##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779991_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115537 Sequences flagged as poor quality 0 Sequence length 76 %GC 62 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.4995369448748 32.0 27.0 32.0 27.0 32.0 2 30.081566943922727 32.0 32.0 32.0 27.0 32.0 3 29.960739849571997 32.0 32.0 32.0 27.0 32.0 4 29.310264244354624 32.0 32.0 32.0 22.0 32.0 5 29.92799709184071 32.0 32.0 32.0 27.0 32.0 6 34.054943438032836 37.0 37.0 37.0 27.0 37.0 7 34.000666453170844 37.0 37.0 37.0 27.0 37.0 8 33.927849952828964 37.0 37.0 37.0 27.0 37.0 9 34.20205648407004 37.0 37.0 37.0 27.0 37.0 10-11 33.95811731306854 37.0 37.0 37.0 24.5 37.0 12-13 33.83067329080727 37.0 37.0 37.0 22.0 37.0 14-15 34.2864320520699 37.0 37.0 37.0 27.0 37.0 16-17 34.41623029851909 37.0 37.0 37.0 29.5 37.0 18-19 34.01424651843132 37.0 37.0 37.0 24.5 37.0 20-21 34.314280273851665 37.0 37.0 37.0 27.0 37.0 22-23 34.29724676943317 37.0 37.0 37.0 24.5 37.0 24-25 33.90183231345803 37.0 37.0 37.0 22.0 37.0 26-27 34.203835135064956 37.0 37.0 37.0 24.5 37.0 28-29 34.115837350805364 37.0 37.0 37.0 27.0 37.0 30-31 33.857318434787125 37.0 37.0 37.0 22.0 37.0 32-33 34.04449656819894 37.0 37.0 37.0 27.0 37.0 34-35 34.02777897989388 37.0 37.0 37.0 24.5 37.0 36-37 34.5532340289258 37.0 37.0 37.0 29.5 37.0 38-39 34.54663873910522 37.0 37.0 37.0 29.5 37.0 40-41 34.13497840518622 37.0 37.0 37.0 24.5 37.0 42-43 34.03970589508123 37.0 37.0 37.0 27.0 37.0 44-45 33.88218492777206 37.0 37.0 37.0 22.0 37.0 46-47 34.09368860191974 37.0 37.0 37.0 24.5 37.0 48-49 34.42334490249877 37.0 37.0 37.0 27.0 37.0 50-51 34.56180271255096 37.0 37.0 37.0 27.0 37.0 52-53 33.86588279079429 37.0 37.0 37.0 24.5 37.0 54-55 33.9967066827077 37.0 37.0 37.0 24.5 37.0 56-57 33.803881007815676 37.0 37.0 37.0 24.5 37.0 58-59 33.56365493305175 37.0 37.0 37.0 22.0 37.0 60-61 33.38859413001896 37.0 37.0 37.0 17.5 37.0 62-63 33.14559405212183 37.0 37.0 37.0 17.5 37.0 64-65 33.08907536113972 37.0 34.5 37.0 17.5 37.0 66-67 33.180665068333084 37.0 37.0 37.0 17.5 37.0 68-69 33.345716090949225 37.0 37.0 37.0 22.0 37.0 70-71 33.355816751343724 37.0 37.0 37.0 22.0 37.0 72-73 33.40567956585336 37.0 37.0 37.0 22.0 37.0 74-75 33.29253832105732 37.0 37.0 37.0 17.5 37.0 76 33.13468412716273 37.0 37.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.1710099809970345 11101 2 0.9110488713612916 11101 3 -0.022524538275515482 11101 4 0.5968127071625666 11101 5 0.9154058313572229 11101 6 0.4903673361958667 11101 7 1.2323650825757042 11101 8 0.8486466655017182 11101 9 0.8562930535293773 11101 10-11 1.3619533882770725 11101 12-13 0.8484540840544668 11101 14-15 1.2760873647956643 11101 16-17 1.2253002399253177 11101 18-19 1.213925165585053 11101 20-21 0.5351580482271672 11101 22-23 0.5766139411087039 11101 24-25 0.7760766055991155 11101 26-27 1.7229766375548436 11101 28-29 1.6912017070036995 11101 30-31 0.8785286755344046 11101 32-33 0.82772744415945 11101 34-35 