##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779991_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115537 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.486303089053724 32.0 27.0 32.0 22.0 32.0 2 29.462319430139264 32.0 32.0 32.0 22.0 32.0 3 28.540181933060406 32.0 27.0 32.0 13.0 32.0 4 30.778971238650822 32.0 32.0 32.0 27.0 32.0 5 28.789028622865402 32.0 27.0 32.0 22.0 32.0 6 34.423197763487025 37.0 37.0 37.0 27.0 37.0 7 34.14586669205536 37.0 37.0 37.0 27.0 37.0 8 33.837307529189786 37.0 32.0 37.0 27.0 37.0 9 33.88576819546985 37.0 37.0 37.0 27.0 37.0 10-11 34.575629452037006 37.0 37.0 37.0 27.0 37.0 12-13 34.45059591299757 37.0 37.0 37.0 27.0 37.0 14-15 34.40375810346469 37.0 37.0 37.0 27.0 37.0 16-17 33.75731583821633 37.0 34.5 37.0 24.5 37.0 18-19 34.10178124756571 37.0 37.0 37.0 27.0 37.0 20-21 34.35193487800444 37.0 37.0 37.0 27.0 37.0 22-23 34.14129239983728 37.0 37.0 37.0 27.0 37.0 24-25 34.10881362680354 37.0 37.0 37.0 27.0 37.0 26-27 34.20386110077291 37.0 37.0 37.0 24.5 37.0 28-29 34.138630914771895 37.0 37.0 37.0 27.0 37.0 30-31 33.98452011044081 37.0 37.0 37.0 27.0 37.0 32-33 34.11111159195755 37.0 37.0 37.0 27.0 37.0 34-35 33.806901685174445 37.0 34.5 37.0 24.5 37.0 36-37 34.2183586210478 37.0 37.0 37.0 27.0 37.0 38-39 34.28140335996261 37.0 37.0 37.0 27.0 37.0 40-41 33.937738559941835 37.0 37.0 37.0 24.5 37.0 42-43 33.92031556990402 37.0 37.0 37.0 24.5 37.0 44-45 33.95496247955201 37.0 37.0 37.0 27.0 37.0 46-47 33.47441512242831 37.0 34.5 37.0 22.0 37.0 48-49 32.940097111747754 37.0 32.0 37.0 17.5 37.0 50-51 33.13606896492033 37.0 32.0 37.0 22.0 37.0 52-53 32.808944320866914 37.0 32.0 37.0 17.5 37.0 54-55 32.613898577944724 37.0 32.0 37.0 13.0 37.0 56-57 32.53959770463142 37.0 32.0 37.0 13.0 37.0 58-59 32.75349022391096 37.0 32.0 37.0 17.5 37.0 60-61 32.83247357989215 37.0 32.0 37.0 17.5 37.0 62-63 33.21191479785696 37.0 32.0 37.0 22.0 37.0 64-65 33.33750659961744 37.0 32.0 37.0 22.0 37.0 66-67 33.17451552316574 37.0 32.0 37.0 22.0 37.0 68-69 32.8191098955313 37.0 32.0 37.0 17.5 37.0 70-71 31.102330855050763 37.0 29.5 37.0 13.0 37.0 72-73 32.059188831283485 37.0 32.0 37.0 13.0 37.0 74-75 32.7503440456304 37.0 32.0 37.0 13.0 37.0 76 32.650008222474185 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.2330328905202528 11101 2 1.0136702032996965 11101 3 -0.5722124574040102 11101 4 0.1775971135263461 11101 5 -0.23075025135968374 11101 6 0.5222397975683748 11101 7 0.21433464948629677 11101 8 0.36748681581400433 11101 9 0.8256057554551504 11101 10-11 0.6510094292114132 11101 12-13 -0.7399740399885459 11101 14-15 -0.04430199414763081 11101 16-17 0.7686385323823828 11101 18-19 0.1006312729810972 11101 20-21 1.2636660896911778 11101 22-23 0.4151040941641355 11101 24-25 0.534717985210186 11101 26-27 0.6662198728239943 11101 28-29 -0.0695349647198924 11101 30-31 0.20514404723000723 11101 32-33 0.14572240031686334 11101 34-35 -0.4783552586770057 11101 36-37 -0.06382956476128498 11101 38-39 0.001576331171420975 11101 40-41 0.6094959912055842 11101 42-43 0.19447367619171985 11101 44-45 0.09793016713209823 11101 46-47 0.7018764850830195 11101 48-49 1.1378042351734123 11101 50-51 0.011528260191894901 11101 52-53 -0.25571444829778045 11101 54-55 0.8241485850866184 11101 56-57 0.11422017035069132 11101 58-59 0.29437904355201283 11101 60-61 0.5491701690951487 11101 62-63 -0.12463228958844752 11101 64-65 0.3545163607688053 11101 66-67 0.1464441241751615 11101 68-69 -0.9800361303169076 11101 70-71 0.2372647737954381 11101 