FastQCFastQC Report
Wed 25 May 2016
SRR1779991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115537
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT1792615.515376026727367Illumina PCR Primer Index 6 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45583.945056561967162No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC26972.334317145156963No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG24852.150826142274769No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT12071.044686983390602Illumina PCR Primer Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT6390.5530695794420835Illumina PCR Primer Index 6 (95% over 24bp)
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT5460.4725758847814986Illumina PCR Primer Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC3950.3418818214078607No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3340.289084881899305Illumina PCR Primer Index 6 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT2300.19907042765521Illumina PCR Primer Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC2230.19301176246570362Illumina PCR Primer Index 6 (95% over 22bp)
TCATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG2070.17916338488968903No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT1670.1445424409496525TruSeq Adapter, Index 6 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT1180.10213178462310775Illumina PCR Primer Index 6 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC1170.10126626102460684Illumina PCR Primer Index 6 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTC150.002211498870.06631522
CGTCAGC150.002211498870.06631524
ATCTCGG150.002219116870.0056319
CCGATGC150.002219116870.0056333
CCGGAAT150.002219116870.005633
CGTCTTA252.830755E-669.9753348
GCCGACA207.9123114E-569.9753346
ATGATCT150.00222293369.9753241
CGACGTT150.00222293369.9753214
ACGTTAC150.00222293369.9753216
GCGAACT150.00222293369.9753262
TACATGC150.00222293369.9753236
GATGCAT150.00222293369.9753235
CCGACGT150.00222293369.9753213
GTTGGAG150.00222293369.9753211
AGGGGGG25250.064.4327370
AGAACGG352.9331204E-760.05684325
TGACGTC601.8189894E-1258.31276745
GCCACTA252.3658024E-456.07736631
CACGTAT252.3861646E-455.98026339
Produced by FastQC (version 0.11.5)