FastQCFastQC Report
Wed 25 May 2016
SRR1779995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420140
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380579.058171085828533No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG91192.170466987194745No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG13190.3139429713904889No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC11240.2675298709953825Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT10500.2499166944351883No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTC150.002224084569.998693
GTCCGCG150.002224084569.9986921
CGCTCAA200.00694949752.49901646
ACGAGAT200.00694949752.49901668
TCGGTTA200.00694949752.49901626
GGTACGG305.875664E-446.6657941
GTCGCCG15800.045.18902651
GCCGTAT15500.044.93464354
CGGTGGT15950.044.76405346
TGGTCGC15850.044.60484749
TCGGTGG15950.044.5446245
CTCGGTG15900.044.46457744
TCGCCGT15600.044.4222552
GTAGATC16400.044.38941638
GTATCAT16250.043.50687857
CGCCGTA16100.043.47744853
GATCTCG16350.043.45484541
CGTATCA16300.043.3734256
ATCTCGG16450.043.19068542
TAGATCT16550.043.14118639
Produced by FastQC (version 0.11.5)