##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779993_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55965 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.51262396140445 32.0 27.0 32.0 27.0 32.0 2 30.26019833824712 32.0 32.0 32.0 27.0 32.0 3 28.488001429464845 32.0 27.0 32.0 13.0 32.0 4 31.035718752791922 32.0 32.0 32.0 27.0 32.0 5 28.601465201465203 32.0 27.0 32.0 22.0 32.0 6 34.9480032162959 37.0 37.0 37.0 32.0 37.0 7 34.56658625926919 37.0 37.0 37.0 27.0 37.0 8 34.192852675779505 37.0 32.0 37.0 27.0 37.0 9 34.08521397301885 37.0 37.0 37.0 27.0 37.0 10-11 35.06037702135263 37.0 37.0 37.0 29.5 37.0 12-13 35.03637094612704 37.0 37.0 37.0 32.0 37.0 14-15 35.00248369516662 37.0 37.0 37.0 27.0 37.0 16-17 33.99799874921826 37.0 34.5 37.0 27.0 37.0 18-19 34.35679442508711 37.0 37.0 37.0 27.0 37.0 20-21 34.873358348968104 37.0 37.0 37.0 29.5 37.0 22-23 34.60888055034397 37.0 37.0 37.0 27.0 37.0 24-25 34.949870454748506 37.0 37.0 37.0 29.5 37.0 26-27 35.20445814348253 37.0 37.0 37.0 32.0 37.0 28-29 35.06559456803359 37.0 37.0 37.0 32.0 37.0 30-31 35.08567854909318 37.0 37.0 37.0 32.0 37.0 32-33 34.89110158134548 37.0 37.0 37.0 32.0 37.0 34-35 34.46977575270258 37.0 37.0 37.0 27.0 37.0 36-37 34.04564459930314 37.0 37.0 37.0 27.0 37.0 38-39 33.374144554632366 37.0 32.0 37.0 22.0 37.0 40-41 34.01038148843027 37.0 34.5 37.0 24.5 37.0 42-43 34.58612525685696 37.0 37.0 37.0 27.0 37.0 44-45 34.716697936210124 37.0 37.0 37.0 27.0 37.0 46-47 34.32961672473868 37.0 37.0 37.0 27.0 37.0 48-49 33.31528633967658 37.0 34.5 37.0 22.0 37.0 50-51 33.869302242472976 37.0 37.0 37.0 24.5 37.0 52-53 33.56043062628429 37.0 34.5 37.0 22.0 37.0 54-55 33.577745019208436 37.0 34.5 37.0 22.0 37.0 56-57 33.457750379701594 37.0 34.5 37.0 22.0 37.0 58-59 33.561797552041455 37.0 34.5 37.0 22.0 37.0 60-61 33.70463682658804 37.0 37.0 37.0 24.5 37.0 62-63 34.10481550969356 37.0 37.0 37.0 27.0 37.0 64-65 34.1772804431341 37.0 37.0 37.0 27.0 37.0 66-67 33.945930492271955 37.0 37.0 37.0 27.0 37.0 68-69 33.3166353971232 37.0 32.0 37.0 22.0 37.0 70-71 30.980416331635844 37.0 29.5 37.0 13.0 37.0 72-73 32.78231930670955 37.0 34.5 37.0 17.5 37.0 74-75 34.03114446529081 37.0 37.0 37.0 24.5 37.0 76 34.5421781470562 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.3974475594739282 11101 2 -1.54702373165399 11101 3 -1.6678410829854613 11101 4 -0.6689374778575186 11101 5 -1.5161798499953925 11101 6 -2.5800754832226787 11101 7 -1.3182034765951869 11101 8 -2.085456905693146 11101 9 -2.315944462219562 11101 10-11 -1.4008724523019467 11101 12-13 -0.7002065409641034 11101 14-15 -3.3865063962085493 11101 16-17 -2.0981088221312447 11101 18-19 -0.7156768295791451 11101 20-21 -0.1530811030814192 11101 22-23 -0.4572376682038737 11101 24-25 -0.4164059116313581 11101 26-27 -0.80404916728871 11101 28-29 0.3520183860249446 11101 30-31 -0.7259362436115708 11101 32-33 1.3855179524871417 11101 34-35 -3.5485425890325644 11101 36-37 -1.1753573627046592 11101 38-39 -0.5423632453726199 11101 40-41 -0.20519015340227753 11101 42-43 -1.54591949455547 11101 44-45 0.33770988045176864 11101 46-47 -0.9717365025158671 11101 48-49 -1.0977399641044627 11101 50-51 -0.07617514240002521 11101 52-53 0.08252720850309458 11101 54-55 -1.9454746853968352 11101 56-57 -0.5928952482793903 11101 58-59 -1.4388611828786964 11101 60-61 -0.35387420787150603 11101 62-63 