0.6421998566348748 11101 36-37 1.1472420147412663 11101 38-39 1.0075521084212156 11101 40-41 1.7170025169118972 11101 42-43 0.9994053370217344 11101 44-45 1.3026946291546437 11101 46-47 0.2802736985856811 11101 48-49 1.3692319053049289 11101 50-51 1.017321686540626 11101 52-53 1.036762522512639 11101 54-55 0.80975397751304 11101 56-57 1.5001216617045685 11101 58-59 1.4271377700901695 11101 60-61 1.2815230673905447 11101 62-63 0.9594642082038476 11101 64-65 1.7270011861907548 11101 66-67 0.9185172265094863 11101 68-69 0.6884377958501702 11101 70-71 0.5860511168375169 11101 72-73 1.337019722429396 11101 74-75 0.5881825113442432 11101 76 0.6908152619837225 11102 1 -0.6616823266952743 11102 2 0.006775367087787032 11102 3 -0.28641342716440477 11102 4 -1.3709223355724767 11102 5 -0.23181639086499928 11102 6 0.5709228917514224 11102 7 -1.1830195328089133 11102 8 -0.8417379498828979 11102 9 -1.2390060917697667 11102 10-11 -0.06507652625285232 11102 12-13 -0.871417710817326 11102 14-15 -1.1750878488795493 11102 16-17 -1.132178392553314 11102 18-19 -1.0868226976628108 11102 20-21 -1.1247778492087264 11102 22-23 -0.6364202469254892 11102 24-25 -0.9276627106401918 11102 26-27 -0.16762165304344734 11102 28-29 -0.41734530154331395 11102 30-31 -1.6853602133544783 11102 32-33 -0.37408879515678706 11102 34-35 -0.9164539895189705 11102 36-37 0.3061095361087851 11102 38-39 -0.8015290881599739 11102 40-41 -0.3605615856521993 11102 42-43 -0.43638526126886035 11102 44-45 -0.8563865674265472 11102 46-47 -1.1430168997049108 11102 48-49 -0.3018151032420775 11102 50-51 -0.33641762969869404 11102 52-53 -0.027233203983094256 11102 54-55 -0.6313785011194426 11102 56-57 0.09221567879858128 11102 58-59 0.24455230000469896 11102 60-61 -0.06484445397698124 11102 62-63 -1.4158563046166606 11102 64-65 -0.1806911215015532 11102 66-67 0.7520642350564941 11102 68-69 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11103 48-49 -1.2510110096748264 11103 50-51 -0.6474556413946004 11103 52-53 -1.0115168297140897 11103 54-55 -0.3620071560902005 11103 56-57 0.1813969653482843 11103 58-59 -1.1388541327438375 11103 60-61 -1.2125052726904286 11103 62-63 -1.3577422695289414 11103 64-65 -1.5030595425541833 11103 66-67 -1.4204503848265482 11103 68-69 -0.5956715158907215 11103 70-71 -1.2945156847819206 11103 72-73 -0.6582231925503592 11103 74-75 -0.0860077720565613 11103 76 -0.5282130780972523 11104 1 0.1833176733047246 11104 2 0.34899758931000946 11104 3 0.6041421283911497 11104 4 -0.38370011335025467 11104 5 -0.07126083530944527 11104 6 -0.8562993304708044 11104 7 0.2703138005244199 11104 8 -0.9800712832162333 11104 9 -0.4534505362142127 11104 10-11 -0.41702097069729405 11104 12-13 -0.09975104415066482 11104 14-15 -1.3664767377684441 11104 16-17 -0.21828950366442257 11104 18-19 0.32623285789274803 11104 20-21 0.004388817457936511 11104 22-23 0.19302419751895883 11104 24-25 -0.23571826619575376 11104 26-27 0.2501561247343247 11104 28-29 -0.5595675237655371 