72-73 0.830509787084587 11101 74-75 0.43380959818545506 11101 76 0.710764503829111 11102 1 -0.6289730614604245 11102 2 0.4023454169749101 11102 3 0.26261660242504803 11102 4 0.2098620707913028 11102 5 -1.030109225718661 11102 6 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-0.6115991983022084 11103 44-45 -0.12261639157235749 11103 46-47 0.18831373204658064 11103 48-49 -0.1464062911423838 11103 50-51 1.7886537662647726 11103 52-53 0.08628960028521959 11103 54-55 1.6149380587708322 11103 56-57 1.0658396035490725 11103 58-59 0.11877175610261759 11103 60-61 -0.4449593855607219 11103 62-63 0.4945984796423204 11103 64-65 0.4143341745339981 11103 66-67 -0.07582308230256274 11103 68-69 1.2276561773754011 11103 70-71 -0.7854072909818868 11103 72-73 0.08961909882548014 11103 74-75 0.039781258104483186 11103 76 1.4929507386469254 11104 1 0.3515824940951333 11104 2 -0.20376569413619805 11104 3 0.6431721579806045 11104 4 -0.3895823736531412 11104 5 -0.7651092257186605 11104 6 -0.2818627665341893 11104 7 0.16305259820424567 11104 8 -0.354564466237278 11104 9 0.5845801144295137 11104 10-11 -0.18129826309627362 11104 12-13 0.3087439087294044 11104 14-15 -1.357122506968146 11104 16-17 -0.057515313771460796 11104 18-19 -0.8147533424035203 11104 20-21 -0.14146211543702947 11104 22-23 -0.8905369314768947 11104 24-25 0.3398461903383918 11104 26-27 -0.2635237169195932 11104 28-29 -0.958252913437839 11104 30-31 -1.3892149271289753 11104 32-33 -0.6845340099395472 11104 34-35 -0.7065603868821313 11104 36-37 -1.4515218724535899 11104 38-39 0.649268638863731 11104 40-41 -1.5802475985380084 11104 42-43 -1.701423759705719 11104 44-45 -0.3646339354320034 11104 46-47 -0.776072232865701 11104 48-49 -1.2621957648265862 11104 50-51 -0.5592409705773349 11104 52-53 -1.5085349611182899 11104 54-55 -1.3891847482467128 11104 56-57 -0.24731829118776716 11104 58-59 -1.0892107000377322 11104 60-61 -1.440573420648441 11104 62-63 -0.635401520357675 11104 64-65 -0.8752272289747793 11104 66-67 0.8059313036623479 11104 68-69 -0.3523438226245972 11104 70-71 -0.42786343133276716 11104 72-73 0.4110226075974097 11104 74-75 0.19688652126237827 11104 76 0.20102091408551814 11105 1 0.2951722376848771 11105 2 -0.03248364285414951 11105 3 -0.6850329702245226 11105 4 0.35195608788531985 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11105 64-65 1.0865676428200928 11105 66-67 0.4015723293033773 11105 68-69 -0.19542074570151868 11105 70-71 0.8347006712313387 11105 72-73 0.5035867101615139 11105 74-75 0.8273993417751981 11105 76 1.3235850166496235 11106 1 -0.4614045188918787 11106 2 -0.8067094170799223 11106 3 0.43018514499359384 11106 4 -0.16585942993019742 11106 5 -1.6605204811299146 11106 6 0.1408212161497957 11106 7 0.11543355058519467 11106 8 0.1001541484813373 11106 9 -0.6353333054839112 11106 10-11 -0.32960995140796 11106 12-13 0.6663196663051636 11106 14-15 -0.4099363597820016 11106 16-17 -0.16063219688834351 11106 18-19 -0.20566243331261091 11106 20-21 0.35897078499586854 11106 22-23 -0.11226853320849983 11106 24-25 -0.5553919048997074 11106 26-27 -0.2178960112918844 11106 28-29 -0.0940104891954121 11106 30-31 -0.43111968903373565 11106 32-33 -0.17016171556725368 11106 34-35 -0.21651709683883524 11106 36-37 0.2115950106632951 11106 38-39 -0.09592616633106843 11106 40-41 -0.2447497630401756 11106 42-43 0.20316498488303125 11106 44-45 0.028699397901327472 11106 46-47 0.023754606961141178 11106 48-49 -0.8258321284629488 11106 50-51 -1.0867734381098018 11106 52-53 -0.5810890736724019 11106 