0.7298492638996379 11101 64-65 -2.1448279029647352 11101 66-67 -2.713325721194437 11101 68-69 -2.033356620638049 11101 70-71 -0.7290567237216834 11101 72-73 -3.2310039411767164 11101 74-75 -1.3511890638665065 11101 76 0.34280376822796654 11102 1 -1.5264030152387136 11102 2 -2.1303570649873222 11102 3 -2.561519243905 11102 4 -0.6991098916506218 11102 5 0.8358316442574818 11102 6 0.43141876965088244 11102 7 0.3082333050140136 11102 8 -0.34695115856671066 11102 9 0.24440036536664422 11102 10-11 -3.473429923566318 11102 12-13 -0.5435973455618068 11102 14-15 -0.8900983502315327 11102 16-17 0.588672787064155 11102 18-19 -0.9369411973952424 11102 20-21 -3.436845470897513 11102 22-23 -2.1404273233762865 11102 24-25 -1.4056300495623901 11102 26-27 -0.3270376730358393 11102 28-29 -0.5014298898371194 11102 30-31 -0.25179831257708685 11102 32-33 0.1836501363951939 11102 34-35 -0.016933393630260696 11102 36-37 -0.5704723052333946 11102 38-39 -2.130006923533543 11102 40-41 -1.3337821074252645 11102 42-43 -3.241321793406037 11102 44-45 -1.6342728781689217 11102 46-47 -1.736104318607822 11102 48-49 -4.5158434123803275 11102 50-51 -1.3046234182620964 11102 52-53 -1.2831337110371308 11102 54-55 -2.4181758348221223 11102 56-57 -3.3357113402334093 11102 58-59 -1.5430278495453607 11102 60-61 -0.2691040929289841 11102 62-63 -0.7270472878245045 11102 64-65 -1.3926727305509417 11102 66-67 -1.4906245717691533 11102 68-69 -0.8688451263851746 11102 70-71 -1.6019015513078898 11102 72-73 -4.7755441710617745 11102 74-75 -1.099752282257306 11102 76 -4.759207726024904 11103 1 1.2852680722944427 11103 2 -0.5534339880642456 11103 3 -1.2511744163187934 11103 4 0.3791394452194048 11103 5 -2.0898977987133414 11103 6 1.5096681065209054 11103 7 -1.8310239894156979 11103 8 -0.38673895697520067 11103 9 -0.10440600068110228 11103 10-11 -0.19093655486605599 11103 12-13 0.1235114077538384 11103 14-15 0.7657371935350383 11103 16-17 0.04291681889439758 11103 18-19 -1.0490101629124808 11103 20-21 0.1049317174314055 11103 22-23 -1.3466607451269539 11103 24-25 -0.4692905270159713 11103 26-27 0.5164636532241076 11103 28-29 -0.5325969985904422 11103 30-31 -2.1105516282269576 11103 32-33 -0.7891615346923402 11103 34-35 -0.9267477172376886 11103 36-37 -0.447793260140557 11103 38-39 -1.673773501782879 11103 40-41 -0.27410040981253303 11103 42-43 -2.4946374432734117 11103 44-45 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24-25 -0.6195309116313581 11104 26-27 -0.2155075006220457 11104 28-29 -0.8511066139750554 11104 30-31 -0.16343624361157083 11104 32-33 -0.4165653808461869 11104 34-35 -1.4808342556992287 11104 36-37 0.12672597062866942 11104 38-39 -1.4069465787059556 11104 40-41 -1.0541484867356132 11104 42-43 0.30824717211120145 11104 44-45 -0.7247901195482314 11104 46-47 -0.11756983584920278 11104 48-49 0.48038503589553727 11104 50-51 0.4081998575999748 11104 52-53 -0.625806124830234 11104 54-55 -1.190266352063503 11104 56-57 -0.7231035816127189 11104 58-59 0.7069721504546393 11104 60-61 -0.45283254120484173 11104 62-63 -1.051400736100362 11104 64-65 -0.5823279029647352 11104 66-67 -1.3279090545277725 11104 68-69 -0.929189953971381 11104 70-71 1.4584432762783166 11104 72-73 0.06066272548994789 11104 74-75 0.1383942694668292 11104 76 -2.1363628984386978 11105 1 -0.7831079960816254 11105 2 0.6474207127904563 11105 3 -0.5567299718743506 