11104 30-31 -0.44147132446558857 11104 32-33 0.3928556492876538 11104 34-35 -0.07062065618563906 11104 36-37 -0.5025015750023201 11104 38-39 -0.13347353260441963 11104 40-41 -0.5455615856522016 11104 42-43 -0.521107483491086 11104 44-45 -0.5783310118709934 11104 46-47 -0.30023912192713453 11104 48-49 0.29179600786903137 11104 50-51 0.5588601480790842 11104 52-53 -0.9652887595386446 11104 54-55 0.09334372110278366 11104 56-57 -0.07833987675697784 11104 58-59 0.5681634111158118 11104 60-61 0.43126665713412393 11104 62-63 -0.25335630461665914 11104 64-65 -0.8873577881682166 11104 66-67 0.5092864572787121 11104 68-69 -0.9320750246626446 11104 70-71 -0.11241042162401982 11104 72-73 -0.29374950833983604 11104 74-75 0.27074661390834365 11104 76 1.8538921850606442 11105 1 -0.5366823266952743 11105 2 0.7161770764894975 11105 3 0.18260366685268892 11105 4 0.23271014306000382 11105 5 0.25643147238286446 11105 6 0.7108801567086829 11105 7 0.9323650825756999 11105 8 0.5204415372965912 11105 9 1.4768058740421992 11105 10-11 0.4375944139180916 11105 12-13 1.529223314823696 11105 14-15 0.05301044171874025 11105 16-17 0.670172034797119 11105 18-19 1.4703354219953155 11105 20-21 0.5466965097656313 11105 22-23 0.8240498385446031 11105 24-25 0.5760766055991127 11105 26-27 0.033233047811251026 11105 28-29 0.5206888864908734 11105 30-31 1.322118419124152 11105 32-33 0.5623428287748382 11105 34-35 -0.3411334766984595 11105 36-37 0.08442150192075104 11105 38-39 0.3293469802160942 11105 40-41 0.8477717476811293 11105 42-43 0.4135079011242979 11105 44-45 0.01166898812900996 11105 46-47 0.054632672944656235 11105 48-49 0.8846165206895407 11105 50-51 -0.24934498012603967 11105 52-53 -0.190160554410447 11105 54-55 0.48667705443611453 11105 56-57 0.14242935401225765 11105 58-59 0.29636853932094454 11105 60-61 0.2545999904674616 11105 62-63 0.5889513876910257 11105 64-65 0.159052468242038 11105 66-67 0.515953123945387 11105 68-69 1.256386513798894 11105 70-71 1.0027177835041812 11105 72-73 0.3203530557627303 11105 74-75 0.6702337933955249 11105 76 0.1856870568555138 11106 1 -0.2639550539680009 11106 2 -0.42901106869864947 11106 3 -0.282264798015774 11106 4 -0.25313734278748257 11106 5 -0.8845941686427778 11106 6 0.6695881154166443 11106 7 0.1806168308274536 11106 8 -1.2134911966361415 11106 9 -1.474576077339755 11106 10-11 0.33977556609924875 11106 12-13 -0.640357104756724 11106 14-15 -0.5074291187208217 11106 16-17 -0.2633111486860713 11106 18-19 -1.1132476615877778 11106 20-21 -0.7137930007238822 11106 22-23 -1.3223870578922927 11106 24-25 0.06358909311160232 11106 26-27 -0.19070967613146905 11106 28-29 0.2565363723383598 11106 30-31 -0.38251028550455146 11106 32-33 0.10904612547813741 11106 34-35 -0.4481098336748133 11106 36-37 -0.4654452979460473 11106 38-39 0.04921045007956337 11106 40-41 -0.5288516289422418 11106 42-43 0.016987754604151917 11106 44-45 -0.35304962658960903 11106 46-47 -1.2553906370786478 