##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779992_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 62035 Sequences flagged as poor quality 0 Sequence length 76 %GC 81 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.985282501813494 32.0 27.0 32.0 22.0 32.0 2 29.43944547432901 32.0 32.0 32.0 22.0 32.0 3 29.2902393809946 32.0 32.0 32.0 22.0 32.0 4 28.689900862416376 32.0 27.0 32.0 13.0 32.0 5 29.547706939630853 32.0 32.0 32.0 22.0 32.0 6 33.75291367776256 37.0 37.0 37.0 22.0 37.0 7 33.42717820585153 37.0 37.0 37.0 13.0 37.0 8 33.719319738857095 37.0 37.0 37.0 22.0 37.0 9 33.873958249375356 37.0 37.0 37.0 22.0 37.0 10-11 33.610340936568065 37.0 37.0 37.0 22.0 37.0 12-13 33.36406867091158 37.0 37.0 37.0 22.0 37.0 14-15 33.80439268155074 37.0 37.0 37.0 20.0 37.0 16-17 34.1121544289514 37.0 37.0 37.0 24.5 37.0 18-19 33.774401547513506 37.0 37.0 37.0 22.0 37.0 20-21 34.10099943580237 37.0 37.0 37.0 24.5 37.0 22-23 34.2292979769485 37.0 37.0 37.0 24.5 37.0 24-25 33.829628435560565 37.0 37.0 37.0 22.0 37.0 26-27 34.150544047715 37.0 37.0 37.0 24.5 37.0 28-29 34.001894092044814 37.0 37.0 37.0 22.0 37.0 30-31 33.49058595953897 37.0 37.0 37.0 17.5 37.0 32-33 33.860216007092774 37.0 37.0 37.0 22.0 37.0 34-35 33.973369871846536 37.0 37.0 37.0 22.0 37.0 36-37 34.67349883130491 37.0 37.0 37.0 29.5 37.0 38-39 34.813379543805915 37.0 37.0 37.0 29.5 37.0 40-41 34.38942532441364 37.0 37.0 37.0 29.5 37.0 42-43 34.28552430079794 37.0 37.0 37.0 27.0 37.0 44-45 34.14559522850004 37.0 37.0 37.0 27.0 37.0 46-47 34.38577415974853 37.0 37.0 37.0 27.0 37.0 48-49 34.78846618844201 37.0 37.0 37.0 29.5 37.0 50-51 34.997243491577336 37.0 37.0 37.0 32.0 37.0 52-53 34.33257838317079 37.0 37.0 37.0 27.0 37.0 54-55 34.4594180704441 37.0 37.0 37.0 29.5 37.0 56-57 34.34346739743693 37.0 37.0 37.0 27.0 37.0 58-59 34.04812605787056 37.0 37.0 37.0 24.5 37.0 60-61 33.93554445071331 37.0 37.0 37.0 24.5 37.0 62-63 33.588861126783264 37.0 37.0 37.0 22.0 37.0 64-65 33.32471185621021 37.0 37.0 37.0 17.5 37.0 66-67 33.477698073668094 37.0 37.0 37.0 22.0 37.0 68-69 33.761360522285806 37.0 37.0 37.0 22.0 37.0 70-71 33.84356411703071 37.0 37.0 37.0 22.0 37.0 72-73 33.90432014185541 37.0 37.0 37.0 22.0 37.0 74-75 33.41414524058999 37.0 37.0 37.0 17.5 37.0 76 33.85298621745789 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 0.9497457938024709 11101 2 1.6285191629411777 11101 3 -0.23586519625631297 11101 4 2.090221605507395 11101 5 0.1529211472917673 11101 6 1.1258829065106184 11101 7 2.0995735606657036 11101 8 0.6828549009011127 11101 9 0.9843723985653909 11101 10-11 0.6449164628401363 11101 12-13 2.208585719113202 11101 14-15 -0.09160038105385837 11101 16-17 0.18463751288074093 11101 18-19 -0.5498699680703396 11101 20-21 0.16805307549194026 11101 22-23 0.13346056048210642 11101 24-25 0.7305153525416728 11101 26-27 -0.00759090409788854 11101 28-29 0.6591510299917687 11101 30-31 0.8798907365136657 11101 32-33 -0.07524899709210331 11101 34-35 0.3077333540011864 11101 36-37 0.28356152678461655 11101 38-39 -0.9528253786651604 11101 40-41 0.49327775704208676 11101 42-43 -0.23636528402145274 11101 44-45 -0.543841967411467 11101 46-47 -0.004604759256864099 11101 48-49 -0.6933754594576129 11101 50-51 -0.10704895613382348 11101 52-53 0.5969250967612751 11101 54-55 -0.3543765494219997 11101 56-57 0.016702571008195832 