11105 4 0.2893958554758136 11105 5 0.5254868166712754 11105 6 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11105 66-67 0.02278538991667034 11105 68-69 -0.17918995397138104 11105 70-71 -0.027667834832794114 11105 72-73 -0.49489283006560925 11105 74-75 0.7460331583557149 11105 76 0.2455815460057451 11106 1 -0.6584467494691602 11106 2 -0.7401131625482975 11106 3 -1.470686611440744 11106 4 0.24874544897175 11106 5 -1.0842692808896999 11106 6 -0.5475551580194278 11106 7 0.3443981494210746 11106 8 -0.3191967430915241 11106 9 1.4706409036340986 11106 10-11 -0.6778033466108866 11106 12-13 0.5569072801741015 11106 14-15 0.17802612411665564 11106 16-17 -0.01477548879790902 11106 18-19 0.20098983708751916 11106 20-21 0.34742702699988115 11106 22-23 -0.48315230235022 11106 24-25 -0.3173205457776973 11106 26-27 0.7284711579145409 11106 28-29 0.8337257030981178 11106 30-31 0.13381985394940443 11106 32-33 -0.08043733206569925 11106 34-35 0.09576635405687028 11106 36-37 0.5329759706286694 11106 38-39 0.11515707983062384 11106 40-41 0.2831228547278002 11106 42-43 -0.17193575471807065 11106 44-45 0.2538684170371397 11106 46-47 0.8900521153703096 11106 48-49 -0.07639850068983378 11106 50-51 1.4573614429658264 11106 52-53 0.647059728828296 11106 54-55 1.1835903552535711 11106 56-57 1.25974702814338 11106 58-59 0.469167272405862 11106 60-61 0.13291441001467064 11106 62-63 -0.4713702482954858 11106 64-65 -0.2652547322330321 11106 66-67 -0.007024908186309631 11106 68-69 -0.9108972710445542 11106 70-71 1.7328335201807548 11106 72-73 2.0911505303679974 11106 74-75 1.2778759767839034 11106 76 1.234063930829592 11107 1 -0.2667681268005815 11107 2 -0.7185923591049708 11107 3 -1.2511744163187934 11107 4 0.3678272280248329 11107 5 -0.7686308303875471 11107 6 -1.2075264636148404 11107 7 0.7798357390910908 11107 8 1.4096411335225412 11107 9 -1.2130032857489752 11107 10-11 -1.2893528444588185 11107 12-13 0.4787150276633412 11107 14-15 -0.4514573766007075 11107 16-17 -0.853010782915554 11107 18-19 -0.1225395746771838 11107 20-21 -0.5195026717088709 11107 22-23 -0.46091413879210563 11107 24-25 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1.557352737594627 11109 46-47 0.9377873070079445 11109 48-49 0.9486886073241152 11109 50-51 0.7845391433142623 11109 52-53 1.061693875169766 11109 54-55 0.927144362222208 11109 56-57 0.2987714183872825 11109 58-59 0.9426864361689269 11109 60-61 0.16636388736658603 11109 62-63 0.6655635496139212 11109 64-65 1.5033863827495537 11109 66-67 1.7354838026150858 11109 68-69 0.7779529031714745 11109 70-71 1.8941575619925999 11109 72-73 1.5963770112042326 11109 74-75 0.9642871266096904 11109 76 0.4654228158470133 11110 1 -0.37906759204122054 11110 2 -0.7667207013509589 11110 3 -0.6451138102581879 11110 4 0.6530322191121769 11110 5 -1.050270759086299 11110 6 -0.8831057862529832 11110 7 -0.6060822644739758 11110 8 -0.8506084208446651 11110 9 -0.005338401613499855 11110 10-11 -0.43685730078679796 11110 12-13 -0.21914593490350143 11110 14-15 1.1987208765187276 11110 16-17 0.07613360211118447 11110 18-19 0.36765650375419057 11110 20-21 -0.10194473944505944 11110 22-23 0.3135956651294549 11110 24-25 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21611 44-45 0.08313670972006548 21611 46-47 0.4388326031751859 21611 48-49 1.5089673529687104 21611 50-51 0.32321510150241295 21611 52-53 