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0.5065525211307218 11107 28-29 0.19827031407230322 11107 30-31 1.5312313782371092 11107 32-33 1.3513241177561284 11107 34-35 1.2811811456161593 11107 36-37 0.15389482139406852 11107 38-39 0.453913854782968 11107 40-41 1.0234474233568065 11107 42-43 -0.3044408168244175 11107 44-45 0.2909482674082895 11107 46-47 1.2777788960908865 11107 48-49 0.24116537723840992 11107 50-51 0.054535823754761736 11107 52-53 -0.03943290368278696 11107 54-55 0.7501905679496303 11107 56-57 0.32382228540518554 11107 58-59 0.26050575345815474 11107 60-61 0.29514053100800197 11107 62-63 0.79304009177973 11107 64-65 1.1503899595795275 11107 66-67 0.9979351059273611 11107 68-69 -0.014057006644627279 11107 70-71 0.3989409297273312 11107 72-73 0.2427369781466453 11107 74-75 -0.6939380707763405 11107 76 -0.3482699771015163 11108 1 -0.35096804098098744 11108 2 0.8385213988338194 11108 3 -0.23681025256123078 11108 4 0.7001094104592696 11108 5 -0.21316559721420703 11108 6 -0.23820409237556106 11108 7 0.3598376100482312 11108 8 -0.6524522355971811 11108 9 -1.2953552981189773 11108 10-11 -0.1303543040306181 11108 12-13 -0.49879866319828636 11108 14-15 -0.07885769014939115 11108 16-17 0.7983771630022432 11108 18-19 0.8348042864641769 11108 20-21 -0.04561118254206775 11108 22-23 -0.34316627867151794 11108 24-25 0.35904363856614907 11108 26-27 0.36967993425813717 11108 28-29 0.24614676194875074 11108 30-31 0.5713858183915548 11108 32-33 0.6233318397638499 11108 34-35 -0.26109684666182886 11108 36-37 -0.4115491940499467 11108 38-39 0.02843122930034525 11108 40-41 -0.4093711094617234 11108 42-43 0.15270204031843804 11108 44-45 0.14500232146233571 11108 46-47 -0.9450010266890416 11108 48-49 -0.06868018260716013 11108 50-51 0.057431576650515126 11108 52-53 -0.2929078071576967 11108 54-55 0.05810562586468393 11108 56-57 0.9688029803858811 11108 58-59 0.1634015063538996 11108 60-61 0.8545999904674559 11108 62-63 0.22759607633571477 11108 64-65 -0.2878339786444144 11108 66-67 0.9278578858501376 11108 68-69 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21611 48-49 -0.37511757237788146 21611 50-51 -0.30015219759992817 21611 52-53 -1.1452887595386514 21611 54-55 -0.43307603198363864 21611 56-57 -0.01735222243598855 21611 58-59 -0.5046760950570288 21611 60-61 -1.1126839601498233 21611 62-63 -0.0632328478265407 21611 64-65 -0.5634071708842683 21611 66-67 -0.9408369995114114 21611 68-69 -0.8654083579959817 21611 70-71 -0.7240153598956311 21611 72-73 0.0556332077095405 21611 74-75 -0.3549323984373274 21611 76 0.20895391345570857 21612 1 0.20722011232911441 21612 2 -0.9701894025599103 21612 3 -0.4981342943730773 21612 4 0.44784460209690025 21612 5 -0.46996002230131495 21612 6 -0.8169497369748697 21612 7 -0.9269219718333019 21612 8 -0.6496647791511876 21612 9 -0.059466796376810294 21612 10-11 0.09769447645717833 21612 12-13 -0.5960112067522871 21612 14-15 -0.7077775507765693 21612 16-17 -1.1284521052904353 21612 18-19 -0.28279153235115473 