11101 58-59 -0.5303595233184168 11101 60-61 -0.09344753735589961 11101 62-63 0.14498022896807328 11101 64-65 1.1913871523122452 11101 66-67 0.19270504202031447 11101 68-69 0.46776556122640756 11101 70-71 0.5520730957030722 11101 72-73 -0.7717050326647978 11101 74-75 0.2252710094660273 11101 76 -1.0190613363256418 11102 1 0.20413175871475175 11102 2 1.380398862189299 11102 3 1.9445859315632354 11102 4 0.8433544375875961 11102 5 1.7281091172165794 11102 6 0.3928001997437036 11102 7 0.6158642874827436 11102 8 -0.19057868305878145 11102 9 0.28763054392879894 11102 10-11 -0.014857973250094858 11102 12-13 0.42725739831119824 11102 14-15 0.3870963608007756 11102 16-17 0.40957485623913925 11102 18-19 -0.48846645929840804 11102 20-21 0.533341296043325 11102 22-23 1.2995006607327326 11102 24-25 1.0168562046720027 11102 26-27 1.0105795219672729 11102 28-29 0.7474968946534233 11102 30-31 2.0872842202229407 11102 32-33 0.45545275729385537 11102 34-35 1.2005904968583252 11102 36-37 0.5266692962081763 11102 38-39 0.4224879045428551 11102 40-41 0.3184657269668918 11102 42-43 0.09195551798355694 11102 44-45 0.4636768295810185 11102 46-47 0.5900067695651927 11102 48-49 -0.6501423767508498 11102 50-51 1.076535003765926 11102 52-53 1.0254965253327057 11102 54-55 -0.5504918376425465 11102 56-57 0.6601862802813798 11102 58-59 0.20898884760890013 11102 60-61 1.175976021541345 11102 62-63 0.6155316074141908 11102 64-65 -0.9921968075875043 11102 66-67 0.46087546808547586 11102 68-69 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2.0572115580983095 11105 8 2.6520461717611923 11105 9 0.6563878029299559 11105 10-11 1.3056995180391127 11105 12-13 0.8735407897165359 11105 14-15 1.379837359639339 11105 16-17 1.3117235205829232 11105 18-19 0.936330288669069 11105 20-21 0.6346769522570312 11105 22-23 0.38602795457709504 11105 24-25 0.6120942999100976 11105 26-27 0.13746686226924965 11105 28-29 -0.23333934228037378 11105 30-31 0.11544914472933243 11105 32-33 0.8660218629849155 11105 34-35 1.3748065247328896 11105 36-37 0.763022374024672 11105 38-39 0.027598241360507814 11105 40-41 0.044946563203829726 11105 42-43 1.101247039470202 11105 44-45 -0.3002604526489492 11105 46-47 0.7113772689844637 11105 48-49 0.8184987382317317 11105 50-51 0.3358265252525712 11105 52-53 0.6335104626149359 11105 54-55 0.9614129242622127 11105 56-57 0.5629156647180835 11105 58-59 0.7876764201989133 11105 60-61 -0.2462074860080179 11105 62-63 -0.636791272957467 11105 64-65 -1.1295371095619515 11105 66-67 0.7645920766801382 11105 68-69 1.3105126729080538 11105 70-71 0.39449928312284044 11105 72-73 -0.10514534075208815 11105 74-75 -0.5443053705083045 11105 76 1.5329283940979792 11106 1 0.4207984253814203 11106 2 0.4732560050464407 11106 3 0.13387164584895217 11106 4 -0.0018836576504988045 11106 5 0.517394831502294 11106 6 -1.1964855145420117 11106 7 0.22062619224465152 11106 8 -1.1131977306778325 11106 9 -0.73141707511882 11106 10-11 -0.47021511610723365 11106 12-13 0.16713835069214866 11106 14-15 -0.9277845915801706 11106 16-17 0.3714796181439013 11106 18-19 1.3823668740349149 11106 20-21 -1.4214206087185772 11106 22-23 -0.4106183868863127 11106 24-25 0.48709429991009756 11106 26-27 -0.5418014304136705 11106 28-29 -0.38821739106085573 11106 30-31 -0.1299776845389644 11106 32-33 0.33461942396053246 11106 34-35 0.13273335400118924 11106 