1.354376801999038 21611 54-55 0.7201757211072248 21611 56-57 0.4914543452165532 21611 58-59 0.761850199235127 21611 60-61 0.681694897819547 21611 62-63 -0.3006385409784116 21611 64-65 -0.6676937566232652 21611 66-67 0.9319994820575985 21611 68-69 0.8573954118822726 21611 70-71 0.7938091299368537 21611 72-73 0.07895540841677473 21611 74-75 2.265680854832681 21611 76 0.7706492966832528 21612 1 0.30397909100545917 21612 2 0.7074807728505164 21612 3 0.4785553134109364 21612 4 0.9560625221424814 21612 5 0.9038651950496543 21612 6 -0.5350304381776354 21612 7 1.4610758026840927 21612 8 -0.8399614101976525 21612 9 -1.4172958135709166 21612 10-11 0.8620779981485001 21612 12-13 0.14326192750435496 21612 14-15 0.3223900001878448 21612 16-17 0.8636028895804699 21612 18-19 1.6334222695199543 21612 20-21 0.025409887909567885 21612 22-23 1.2775596290934246 21612 24-25 1.5379859802605296 21612 26-27 -0.23197709521664223 21612 28-29 0.5209373049438639 21612 30-31 0.5781178104424782 21612 32-33 0.371441375910571 21612 34-35 0.48073669024671517 21612 36-37 0.9789219165746204 21612 38-39 1.1006547726453988 21612 40-41 0.7570846213724991 21612 42-43 0.9720985234625559 21612 44-45 0.4542639345058248 21612 46-47 1.1679031371237656 21612 48-49 2.10158436021986 21612 50-51 -0.7282021694270568 21612 52-53 -0.816684503208613 21612 54-55 0.26961540469325485 21612 56-57 -0.990417770801912 21612 58-59 -0.6241089306264413 21612 60-61 0.5459005669032706 21612 62-63 0.8041735882239607 21612 64-65 -1.6769224975593318 21612 66-67 0.6323949995262765 21612 68-69 0.9018911271096997 21612 70-71 0.7354703033053411 21612 72-73 0.621473536300762 21612 74-75 0.9720091343316923 21612 76 0.443028993453197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 4.0 18 3.0 19 14.0 20 46.0 21 94.0 22 184.0 23 312.0 24 414.0 25 531.0 26 731.0 27 899.0 28 1185.0 29 1375.0 30 1949.0 31 2581.0 32 3668.0 33 5979.0 34 9905.0 35 15832.0 36 10258.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.933743120577514 6.8740618969337435 9.965334858123079 61.226860124365665 2 16.446874664951217 9.368857438976448 58.68803831171152 15.496229584360815 3 16.943034808090914 14.650489600457437 54.03116289042956 14.375312701022086 4 14.337532386312873 51.81631376753329 22.124542124542124 11.72161172161172 5 14.832484588582147 21.40087554721701 56.640757616367374 7.125882247833467 6 18.3806579582209 62.05750433337502 11.80643662550705 7.755401082897018 7 18.048780487804876 17.65746448673278 11.691235593674618 52.60251943178772 8 16.683641561690344 62.49798981506298 6.804252657911195 14.014115965335478 9 16.449566693469134 13.746091307066916 5.383721969087823 64.42062003037613 10-11 17.719268464830385 36.457933154053016 30.263827962369 15.5589704187476 12-13 17.866523720182258 9.902617707495756 36.41204324131153 35.81881533101045 14-15 18.585723219869564 7.1562583757705704 12.413115339944609 61.844903064415256 16-17 44.366121683194855 30.28142589118199 5.632091485750022 19.720360939873135 18-19 47.35817028499955 14.051639417493075 5.782185294380417 32.80800500312696 20-21 21.51612615027249 7.217010631644778 5.731260609309389 65.53560260877333 22-23 18.678805314083036 34.21901383913016 5.380196374487398 41.721984472299404 24-25 44.42161128633078 34.27266960320935 5.596704877460397 15.709014232999474 26-27 48.445234733813464 36.71479886341298 6.815054416784316 8.024911985989242 