54-55 0.48259014352817786 11106 56-57 0.08514924127976542 11106 58-59 -0.4513752022022359 11106 60-61 -0.2544262345012527 11106 62-63 0.8420010770449124 11106 64-65 1.0080628143152595 11106 66-67 -0.36324618551513765 11106 68-69 0.6172665669857906 11106 70-71 -0.2505474140167472 11106 72-73 1.4583819149567177 11106 74-75 0.4776657420416015 11106 76 0.7123629054275114 11107 1 -0.4213904788778393 11107 2 -0.16034227071277485 11107 3 0.24046945527790342 11107 4 -0.15462741869818686 11107 5 -0.26546958607902127 11107 6 -2.116637541308961 11107 7 -1.291902356750711 11107 8 0.8277778761050669 11107 9 0.5773729072223048 11107 10-11 0.014107142309129017 11107 12-13 0.021356521342013934 11107 14-15 0.32576037591474005 11107 16-17 0.8981603619042176 11107 18-19 -0.033672261322436725 11107 20-21 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-0.0864869727135229 21610 76 -0.8532762746695006 21611 1 0.5268911360704429 21611 2 0.4470985033946633 21611 3 -0.37164265683420794 21611 4 0.08794849054438814 21611 5 0.9035327495899814 21611 6 0.07171748037939096 21611 7 0.7186081537597957 21611 8 0.4977812127750667 21611 9 -0.6228272929778953 21611 10-11 -0.2771007322320713 21611 12-13 -0.2579227579372656 21611 14-15 0.12188983871086378 21611 16-17 0.03112666153718635 21611 18-19 -0.8863582806751253 21611 20-21 -1.1834374240790098 21611 22-23 -0.6031295240694874 21611 24-25 0.7435498940420899 21611 26-27 -0.0531533465492231 21611 28-29 0.05582116063623488 21611 30-31 0.3986863074389291 21611 32-33 -0.18058339265559198 21611 34-35 -0.6547085350302737 21611 36-37 -0.8258428601079117 21611 38-39 -0.07468197842021596 21611 40-41 0.19135733973359237 21611 42-43 -0.6154978337797914 21611 44-45 0.39906976827169416 21611 46-47 -0.019775936569402575 21611 48-49 -1.110343912974738 21611 50-51 -0.2920804767501721 21611 52-53 -0.32038681297014193 21611 54-55 -0.4585674642960953 21611 56-57 -2.2436145874840676 21611 58-59 -0.5479761321364975 21611 60-61 -0.5393388527472069 21611 62-63 -0.10725337220952724 21611 64-65 -0.7018938956414402 21611 66-67 -0.9975254864611145 21611 68-69 0.5733351897210852 21611 70-71 -0.057986888122890434 21611 72-73 -0.49095270104457 21611 74-75 0.5593556570648488 21611 76 0.06669992643119826 21612 1 1.258899567265864 21612 2 0.7048521920426616 21612 3 1.1504892311513366 21612 4 0.6871655938265349 21612 5 0.07863061167971352 21612 6 0.7841534936284091 21612 7 0.3053290209684718 21612 8 -0.6161904824974371 21612 9 -0.9308670400420311 21612 10-11 -0.5620299704133487 21612 12-13 0.490044721737533 21612 14-15 0.6522270865277946 21612 16-17 0.2834602959846393 21612 18-19 0.12914909662087126 21612 20-21 0.49105820976622283 21612 22-23 0.19474762136863433 21612 24-25 1.0195209870863593 21612 26-27 0.34680148633243846 21612 28-29 0.24190968818817282 21612 30-31 0.8206224712450094 21612 32-33 0.3687993233937874 21612 34-35 0.4710818895406348 21612 36-37 -1.1410340675755393 21612 38-39 0.2214637608149559 21612 40-41 0.23885809251890322 21612 42-43 -0.6857327027951499 21612 44-45 -0.648129870391351 21612 46-47 0.2916513443700808 21612 48-49 0.47073106444170776 21612 50-51 0.612466346495836 21612 52-53 0.24691219335325343 21612 54-55 -0.25268068320605863 21612 56-57 -1.154635364358505 21612 58-59 -0.5691293992247282 21612 60-61 0.15292251431090165 21612 62-63 0.6470375040325678 21612 64-65 -1.0088044647471364 21612 66-67 -0.7927678833295246 21612 68-69 -0.10770967628313599 21612 70-71 1.691486162163173 21612 72-73 -0.021741620044871013 21612 74-75 -0.6943329909327431 21612 76 -1.4537758338819593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 2.0 16 2.0 17 14.0 18 37.0 19 65.0 20 134.0 21 253.0 22 456.0 23 689.0 24 1025.0 25 1349.0 26 1796.0 27 2311.0 28 3045.0 29 3766.0 30 5072.0 31 6916.0 32 9951.0 33 16553.0 34 26109.0 35 27697.0 36 8294.