36-37 -0.3388068942680107 11106 38-39 0.05046409501904492 11106 40-41 -0.4422485587473943 11106 42-43 0.6985031370311745 11106 44-45 0.2851054010095879 11106 46-47 -0.011183706625288892 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21612 76 -1.3673585499479373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 5.0 12 17.0 13 47.0 14 75.0 15 124.0 16 258.0 17 373.0 18 454.0 19 464.0 20 435.0 21 495.0 22 569.0 23 626.0 24 794.0 25 812.0 26 912.0 27 958.0 28 1071.0 29 1252.0 30 1457.0 31 1752.0 32 2319.0 33 3312.0 34 5502.0 35 9452.0 36 28496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 75.43805916015152 5.229306036914645 3.6028048682195535 15.729829934714273 2 70.11848150237769 6.020794712662206 16.996856613202223 6.863867171757879 3 69.98629805754815 6.604336261787701 16.963004755380027 6.4463609252841145 4 69.08369873613618 12.34362909466082 10.206022697962341 8.36664947124065 5 68.77246715563795 9.19158539534134 17.630369952446202 4.405577496574514 6 72.19499613102914 17.20241165849884 5.714792365230849 4.887799845241165 7 71.43064399129524 9.27379705005239 6.162650116869509 13.132908841782864 8 71.55799145643589 16.73571371000242 5.588780527121786 6.1175143064399125 9 70.37478842588861 6.401225114854518 4.663496413315063 18.56049004594181 10-11 71.90779398726525 10.91561215442895 10.50052389779963 6.676069960506166 12-13 71.16305311517691 6.522930603691464 11.21141291206577 11.10260336906585 14-15 76.25533972757314 9.700975255903925 6.216651890062062 7.8270331264608695 16-17 76.08849842830661 6.957362779076328 5.8047876198919965 11.149351172725074 18-19 71.57791229074144 6.452860908043105 9.989602559865881 11.979624241349573 20-21 75.78647021367487 6.608526038350246 6.845494772985564 10.759508974989322 22-23 75.75239783992907 6.278713629402756 5.7660997823809135 12.202788748287258 24-25 72.47257574414238 11.318701690188524 5.573511513754443 10.63521105191466 26-27 75.91823904440271 11.066422716391685 6.070815433347573 6.94452280585803 28-29 72.23422261626501 12.122189086805836 9.90811638591118 5.7354719110179735 30-31 75.61256730180223 11.60089628268369 7.16542541187091 5.621111003643164 32-33 77.10046103749558 10.996389076957797 6.060386239803979 5.842763645742657 34-35 76.69783186910615 11.06633352139921 6.643830095913597 5.592004513581043 36-37 77.05247037962441 6.826791327476426 10.478761989199645 5.641976303699525 38-39 76.91625695172081 6.270653663254615 11.518497622309987 5.294591762714597 40-41 75.99339082775852 10.122511485451762 7.9688885306681705 5.915209156121545 42-43 72.34039123391017 10.35069195367094 10.863309932376339 6.445606880042557 44-45 71.87797211251713 6.068348512936246 11.759490610139437 10.294188764407188 46-47 75.68550012089949 5.4034013057145165 8.258241315386474 10.652857257999516 48-49 77.19996776013541 5.288143789796083 11.5418715241396 5.970016925928911 50-51 80.28532280164423 5.578302571129202 8.041428226001452 6.094946401225115 52-53 72.27107496634937 6.111921592017345 11.16072508039881 10.456278361234475 54-55 74.99476102200371 6.165874103328766 7.554606270653663 11.284758604013863 56-57 75.21479809784799 6.587410332876602 7.006528572579995 11.191262996695414 58-59 71.7082567624206 10.424122255537286 10.873875616597351 6.993745365444756 60-61 70.83259450310308 7.078262271298461 11.711130813250584 10.378012412347868 62-63 