28-29 44.28688861487875 13.991493772226093 7.383084043674833 34.33853356922032 30-31 23.89495974767466 30.286189118916358 33.97546439835953 11.843386735049455 32-33 18.646581608948928 13.671777277438263 59.3509881705443 8.330652943068511 34-35 40.967246216250025 7.622893697621643 17.423118846380646 33.986741239747694 36-37 50.148306977575274 29.561332975967126 7.845975163048334 12.444384883409274 38-39 20.905029929420174 13.02599839185205 33.73179665862593 32.33717502010185 40-41 17.011391556846103 6.795622068349341 37.89591244136699 38.29707393343757 42-43 43.803048442721085 5.926236978003323 35.32959276664939 14.941121812626198 44-45 20.049137854015903 32.41400875547217 38.11668006789958 9.420173322612348 46-47 42.82510609783337 9.811927630109448 15.569354478445389 31.79361179361179 48-49 42.45510586974002 6.671133744304476 9.13428035379255 41.73948003216296 50-51 21.478602698114894 7.1571517912981335 35.32118288215849 36.04306262842848 52-53 15.47217010631645 7.484141874385777 61.551862771374964 15.491825247922808 54-55 14.938308004324247 7.2270319047236145 42.16051533588857 35.67414475506357 56-57 41.96730099169124 6.339676583579022 15.6982042347896 35.99481818994014 58-59 19.289358242425866 8.977279834177633 56.222359216274896 15.511002707121607 60-61 41.78951130170642 32.78388278388278 16.469221835075494 8.957384079335299 62-63 18.662556955239882 66.20566425444474 5.9957116054677035 9.136067184847672 64-65 14.887780994246095 70.06450805904007 5.853078874950859 9.194632071762982 66-67 15.382484856065615 70.33129031681646 5.525972517556243 8.760252309561675 68-69 17.422496202984007 68.60180469936567 5.180916644331279 8.794782453319039 70-71 40.955954614491205 45.20771911015814 5.505226480836237 8.331099794514428 72-73 75.96447652913531 9.98606221968086 6.0003931168807965 8.04906813430302 74-75 79.20910617919303 6.205996926485829 6.423108537936457 8.16178835638469 76 79.97355301013206 6.007755401082897 6.179303443469559 7.839388145315487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 3.0 12 6.0 13 4.0 14 1.0 15 1.5 16 2.0 17 1.0 18 6.5 19 20.5 20 31.5 21 34.0 22 25.5 23 31.0 24 42.5 25 40.0 26 46.5 27 80.5 28 97.5 29 87.0 30 96.0 31 112.5 32 126.5 33 133.0 34 159.5 35 198.5 36 197.5 37 184.0 38 218.0 39 261.5 40 332.5 41 464.5 42 535.0 43 614.0 44 851.5 45 1595.0 46 2180.0 47 5196.5 48 15772.0 49 12342.0 50 1353.0 51 1320.0 52 974.0 53 655.5 54 650.0 55 681.5 56 748.0 57 833.5 58 884.0 59 843.0 60 795.5 61 736.0 62 683.0 63 687.0 64 649.5 65 550.0 66 454.5 67 417.0 68 372.0 69 319.5 70 293.5 71 275.0 72 236.5 73 175.0 74 133.0 75 114.0 76 106.0 77 82.0 78 52.5 79 39.0 80 34.0 81 21.0 82 9.0 83 5.0 84 9.0 85 9.5 86 4.5 87 3.0 88 3.5 89 4.5 90 4.0 91 3.0 92 3.0 93 3.0 94 5.5 95 13.0 96 18.0 97 26.5 98 86.0 99 2583.5 100 5030.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001786831055123738 2 0.005360493165371214 3 0.001786831055123738 4 0.0 5 0.0 6 0.007147324220494952 7 0.0 8 0.0 9 0.0 10-11 8.93415527561869E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.93415527561869E-4 24-25 0.006253908692933083 26-27 0.014294648440989904 28-29 0.010720986330742428 30-31 0.00982757080318056 32-33 0.005360493165371214 34-35 0.003573662110247476 36-37 0.0 38-39 0.0 40-41 0.004467077637809345 42-43 0.003573662110247476 44-45 0.0 46-47 0.004467077637809345 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.002680246582685607 56-57 0.0 58-59 0.002680246582685607 60-61 0.0 62-63 0.0 64-65 0.005360493165371214 66-67 0.003573662110247476 68-69 0.0 70-71 0.0 72-73 0.003573662110247476 74-75 0.005360493165371214 76 0.007147324220494952 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55965.