FastQCFastQC Report
Wed 25 May 2016
SRR1779994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67017
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2095231.263709208111372No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64789.666204097467807No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC30454.543623259769909No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG27684.130295298207917No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT13982.086037870987958No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT7401.1041974424399779TruSeq Adapter, Index 11 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT6470.9654266827819807No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC3920.5849262127519883No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC3050.4551084053299909No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT3020.45063192921199097No Hit
CTAAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2640.3939298983839921No Hit
TCATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG2140.3193219630839936No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT1470.21934732978199561No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1470.21934732978199561No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT1390.20741006013399585No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1280.1909963143679962No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT1170.1745825686019965RNA PCR Primer, Index 11 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC1090.16264529895399676No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC1010.15070802930599697No Hit
CAAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT880.1313099661279974No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC850.12683349000999747No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT810.12086485518599757No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC790.11788053777399765No Hit
CATAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT740.1104197442439978No Hit
ATACCCATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT730.10892758553799782TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT700.10445110941999791No Hit
CTTATACACATCTCCGAGCCAACGAGACTCAGCTATATCTCGTATGCCGT690.10295895071399794No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCA680.10146679200799798No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGTT150.002210309570.0433230
GAGTTAC150.002210309570.0433232
TCTGATA150.002210309570.0433222
CCGATAA150.002210309570.0433222
GATATAT207.8502046E-570.0433132
CTCAGCA401.3096724E-1070.0433128
AGACTAA207.8502046E-570.0433125
GAGACTA207.8502046E-570.0433124
CGTTTCC150.002216870769.9910518
TGCGCTT150.002216870769.9910546
GACAGGC150.002216870769.991059
ATGACGA150.002216870769.9910544
ATGATAC150.002216870769.9910546
TAAGCCG301.0105214E-769.9910543
TGCGATC150.002216870769.991058
GACGGCT150.002216870769.9910556
GCGCGTT150.002216870769.9910515
GATCGCG150.002216870769.9910511
GTTTCCA150.002216870769.9910519
ATGCGAT150.002216870769.991057
Produced by FastQC (version 0.11.5)