FastQCFastQC Report
Wed 25 May 2016
SRR1779994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67017
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3649654.45782413417491No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG39735.928346538937881No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG4980.7430950355879851No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT4510.6729635764059865No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC4180.6237223391079875No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1300.1939806317799961No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG840.1253413313039975No Hit
CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG810.12086485518599757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAG150.002216401769.9947834
GAGCTTA150.002216401769.994781
GCAGCGG150.002216401769.994788
CTCAGGG150.002216401769.9947846
AGCGCAC150.002216401769.9947810
TCTCAGG150.002216401769.9947845
CGCGATC150.002216401769.9947844
GCGGGTG150.002216401769.994786
CCTATTA200.00690524552.5352927
CTTTTAT200.00692568452.49608654
AGGGGCT200.00692568452.49608654
TTGTTAT200.00692568452.49608654
AATTAAA200.00692568452.49608643
ACTCTAT200.00692568452.49608637
CTCTATC200.00692568452.49608638
AACTCTT200.00692568452.49608650
TAACTCT200.00692568452.49608649
AGAAATA200.00692568452.4960866
TTCTCAG200.00692568452.49608644
CAAAGTA200.00692568452.49608644
Produced by FastQC (version 0.11.5)