SRR1779992_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779992_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62035
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	20428	32.92979769484968	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6595	10.631095349399533	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG	2928	4.719916176352059	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC	2734	4.407189489804143	No Hit
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	1542	2.4856935600870473	No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	759	1.2235028612879826	No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT	723	1.1654711050213589	TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC	471	0.7592488111549931	No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	436	0.702829048117998	No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	327	0.5271217860884984	No Hit
TCATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG	277	0.44652212460707663	No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC	253	0.4078342870959942	No Hit
CAAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	156	0.25147094382203594	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT	131	0.21117111308132505	No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT	122	0.19666317401466912	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG	120	0.19343918755541226	No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC	116	0.18699121463689852	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGCATCTCGTATGCCGT	106	0.17087128234061416	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC	103	0.16603530265172886	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAAAGAATCTCGTATGCCGT	98	0.1579753365035867	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG	95	0.1531393568147014	No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC	86	0.13863141774804547	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC	81	0.13057145159990327	No Hit
CATAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	78	0.12573547191101797	No Hit
CTTATACACATCTCCGAGCCAACGAGACGGGAATGAATCTCGTATGCCGT	74	0.11928749899250424	No Hit
CTTATACAAATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT	72	0.11606351253324737	No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC	72	0.11606351253324737	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGAT	20	7.851697E-5	70.03229	52
ACAAAGG	15	0.0022109076	70.03229	66
ACTGCAT	20	7.851697E-5	70.03229	53
CCAAAGA	15	0.0022109076	70.03229	19
AGAGCCA	15	0.0022109076	70.03229	15
TACTGCA	20	7.851697E-5	70.03229	52
GCTTTAA	15	0.0022109076	70.03229	56
TATCCGA	55	0.0	69.975815	11
ATATCCG	55	0.0	69.975815	10
AATCTGC	15	0.0022180015	69.97581	51
CACATAT	45	5.456968E-12	69.97581	7
AATCACG	25	2.8151717E-6	69.97581	36
ACACATA	50	0.0	69.97581	6
CCGTATT	15	0.0022180015	69.97581	47
TCACGTA	25	2.8151717E-6	69.97581	38
ACATATC	50	0.0	69.97581	8
GACGTAA	15	0.0022180015	69.97581	46
CTTATGA	20	7.883183E-5	69.97581	51
GAATCTA	15	0.0022180015	69.97581	35
AGAGACG	45	5.456968E-12	69.97581	23

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content