21612 20-21 -1.1090258166884013 21612 22-23 -0.7478701114241275 21612 24-25 -0.38311664017949454 21612 26-27 0.19145693774245842 21612 28-29 -0.6212748408387085 21612 30-31 -0.9031786415387657 21612 32-33 -0.7045427246960827 21612 34-35 0.16433869340785634 21612 36-37 0.6375797258106886 21612 38-39 0.3083963860947634 21612 40-41 -0.018081910855457295 21612 42-43 -0.07273349975124432 21612 44-45 -0.8806074346352233 21612 46-47 -1.219913918675104 21612 48-49 -1.193244642537472 21612 50-51 -0.2126032665550639 21612 52-53 -0.16154892214027683 21612 54-55 -1.818200994344373 21612 56-57 -1.3505349987081985 21612 58-59 -1.3176089466077698 21612 60-61 -1.1173512290447363 21612 62-63 -1.6074213452670705 21612 64-65 -0.7782520971113058 21612 66-67 -0.7421769573554329 21612 68-69 -0.5788229921423209 21612 70-71 -1.3060689582093943 21612 72-73 -1.0608226790715491 21612 74-75 -1.4157574511323041 21612 76 0.9480385265240585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 8.0 12 34.0 13 46.0 14 85.0 15 137.0 16 225.0 17 289.0 18 370.0 19 422.0 20 546.0 21 706.0 22 917.0 23 1171.0 24 1473.0 25 1822.0 26 2026.0 27 2273.0 28 2541.0 29 2829.0 30 3415.0 31 4126.0 32 5122.0 33 7612.0 34 12557.0 35 25405.0 36 39378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 58.96292962427621 13.841453387226602 6.83417433376321 20.36144265473398 2 44.48445086855293 15.207249625661042 24.845720418567215 15.462579087218813 3 43.18270337640755 16.174039485186565 23.56561101638436 17.077646122021516 4 40.20739022426881 15.743826332326389 24.31208939591971 19.73669404748509 5 40.380137964461596 23.542241879224836 26.19939932662264 9.878220829690921 6 49.74292836616695 27.47637018315272 13.27164768202749 9.50905376865284 7 48.68483689207787 20.99587145243515 14.64379376303695 15.675497892450037 8 47.00745215818309 27.360066472212363 13.600837826843348 12.031643542761193 9 46.90358932636298 12.917939707626127 13.60256887404035 26.575902091970537 10-11 47.51205241610912 19.019448315258316 19.158364852817712 14.310134415814849 12-13 47.10049594502194 16.67517764871859 18.776236184079558 17.448090222179907 14-15 48.17114863636757 17.921964392359158 15.857257848135228 18.049629123138043 16-17 47.98765763348538 18.944580523987987 16.106095882704242 16.961665959822394 18-19 45.95277662373633 18.886753912200525 18.033340257582054 17.127129206481097 20-21 47.80890804597701 18.276987259382356 17.123667082121592 16.790437612519042 22-23 48.02747171901642 18.09506911205934 16.59511671585726 17.28234245306698 24-25 46.167195364195415 20.05400937374227 17.294251550389276 16.484543711673037 26-27 47.46659049993075 19.716365461847392 17.3820281124498 15.435015925772053 28-29 46.438772330702115 20.014973687854866 18.714513225315052 14.831740756127958 30-31 47.21380719095677 20.28537054027386 17.810341544480412 14.690480724288953 32-33 47.52346701634577 20.41468132895369 17.16110772107033 14.900743933630215 34-35 47.94178488276483 19.48856210564581 