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.941570624497455 #Duplication Level Percentage of deduplicated Percentage of total 1 68.18046011353451 12.232645403377111 2 9.112638183447864 3.2699008308764403 3 6.363907977293098 3.425355132672206 4 5.0194203764565275 3.602251407129456 5 3.9139527935464593 3.511123023318145 6 2.5595060252962853 2.755293487000804 7 1.693058460312718 2.1263289555972484 8 0.9461209042924011 1.357991601894041 9 0.4879992032666069 0.7879924953095686 >10 1.4241609401454038 4.040025015634772 >50 0.14938751120406335 1.8886804252657912 >100 0.07967333930883377 3.3092111140891634 >500 0.019918334827208443 2.217457339408559 >1k 0.029877502240812665 11.466094880729027 >5k 0.009959167413604222 9.13964084695792 >10k+ 0.009959167413604222 34.87000804073975 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT 19515 34.87000804073975 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5115 9.13964084695792 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG 2675 4.779773072455999 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC 2503 4.472438130974717 No Hit CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT 1239 2.2138836772983113 No Hit ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT 655 1.1703743411060485 TruSeq Adapter, Index 9 (95% over 21bp) CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT 586 1.0470829983025105 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC 434 0.7754846779237023 No Hit CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT 352 0.6289645314035558 No Hit CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC 262 0.46814973644241936 No Hit CTAAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT 244 0.4359867774501921 No Hit TCATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG 212 0.3788081836862325 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG 119 0.21263289555972484 No Hit TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC 116 0.20727240239435363 No Hit TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT 113 0.2019119092289824 No Hit CAAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT 97 0.17332261234700258 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC 92 0.1643884570713839 No Hit CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC 83 0.14830697757527025 No Hit CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGACGT 80 0.14294648440989904 No Hit CTCTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC 78 0.13937282229965156 No Hit CATAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT 73 0.13043866702403287 No Hit CTTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT 73 0.13043866702403287 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT 71 0.1268650049137854 No Hit CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG 66 0.11793084963816672 No Hit ATACCCATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT 61 0.10899669436254802 TruSeq Adapter, Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAGT 60 0.10720986330742428 