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.833092429265086 16.61891861481603 10.65286445034924 40.89512450556965 2 18.82216490383783 18.007686049128395 42.525144113421156 20.645004933612615 3 17.80035832676978 22.675852757125423 37.90214390195349 21.621645014151312 4 13.409673175460462 35.039295111480406 31.01197895028389 20.53905276277524 5 13.586123925668833 31.419372149181648 42.878904593333736 12.115599331815782 6 23.14403676871543 50.6331524326383 15.881176806626678 10.341633992019595 7 21.738490699948933 26.07476392843851 18.354293429810365 33.83245194180219 8 20.136406519123746 50.772479811662066 13.960030120221228 15.131083548992963 9 20.52069899685815 16.685563931900603 14.354708881137645 48.439028190103606 10-11 20.824678022434565 31.751575266583576 29.286542722614595 18.13720398836726 12-13 21.04304248855345 18.038377316357533 31.722738170456218 29.1958420246328 14-15 19.976717415200326 17.113565351359306 19.26958463522508 43.640132598215295 16-17 32.363658395146146 29.767953123241902 16.298674883370694 21.569713598241254 18-19 33.48884109213186 22.147738141090834 17.479908772628328 26.883511994148986 20-21 21.653669387295846 18.92077862502921 16.523711018980933 42.90184096869401 22-23 20.253772617311412 31.28232203675895 17.122727449766955 31.341177896162687 24-25 32.32743324533691 31.71160254468343 16.754663089107197 19.206301120872464 26-27 33.82848783105882 32.80571678374647 17.471076311791517 15.894719073403191 28-29 21.906365371547782 21.520729548533417 17.748336918462478 38.82456816145633 30-31 43.094620854413805 18.328651989318743 17.588148481556658 20.98857867471079 32-33 24.032252479961222 19.567889479424544 17.588248532797813 38.81160950781643 34-35 18.964196531741877 41.98176286531612 18.377353754538728 20.67668684840328 36-37 18.942849476790986 34.39634056622553 30.530912175320463 16.129897781663015 38-39 19.43966002233051 21.315682422081238 32.57224958238486 26.672407973203388 40-41 18.726332417285178 17.924826347564537 33.123579945037115 30.22526129011317 42-43 31.866220041027237 16.855790120571612 31.985233656184814 19.292756182216337 44-45 20.51074547547539 29.68745942858132 33.271592649973606 16.53020244596969 46-47 30.731179538225174 18.894683314218945 23.137645251336203 27.23649189621968 48-49 30.7122393692064 16.88203778876031 20.084475103213688 32.3212477388196 50-51 20.960384984896614 17.541999532617254 31.994945342184756 29.502670140301372 52-53 17.426884894016638 18.259518595774512 44.594805127361795 19.718791382847055 54-55 16.935054593609756 18.07756057661931 35.38046747536471 29.60691735440622 56-57 29.672745527406807 17.396158806269852 22.898292321940158 30.032803344383186 58-59 18.838961522315845 18.60093738234034 41.80655994529773 20.75354115004609 60-61 29.37587093312099 29.893886806823787 23.40332534166544 17.326916918389777 62-63 18.452337160439868 45.92051793604563 17.47687940070368 18.150265502810825 64-65 17.240245126891253 47.79152096388879 16.570733649551638 18.397500259668316 66-67 17.224083162386506 48.11568988946881 16.3364579817022 18.32376896644249 68-69 18.423535317690437 47.46877623618408 15.88798393588201 18.219704510243474 70-71 29.621679635095248 36.01660074261925 15.723534452166838 