17.833680985312064 14.735972026277297 36-37 48.13284113678362 17.887853622015555 19.194799911716213 14.78450532948462 38-39 47.674297831825854 18.059462500540963 19.54169732115809 14.724542346475095 40-41 47.57002146517103 18.527558509901677 18.816213128375573 15.086206896551724 42-43 45.89951097070152 18.641969965811224 20.05626000778985 15.402259055697407 44-45 45.29458095674978 17.292295974449743 20.527623185646156 16.885499883154313 46-47 46.582219317871996 17.036754936794317 19.350329552388658 17.030696192945026 48-49 46.63224767823294 17.10793944796905 21.029626872776685 15.230186001021318 50-51 47.27510353870855 17.431720711636583 19.229421005483115 16.063754744171753 52-53 44.401744910677195 17.790991552416564 20.984368508516827 16.82289502838942 54-55 45.41504559204746 17.794963452791563 18.87385262538354 17.91613832977743 56-57 45.65656740510575 17.82467012589095 18.604077499318397 17.914684969684906 58-59 44.17300310294239 19.221797629300085 20.30111956930987 16.304079698447662 60-61 44.339667550947105 18.55907007742142 19.978967685536606 17.12229468609487 62-63 44.273596745574935 19.111091876920412 19.684511187086166 16.930800190418488 64-65 45.15341671355 19.077768641537197 19.368589604881635 16.40022504003116 66-67 44.93698824588433 20.97045025706718 17.376702961898662 16.715858535149827 68-69 45.027048080668195 21.236421863504564 16.93599342190678 16.80053663392046 70-71 45.87657419829489 21.435062967931795 16.799671095339075 15.888691738434241 72-73 45.773575107110396 21.76872809105466 16.319297182671917 16.138399619163025 74-75 47.71559764051119 20.558539298125652 16.126058675622225 15.599804385740931 76 50.06967585580128 18.204872982213182 16.214134245033975 15.511316916951573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 2.0 8 3.0 9 3.5 10 7.5 11 9.5 12 8.0 13 10.5 14 19.0 15 22.0 16 28.5 17 38.0 18 67.5 19 97.0 20 102.5 21 108.0 22 143.0 23 203.0 24 285.5 25 343.0 26 407.5 27 539.0 28 671.0 29 736.0 30 826.0 31 1057.0 32 1363.5 33 1529.0 34 1715.5 35 2094.5 36 2392.0 37 2497.0 38 2779.0 39 3262.0 40 3686.0 41 4266.0 42 4623.0 43 5208.5 44 6347.0 45 5802.5 46 4705.0 47 4567.0 48 4248.0 49 3909.0 50 3751.0 51 3389.5 52 2791.0 53 2544.0 54 2534.0 55 2362.5 56 2087.0 57 1771.0 58 1559.0 59 1397.0 60 1160.5 61 981.5 62 877.0 63 767.0 64 624.5 65 551.5 66 449.0 67 387.0 68 381.5 69 367.5 70 345.0 71 331.0 72 302.5 73 231.5 74 185.0 75 181.0 76 174.5 77 170.5 78 147.5 79 122.0 80 119.5 81 100.5 82 99.5 83 115.0 84 99.0 85 72.0 86 69.0 87 77.0 88 86.0 89 98.0 90 98.5 91 106.0 92 116.0 93 125.5 94 150.0 95 218.0 96 271.0 97 331.5 98 589.0 99 17121.5 100 33457.