FastQCFastQC Report
Wed 25 May 2016
SRR1779991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115537
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3299828.560547703333132No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG27222.3559552351194855No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3040.2631191739442776No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC2860.24753974917126115No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT2550.22070851761773286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACAGC150.00221982770.0000120
AGTCGAC150.00221982770.0000142
CTTTTCG252.382012E-456.00000448
GGGAGAT252.382012E-456.00000441
CGCTTTG252.382012E-456.00000453
TCTGCGG200.00693629552.517
CTATGCC200.00693629552.59
ACTGCCA200.00693629552.533
GCGCGTC200.00693629552.56
CTATCCT200.00693629552.538
CGCGAGT200.00693629552.510
ATCAACG200.00693629552.544
TCAGACT200.00693629552.545
CAAGCGA200.00693629552.521
GCGGCTA200.00693629552.520
CTTTAGT200.00693629552.51
GCGAAGG305.857343E-446.66666862
ACTTATT305.857343E-446.66666822
AAGCGAT305.857343E-446.66666822
AAGCGAA305.857343E-446.66666860
Produced by FastQC (version 0.11.5)