18.638185170118664 72-73 47.05411394048505 18.58630359894057 15.605795696504925 18.753786764069453 74-75 49.08511780082054 16.869492963110428 15.253518444786815 18.79187079128222 76 48.95827021786738 16.842524387816045 14.980654208034347 19.21855118628223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 2.5 14 9.5 15 14.0 16 25.0 17 38.0 18 51.0 19 72.5 20 90.0 21 99.0 22 131.0 23 213.5 24 276.0 25 288.0 26 347.5 27 470.5 28 630.5 29 727.0 30 790.5 31 973.0 32 1279.0 33 1466.0 34 1665.5 35 2002.5 36 2280.5 37 2421.0 38 2706.5 39 3220.5 40 3636.0 41 3967.0 42 4111.0 43 4216.5 44 4650.0 45 5030.0 46 5082.0 47 5587.5 48 9132.5 49 18463.0 50 24754.0 51 14525.5 52 3997.0 53 3303.5 54 2910.0 55 2638.5 56 2175.0 57 1884.0 58 1785.0 59 1656.5 60 1434.5 61 1245.5 62 1150.0 63 1029.0 64 836.5 65 687.5 66 582.5 67 555.0 68 466.5 69 371.5 70 356.5 71 348.0 72 328.5 73 255.0 74 211.5 75 222.0 76 190.0 77 153.0 78 135.5 79 123.0 80 107.5 81 89.0 82 80.0 83 74.0 84 60.0 85 41.0 86 32.5 87 29.0 88 23.5 89 14.0 90 8.0 91 5.5 92 5.0 93 8.5 94 8.0 95 11.5 96 19.0 97 30.5 98 76.0 99 2298.5 100 4487.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0025965707955027395 3 0.0 4 8.655235985009131E-4 5 0.0 6 0.0034620943940036524 7 0.0 8 0.0 9 0.0 10-11 8.655235985009131E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0012982853977513698 20-21 0.0 22-23 4.3276179925045655E-4 24-25 0.0017310471970018262 26-27 0.015146662973765981 28-29 0.011684568579762328 30-31 0.006491426988756848 32-33 0.009520759583510044 34-35 0.003029332594753196 36-37 0.0 38-39 0.0 40-41 0.003894856193254109 42-43 0.0034620943940036524 44-45 0.0 46-47 0.003894856193254109 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0012982853977513698 56-57 0.0 58-59 0.002163808996252283 60-61 0.0 62-63 0.0012982853977513698 64-65 0.004327617992504566 66-67 0.0034620943940036524 68-69 0.0 70-71 0.0 72-73 0.0025965707955027395 74-75 0.0025965707955027395 76 0.006924188788007305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 115537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.673836087140916 #Duplication Level Percentage of deduplicated Percentage of total 1 65.9652958054379 20.893739667812042 2 8.637792048094003 5.471840189722773 3 6.23309195245252 5.9227779845417485 4 4.9733570159857905 6.301011797086647 5 4.189096871157262 6.6342383825094995 6 3.139773193059161 5.966919688065295 7 2.2134171334881816 4.907518803500177 8 1.5931138133624814 4.036802063408259 9 0.9974040169422053 2.8432450210754996 >10 1.9702145101789863 8.311623116404268 >50 0.04098920617570706 0.9581346235405108 >100 0.027326137450471376 1.736240338592832 >500 0.0054652274900942755 1.025645464223582 >1k 0.010930454980188551 9.474886832789496 >5k 0.0 0.0 >10k+ 0.0027326137450471378 15.515376026727367 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT 17926 15.515376026727367 Illumina PCR Primer Index 6 (95% over 21bp) GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 4558 3.945056561967162 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC 2697 2.334317145156963 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG 2485 2.150826142274769 No Hit CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT 1207 1.044686983390602 Illumina PCR Primer Index 6 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT 639 0.5530695794420835 Illumina PCR Primer Index 6 (95% over 24bp) CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT 546 0.4725758847814986 Illumina PCR Primer Index 6 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC 