FastQCFastQC Report
Wed 25 May 2016
SRR1779993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55965
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT1951534.87000804073975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51159.13964084695792No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG26754.779773072455999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC25034.472438130974717No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT12392.2138836772983113No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT6551.1703743411060485TruSeq Adapter, Index 9 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT5861.0470829983025105No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC4340.7754846779237023No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT3520.6289645314035558No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC2620.46814973644241936No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2440.4359867774501921No Hit
TCATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG2120.3788081836862325No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG1190.21263289555972484No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC1160.20727240239435363No Hit
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT1130.2019119092289824No Hit
CAAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT970.17332261234700258No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC920.1643884570713839No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC830.14830697757527025No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGACGT800.14294648440989904No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC780.13937282229965156No Hit
CATAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT730.13043866702403287No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT730.13043866702403287No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT710.1268650049137854No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG660.11793084963816672No Hit
ATACCCATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT610.10899669436254802TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAGT600.10720986330742428No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTCGGATCTCGTATGCCGT590.10542303225230054No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATATCGTATGCCGT580.10363620119717681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAAGT252.805802E-670.0000124
TATACGG252.805802E-670.0000130
TACGGAT252.805802E-670.0000132
GTATACG252.805802E-670.0000129
ATACGGA353.6288839E-970.0000131
CGACTTC252.805802E-670.0000148
ACAAATC207.862984E-570.06
CGACACC150.002213828570.039
ACTCGTA150.002213828570.038
AATCTCC207.862984E-570.09
AGAAGTA301.0065742E-770.025
CTTTACA150.002213828570.01
ATTAGGA150.002213828570.031
GTCAAAC150.002213828570.033
CACAACT150.002213828570.07
GAAGTCA150.002213828570.030
ACTCCGA150.002213828570.011
TCGGAAC301.0065742E-770.033
TCAGCAT150.002213828570.053
CGGAACT207.862984E-570.034
Produced by FastQC (version 0.11.5)