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.005193141591005479 5 0.0 6 0.004327617992504566 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.655235985009131E-4 20-21 8.655235985009131E-4 22-23 0.0 24-25 0.0012982853977513698 26-27 8.655235985009131E-4 28-29 8.655235985009131E-4 30-31 0.0025965707955027395 32-33 0.002163808996252283 34-35 0.0 36-37 4.3276179925045655E-4 38-39 0.0017310471970018262 40-41 8.655235985009131E-4 42-43 0.0017310471970018262 44-45 0.0 46-47 0.0012982853977513698 48-49 0.0 50-51 4.3276179925045655E-4 52-53 8.655235985009131E-4 54-55 0.0012982853977513698 56-57 4.3276179925045655E-4 58-59 0.0012982853977513698 60-61 4.3276179925045655E-4 62-63 0.0017310471970018262 64-65 0.0017310471970018262 66-67 0.0025965707955027395 68-69 0.0017310471970018262 70-71 0.0017310471970018262 72-73 0.0017310471970018262 74-75 0.003029332594753196 76 0.0017310471970018262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 115537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.00251001843565 #Duplication Level Percentage of deduplicated Percentage of total 1 67.26575121163167 20.18141374624579 2 6.961112393261019 4.177016886365407 3 5.680244634202631 5.112647896344894 4 5.235979690745442 6.283701325116629 5 4.079159935379645 6.119251841401456 6 3.4473805677359795 6.205804201251547 7 2.4953842603277177 5.240745388923029 8 1.6097392107085158 3.863697343708076 9 1.1337410570043849 3.06135696789773 >10 2.0684283406415878 7.919540926283355 >50 0.005769674590353105 0.09174550144109679 >100 0.01153934918070621 0.826575036568372 >500 0.0 0.0 >1k 0.0028848372951765524 2.3559552351194855 >5k 0.0 0.0 >10k+ 0.0028848372951765524 28.560547703333132 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 32998 28.560547703333132 No Hit CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG 2722 2.3559552351194855 No Hit CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG 304 0.2631191739442776 No Hit ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC 286 0.24753974917126115 No Hit TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT 255 0.22070851761773286 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.655235985009131E-4 0.0 2 0.0 0.0 0.0 8.655235985009131E-4 0.0 3 0.0 0.0 0.0 0.0017310471970018262 0.0 4 0.0 0.0 0.0 0.0077897123865082185 0.0 5 0.0 0.0 0.0 0.01904151916702009 0.0 6 0.0 0.0 0.0 0.01904151916702009 0.0 7 0.0 0.0 0.0 0.01904151916702009 0.0 8 0.0 0.0 0.0 0.01904151916702009 0.0 9 0.0 0.0 0.0 0.01904151916702009 0.0 10 0.0 0.0 0.0 0.02510018435652648 0.0 11 0.0 0.0 0.0 0.02510018435652648 0.0 12 0.0025965707955027395 0.0 0.0 0.02510018435652648 0.0 13 0.0025965707955027395 0.0 0.0 0.02510018435652648 0.0 14 0.0025965707955027395 0.0 0.0 0.02510018435652648 0.0 15 0.0025965707955027395 0.0 0.0 0.0328898967430347 0.0 16 0.0025965707955027395 0.0 0.0 0.0328898967430347 0.0 17 0.0025965707955027395 0.0 0.0 0.033755420341535614 0.0 18 0.0025965707955027395 0.0 0.0 0.033755420341535614 0.0 19 0.0025965707955027395 0.0 0.0 0.039814085531042 0.0 20 0.0025965707955027395 0.0 0.0 0.048469321516051135 0.0 21 0.0025965707955027395 0.0 0.0 0.05539351030405844 0.0 22 0.0025965707955027395 0.0 0.0 0.11251806780511871 0.0 23 0.0025965707955027395 0.0 0.0 0.11771120939612419 0.0 24 0.0025965707955027395 0.0 0.0 0.15406320053316253 