FastQCFastQC Report
Wed 25 May 2016
SRR1779995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420140
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT173514.12981387156662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157233.7423239872423477No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT53681.2776693483124673No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG26030.6195553862998049No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC21130.5029275955633836No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC5450.1297186652068358No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC5360.12757652211167705No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT4680.11139144094825534Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA150.002224278669.9971545
TCGAAAG207.924372E-569.9971540
AGGGGGG28600.060.819270
CTCGAAA252.3897676E-455.99771539
AAGCCGT1050.053.33115444
GACGTAT200.00695010352.49785646
CCTCGAA200.00695010352.4978569
TTGAGCG200.00695010352.49785633
TGCGATA200.00695010352.4978569
ACGTATG800.052.49785640
CGACTAT200.00695010352.49785646
GACGAAT200.00695010352.49785646
CGTCCCG200.00695010352.49785651
TGTCGGT200.00695010352.49785616
GCATCGA200.00695010352.49785632
CTAGCGT200.00695010352.49785614
GCCGTCT29700.050.90701746
TATGCCG35200.050.90701343
ATATCGT552.2810127E-950.90701337
CGTCTTC27800.050.86123748