FastQCFastQC Report
Wed 25 May 2016
SRR1779992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62035
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3682959.368098653985655No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG38716.240025791891674No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT4220.6802611429031998No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3760.6061094543402917No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3760.6061094543402917No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1100.17731925525912792No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG830.13379543805916017No Hit
CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG750.12089949222213267No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTG150.002216380369.9887166
GTATCAA150.002216380369.98871657
GTCTGGC150.002216380369.98871610
CAATAAA150.002216380369.98871661
TGTGGCG301.00950274E-769.98871611
CGCGTCT150.002216380369.9887167
CCGGCGG150.002216380369.98871621
GTGGCGC252.8120903E-669.9887112
TTGTGCG207.8759884E-569.988712
TGTGCGC252.8120903E-669.988713
TGGCGCT453.7289283E-1062.26239813
GTGTGGC352.9332114E-759.99032210
GGCGAGG252.3752681E-455.99096723
AAAAAGG3900.055.6320570
TGCCGGC200.006903541352.53389719
GTAGATA200.00692562852.49153538
TGCGCGT200.00692562852.4915355
TCTGGCG200.00692562852.49153511
TCAATAA200.00692562852.49153560
ATCAGCA200.00692562852.4915359