0.0 25 0.0025965707955027395 0.0 0.0 0.15406320053316253 0.0 26 0.0025965707955027395 0.0 0.0 0.15579424773016437 0.0 27 0.0025965707955027395 0.0 0.0 0.1852220500791954 0.0 28 0.0025965707955027395 0.0 0.0 0.2241706120117365 0.0 29 0.0025965707955027395 0.0 0.0 0.25532946155776937 0.0 30 0.0025965707955027395 0.0 0.0 0.31937820784683696 0.0 31 0.0025965707955027395 0.0 0.0 0.35832676977937805 0.0 32 0.0025965707955027395 0.0 0.0 0.43103075205345476 0.0 33 0.0025965707955027395 0.0 0.0 0.5660524334195972 0.0 34 0.0025965707955027395 0.0 0.0 0.6699152652397068 0.0 35 0.0025965707955027395 0.0 0.0 0.8248439893713702 0.0 36 0.0025965707955027395 0.0 0.0 1.0697871677471287 0.0 37 0.0025965707955027395 0.0 0.0 1.4722556410500531 0.0 38 0.0025965707955027395 0.0 0.0 1.9188658178765243 0.0 39 0.0025965707955027395 0.0 0.0 2.49270796368263 0.0 40 0.0025965707955027395 0.0 0.0 3.1920510312713675 0.0 41 0.0025965707955027395 0.0 0.0 3.845521348139557 0.0 42 0.0025965707955027395 0.0 0.0 4.614106303608368 0.0 43 0.0025965707955027395 0.0 0.0 5.346339267940141 0.0 44 0.0025965707955027395 0.0 0.0 5.955667881284783 0.0 45 0.0025965707955027395 0.0 0.0 6.747621973913119 0.0 46 0.0025965707955027395 0.0 0.0 7.481585985441893 0.0 47 0.0025965707955027395 0.0 0.0 8.108225070756554 0.0 48 0.0025965707955027395 0.0 0.0 8.750443580844232 0.0 49 0.0025965707955027395 0.0 0.0 9.442862459644962 0.0 50 0.0025965707955027395 0.0 0.0 9.99073889749604 0.0 51 0.0025965707955027395 0.0 0.0 10.688350917887776 0.0 52 0.0025965707955027395 0.0 0.0 11.334031522369457 0.0 53 0.0025965707955027395 0.0 0.0 11.897487384993552 0.0 54 0.0025965707955027395 0.0 0.0 12.37006326977505 0.0 55 0.0025965707955027395 0.0 0.0 12.977660835922691 0.0 56 0.0025965707955027395 0.0 0.0 13.512554419796256 0.0 57 0.0025965707955027395 0.0 0.0 13.950509360637717 0.0 58 0.0025965707955027395 0.0 0.0 14.423950769017717 0.0 59 0.0025965707955027395 0.0 0.0 14.864502280654682 0.0 60 0.0025965707955027395 0.0 0.0 15.312843504678154 0.0 61 0.0025965707955027395 0.0 0.0 15.689346270026052 0.0 62 0.0025965707955027395 0.0 0.0 16.087487125336473 0.0 63 0.0025965707955027395 0.0 0.0 16.611128902429524 0.0 64 0.0025965707955027395 0.0 0.0 17.12957753793157 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACAGC 15 0.002219827 70.00001 20 AGTCGAC 15 0.002219827 70.00001 42 CTTTTCG 25 2.382012E-4 56.000004 48 GGGAGAT 25 2.382012E-4 56.000004 41 CGCTTTG 25 2.382012E-4 56.000004 53 TCTGCGG 20 0.006936295 52.5 17 CTATGCC 20 0.006936295 52.5 9 ACTGCCA 20 0.006936295 52.5 33 GCGCGTC 20 0.006936295 52.5 6 CTATCCT 20 0.006936295 52.5 38 CGCGAGT 20 0.006936295 52.5 10 ATCAACG 20 0.006936295 52.5 44 TCAGACT 20 0.006936295 52.5 45 CAAGCGA 20 0.006936295 52.5 21 GCGGCTA 20 0.006936295 52.5 20 CTTTAGT 20 0.006936295 52.5 1 GCGAAGG 30 5.857343E-4 46.666668 62 ACTTATT 30 5.857343E-4 46.666668 22 AAGCGAT 30 5.857343E-4 46.666668 22 AAGCGAA 30 5.857343E-4 46.666668 60 >>END_MODULE