.33147445
CCGACTT252.3850375E-455.99821547
CGACTTC252.3850375E-455.99821548
GAGGGTA252.3850375E-455.99821536
AAGCCGT700.054.9982544
CGCTCCA452.6842827E-854.4427128
AAGGGGG37650.053.7300369
ACACGTT200.006941792652.4983350
TTAATGC200.006941792652.4983356
CAACGAT200.006941792652.4983348
Produced by FastQC (version 0.11.5)