FastQCFastQC Report
Wed 25 May 2016
SRR1779993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55965
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3295158.87787009738229No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG29725.31046189582775No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3560.6361118556240507No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3540.6325381935138032No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3080.5503439649781113No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1060.18940409184311624No Hit
CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG670.11971768069329045No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGG150.002213828570.04
GTGTTCA207.862984E-570.01
CTTTATA207.862984E-570.02
GATATTC150.002213828570.015
ACATGCC150.002213828570.050
GCGACCC150.002213828570.028
TGTTCAC207.862984E-570.02
GGACATG150.002213828570.048
GGATATT150.002213828570.014
TCACGGT150.002213828570.05
CGGGATA150.002213828570.012
ATGCCCG150.002213828570.052
CCCTGCC150.002213828570.033
CGCAGGA150.002213828570.044
TATTCGC150.002213828570.017
GGGATAT150.002213828570.013
GACCCCT150.002213828570.030
CGGTCGG150.002213828570.08
TCTTTAT207.862984E-570.01
ACGGTCG150.002213828570.07
Produced by FastQC (version 0.11.5)