70.97444990731039 10.975255903925204 10.962359958088177 7.087934230676231 64-65 70.95060773124415 11.359899410000967 11.521101331527872 6.168391527227005 66-67 70.83558342535203 16.44085146168664 6.215089990247362 6.508475122713973 68-69 70.91077617474006 17.077456274683648 5.7257999516402025 6.285967598936085 70-71 71.42177802853227 17.092770210365117 5.55895865237366 5.926493108728943 72-73 72.17054888369468 16.189248005158376 5.412267268477473 6.227935842669461 74-75 75.44169326498373 13.421671986330077 5.5316439371957316 5.604990811490473 76 81.64584508745062 7.357137100024181 5.577496574514387 5.4195212380108 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.5 14 2.5 15 3.0 16 5.5 17 8.0 18 7.5 19 7.5 20 12.5 21 17.0 22 19.5 23 35.5 24 43.0 25 37.0 26 60.0 27 89.5 28 98.0 29 100.0 30 121.5 31 148.5 32 197.0 33 240.0 34 267.0 35 296.5 36 375.5 37 452.0 38 510.5 39 568.5 40 578.0 41 833.5 42 1079.0 43 1968.5 44 3276.5 45 2491.0 46 1287.0 47 1169.5 48 973.5 49 785.0 50 675.0 51 636.0 52 581.5 53 618.0 54 670.0 55 622.0 56 517.5 57 436.5 58 412.0 59 387.5 60 333.5 61 302.5 62 301.0 63 278.5 64 237.5 65 189.0 66 149.5 67 140.0 68 117.0 69 94.5 70 89.5 71 84.0 72 88.5 73 69.5 74 52.0 75 58.0 76 53.0 77 47.0 78 57.0 79 68.0 80 55.0 81 54.5 82 56.0 83 45.0 84 52.5 85 66.5 86 77.5 87 82.0 88 94.5 89 113.0 90 117.0 91 139.0 92 163.0 93 193.0 94 260.0 95 331.0 96 365.0 97 472.0 98 885.5 99 19380.0 100 37568.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.004835979688885306 5 0.0 6 0.004835979688885306 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.059966148142178E-4 20-21 0.002417989844442653 22-23 0.0 24-25 8.059966148142178E-4 26-27 8.059966148142178E-4 28-29 0.0 30-31 0.0016119932296284356 32-33 0.0016119932296284356 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.059966148142178E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.059966148142178E-4 54-55 0.0 56-57 0.0 58-59 0.0016119932296284356 60-61 0.0 62-63 0.0 64-65 0.0016119932296284356 66-67 8.059966148142178E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0016119932296284356 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 62035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.14894817441767 #Duplication Level Percentage of deduplicated Percentage of total 1 74.12657217009382 11.970661723220763 2 5.779596725893392 1.8666881599097285 3 4.96107007386704 2.4034819053759975 4 4.182471551207826 2.7017006528572582 5 3.5635855460171695 2.8774079148867577 6 2.5454182471551206 2.4663496413315062 7 1.597125174685566 1.8054324171838478 8 1.1379516869634656 1.4701378254211332 9 0.5789578758235177 0.8414604658660434 >10 1.4374126572170094 3.5705650036269847 >50 0.029946097025354362 0.34819053759974206 >100 0.039928129367139145 2.0697993068429112 >500 0.0 0.0 >1k 0.009982032341784786 6.240025791891674 >5k 0.0 0.0 >10k+ 0.009982032341784786 59.368098653985655 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 36829 59.368098653985655 No Hit CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG 3871 6.240025791891674 No Hit TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT 422 0.6802611429031998 No Hit CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG 376 0.6061094543402917 No Hit ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC 376 0.6061094543402917 No Hit TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG 110 0.17731925525912792 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 83 0.13379543805916017 No Hit CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG 75 0.12089949222213267 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.01128395260739905 0.0 5 0.0 0.0 0.0 0.03868783751108246 0.0 6 0.0 0.0 0.0 0.03868783751108246 0.0 7 0.0 0.0 0.0 0.03868783751108246 0.0 8 0.0 0.0 0.0 0.03868783751108246 0.0 9 0.0 0.0 0.0 0.05480776980736681 0.0 10 0.0 0.0 0.0 0.05480776980736681 0.0 11 0.0 0.0 0.0 0.06125574272588055 0.0 12 0.0 0.0 0.0 0.06125574272588055 0.0 13 0.0 0.0 0.0 0.06931570887402273 0.0 14 0.0 0.0 0.0 0.08221165471105021 0.0 15 0.0 0.0 0.0 0.08221165471105021 0.0 16 0.0 0.0 0.0 0.08543564117030708 0.0 17 0.0 0.0 0.0 0.09833158700733458 0.0 18 0.0 0.0 0.0 0.10800354638510518 0.0 19 0.0 0.0 0.0 0.12412347868138954 0.0 20 0.0 0.0 0.0 0.1273474651406464 0.0 21 0.0 0.0 0.0 0.14185540420730233 0.0 22 0.0 0.0 0.0 0.20149915370355445 0.0 23 0.0 0.0 0.0 0.20149915370355445 0.0 24 0.0 0.0 0.0 0.22567905214798098 0.0 25 0.0 0.0 0.0 0.23857499798500847 0.0 26 0.0 0.0 0.0 0.23857499798500847 0.0 27 0.0 0.0 0.0 0.2450229709035222 0.0 28 0.0 0.0 0.0 0.2579189167405497 0.0 29 0.0 0.0 0.0 0.28371080841460466 0.0 30 0.0 0.0 0.0 0.3304586120738293 0.0 31 0.0 0.0 0.0 0.35463851051825585 0.0 32 0.0 0.0 0.0 0.37398242927379705 0.0 33 0.0 0.0 0.0 0.39655033448859517 0.0 34 0.0 0.0 0.0 0.45135810429596196 0.0 35 0.0 0.0 0.0 0.5561376642218103 0.0 36 0.0 0.0 0.0 0.6689771902958007 0.0 37 0.0 0.0 0.0 0.9027162085919239 0.0 38 0.0 0.0 0.0 1.1348432336584187 0.0 39 0.0 0.0 0.0 1.4378979608285645 0.0 40 0.0 0.0 0.0 1.736116708309825 0.0 41 0.0 0.0 0.0 2.079471266220682 0.0 42 0.0 0.0 0.0 2.479245587168534 0.0 43 0.0 0.0 0.0 2.7629563955831387 0.0 44 0.0 0.0 0.0 3.1804626420569035 0.0 45 0.0 0.0 0.0 3.4690094301603933 0.0 46 0.0 0.0 0.0 3.938099459982268 0.0 47 0.0 0.0 0.0 4.25566212621907 0.0 48 0.0 0.0 0.0 4.59579269767067 0.0 49 0.0 0.0 0.0 4.874667526396389 0.0 50 0.0 0.0 0.0 5.148706375433223 0.0 51 0.0 0.0 0.0 5.475941001047795 0.0 52 0.0 0.0 0.0 5.700008059966148 0.0 53 0.0 0.0 0.0 5.877327315225276 0.0 54 0.0 0.0 0.0 6.211009913758362 0.0 55 0.0 0.0 0.0 6.473764810187797 0.0 56 0.0 0.0 0.0 6.601112275328443 0.0 57 0.0 0.0 0.0 6.833239300394938 0.0 58 0.0 0.0 0.0 7.065366325461433 0.0 59 0.0 0.0 0.0 7.25396953332796 0.0 60 0.0 0.0 0.0 7.561860240186991 0.0 61 0.0 0.0 0.0 7.700491657935037 0.0 62 0.0 0.0 0.0 7.885870879342307 0.0 63 0.0 0.0 0.0 8.030950270008866 0.0 64 0.0 0.0 0.0 8.20182155234948 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGGTG 15 0.0022163803 69.988716 6 GTATCAA 15 0.0022163803 69.988716 57 GTCTGGC 15 0.0022163803 69.988716 10 CAATAAA 15 0.0022163803 69.988716 61 TGTGGCG 30 1.00950274E-7 69.988716 11 CGCGTCT 15 0.0022163803 69.988716 7 CCGGCGG 15 0.0022163803 69.988716 21 GTGGCGC 25 2.8120903E-6 69.98871 12 TTGTGCG 20 7.8759884E-5 69.98871 2 TGTGCGC 25 2.8120903E-6 69.98871 3 TGGCGCT 45 3.7289283E-10 62.262398 13 GTGTGGC 35 2.9332114E-7 59.990322 10 GGCGAGG 25 2.3752681E-4 55.990967 23 AAAAAGG 390 0.0 55.63205 70 TGCCGGC 20 0.0069035413 52.533897 19 GTAGATA 20 0.006925628 52.491535 38 TGCGCGT 20 0.006925628 52.491535 5 TCTGGCG 20 0.006925628 52.491535 11 TCAATAA 20 0.006925628 52.491535 60 ATCAGCA 20 0.006925628 52.491535 9 >>END_MODULE