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTCGGATCTCGTATGCCGT 59 0.10542303225230054 No Hit CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATATCGTATGCCGT 58 0.10363620119717681 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005360493165371214 0.0 2 0.0 0.0 0.0 0.005360493165371214 0.0 3 0.0 0.0 0.0 0.005360493165371214 0.0 4 0.0 0.0 0.0 0.01965514160636112 0.0 5 0.0 0.0 0.0 0.03931028321272224 0.0 6 0.0 0.0 0.0 0.03931028321272224 0.0 7 0.0 0.0 0.0 0.03931028321272224 0.0 8 0.0 0.0 0.0 0.03931028321272224 0.0 9 0.0 0.0 0.0 0.050031269543464665 0.0 10 0.0 0.0 0.0 0.08219422853569196 0.0 11 0.0 0.0 0.0 0.09291521486643438 0.0 12 0.0 0.0 0.0 0.10899669436254802 0.0 13 0.0 0.0 0.0 0.10899669436254802 0.0 14 0.0 0.0 0.0 0.13758599124452783 0.0 15 0.0 0.0 0.0 0.150093808630394 0.0 16 0.0 0.0 0.0 0.150093808630394 0.0 17 0.0 0.0 0.0 0.150093808630394 0.0 18 0.0 0.0 0.0 0.16796211918163137 0.0 19 0.0 0.0 0.0 0.22692754400071474 0.0 20 0.0 0.0 0.0 0.2394353613865809 0.0 21 0.0 0.0 0.0 0.24658268560707586 0.0 22 0.0 0.0 0.0 0.28231930670955063 0.0 23 0.0 0.0 0.0 0.31984275886714914 0.0 24 0.0 0.0 0.0 0.33592423836326274 0.0 25 0.0 0.0 0.0 0.3663003663003663 0.0 26 0.0 0.0 0.0 0.3877423389618512 0.0 27 0.0 0.0 0.0 0.416331635843831 0.0 28 0.0 0.0 0.0 0.491378540159028 0.0 29 0.0 0.0 0.0 0.5396229786473689 0.0 30 0.0 0.0 0.0 0.6307513624586796 0.0 31 0.0 0.0 0.0 0.6789958009470205 0.0 32 0.0 0.0 0.0 0.7183060841597427 0.0 33 0.0 0.0 0.0 0.8076476369159296 0.0 34 0.0 0.0 0.0 0.9523809523809523 0.0 35 0.0 0.0 0.0 1.206110962208523 0.0 36 0.0 0.0 0.0 1.624229429107478 0.0 37 0.0 0.0 0.0 2.147770928258733 0.0 38 0.0 0.0 0.0 2.6212811578665236 0.0 39 0.0 0.0 0.0 3.3324399178057713 0.0 40 0.0 0.0 0.0 4.004288394532297 0.0 41 0.0 0.0 0.0 4.47422496202984 0.0 42 0.0 0.0 0.0 5.028142589118199 0.0 43 0.0 0.0 0.0 5.562405074600196 0.0 44 0.0 0.0 0.0 6.021620655766998 0.0 45 0.0 0.0 0.0 6.47726257482355 0.0 46 0.0 0.0 0.0 7.034753864022156 0.0 47 0.0 0.0 0.0 7.472527472527473 0.0 48 0.0 0.0 0.0 7.87277762887519 0.0 49 0.0 0.0 0.0 8.26945412311266 0.0 50 0.0 0.0 0.0 8.646475475743769 0.0 51 0.0 0.0 0.0 8.987760207272402 0.0 52 0.0 0.0 0.0 9.304029304029305 0.0 53 0.0 0.0 0.0 9.584561779683732 0.0 54 0.0 0.0 0.0 9.8758152416689 0.0 55 0.0 0.0 0.0 10.159921379433575 0.0 56 0.0 0.0 0.0 10.440453855088002 0.0 57 0.0 0.0 0.0 10.679889216474582 0.0 58 0.0 0.0 0.0 10.987224157955865 0.0 59 0.0 0.0 0.0 11.282051282051283 0.0 60 0.0 0.0 0.0 11.480389529170017 0.0 61 0.0 0.0 0.0 11.743053694273206 0.0 62 0.0 0.0 0.0 11.939605110336817 0.0 63 0.0 0.0 0.0 12.123648709014562 0.0 64 0.0 0.0 0.0 12.354149915125525 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGAAGT 25 2.805802E-6 70.00001 24 TATACGG 25 2.805802E-6 70.00001 30 TACGGAT 25 2.805802E-6 70.00001 32 GTATACG 25 2.805802E-6 70.00001 29 ATACGGA 35 3.6288839E-9 70.00001 31 CGACTTC 25 2.805802E-6 70.00001 48 ACAAATC 20 7.862984E-5 70.0 6 CGACACC 15 0.0022138285 70.0 39 ACTCGTA 15 0.0022138285 70.0 38 AATCTCC 20 7.862984E-5 70.0 9 AGAAGTA 30 1.0065742E-7 70.0 25 CTTTACA 15 0.0022138285 70.0 1 ATTAGGA 15 0.0022138285 70.0 31 GTCAAAC 15 0.0022138285 70.0 33 CACAACT 15 0.0022138285 70.0 7 GAAGTCA 15 0.0022138285 70.0 30 ACTCCGA 15 0.0022138285 70.0 11 TCGGAAC 30 1.0065742E-7 70.0 33 TCAGCAT 15 0.0022138285 70.0 53 CGGAACT 20 7.862984E-5 70.0 34 >>END_MODULE