395 0.3418818214078607 No Hit CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT 334 0.289084881899305 Illumina PCR Primer Index 6 (95% over 21bp) CTAAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT 230 0.19907042765521 Illumina PCR Primer Index 6 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC 223 0.19301176246570362 Illumina PCR Primer Index 6 (95% over 22bp) TCATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG 207 0.17916338488968903 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT 167 0.1445424409496525 TruSeq Adapter, Index 6 (95% over 23bp) CAAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT 118 0.10213178462310775 Illumina PCR Primer Index 6 (95% over 21bp) TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC 117 0.10126626102460684 Illumina PCR Primer Index 6 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0017310471970018262 0.0 2 0.0 0.0 0.0 0.0017310471970018262 0.0 3 0.0 0.0 0.0 0.0025965707955027395 0.0 4 0.0 0.0 0.0 0.009520759583510044 0.0 5 0.0 0.0 0.0 0.021638089962522828 0.0 6 0.0 0.0 0.0 0.021638089962522828 0.0 7 0.0 0.0 0.0 0.021638089962522828 0.0 8 0.0 0.0 0.0 0.021638089962522828 0.0 9 0.0 0.0 0.0 0.021638089962522828 0.0 10 0.0 0.0 0.0 0.02769675515202922 0.0 11 0.0 0.0 0.0 0.02769675515202922 0.0 12 0.0 0.0 0.0 0.02769675515202922 0.0 13 0.0 0.0 0.0 0.02769675515202922 0.0 14 0.0 0.0 0.0 0.02769675515202922 0.0 15 0.0 0.0 0.0 0.03808303833404018 0.0 16 0.0 0.0 0.0 0.03808303833404018 0.0 17 0.0 0.0 0.0 0.03894856193254109 0.0 18 0.0 0.0 0.0 0.03894856193254109 0.0 19 0.0 0.0 0.0 0.04154513272804383 0.0 20 0.0 0.0 0.0 0.05193141591005479 0.0 21 0.0 0.0 0.0 0.0527969395085557 0.0 22 0.0 0.0 0.0 0.05972112829656301 0.0 23 0.0 0.0 0.0 0.0727039822740767 0.0 24 0.0 0.0 0.0 0.09780416663060318 0.0 25 0.0 0.0 0.0 0.10732492621411323 0.0 26 0.0 0.0 0.0 0.10992149700961597 0.0 27 0.0 0.0 0.0 0.14021482295714793 0.0 28 0.0 0.0 0.0 0.18262547928369266 0.0 29 0.0 0.0 0.0 0.21811194682223012 0.0 30 0.0 0.0 0.0 0.289084881899305 0.0 31 0.0 0.0 0.0 0.3366886798168552 0.0 32 0.0 0.0 0.0 0.41891342167444195 0.0 33 0.0 0.0 0.0 0.5556661502375863 0.0 34 0.0 0.0 0.0 0.6690497416412059 0.0 35 0.0 0.0 0.0 0.8265750365683721 0.0 36 0.0 0.0 0.0 1.1104667768766716 0.0 37 0.0 0.0 0.0 1.6012186572266893 0.0 38 0.0 0.0 0.0 2.0582151172351715 0.0 39 0.0 0.0 0.0 2.690047344140838 0.0 40 0.0 0.0 0.0 3.4049698365025924 0.0 41 0.0 0.0 0.0 4.102581856894329 0.0 42 0.0 0.0 0.0 4.905787756303176 0.0 43 0.0 0.0 0.0 5.674372711771986 0.0 44 0.0 0.0 0.0 6.306204938677653 0.0 45 0.0 0.0 0.0 7.151821494413046 0.0 46 0.0 0.0 0.0 7.910885690298346 0.0 47 0.0 0.0 0.0 8.554835247583025 0.0 48 0.0 0.0 0.0 9.237733366800246 0.0 49 0.0 0.0 0.0 9.960445571548508 0.0 50 0.0 0.0 0.0 10.542077429741122 0.0 51 0.0 0.0 0.0 11.276906964868397 0.0 52 0.0 0.0 0.0 11.953746418896111 0.0 53 0.0 0.0 0.0 12.571730268225764 0.0 54 0.0 0.0 0.0 13.092775474523313 0.0 55 0.0 0.0 0.0 13.720280083436474 0.0 56 0.0 0.0 0.0 14.259501285302544 0.0 57 0.0 0.0 0.0 14.716497745311026 0.0 58 0.0 0.0 0.0 15.229753239222067 0.0 59 0.0 0.0 0.0 15.688480746427551 0.0 60 0.0 0.0 0.0 16.16798081999706 0.0 61 0.0 0.0 0.0 16.55140777413296 0.0 62 0.0 0.0 0.0 16.935700251867367 0.0 63 0.0 0.0 0.0 17.452417840172412 0.0 64 0.0 0.0 0.0 17.95182495650744 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGTC 15 0.0022114988 70.066315 22 CGTCAGC 15 0.0022114988 70.066315 24 ATCTCGG 15 0.0022191168 70.00563 19 CCGATGC 15 0.0022191168 70.00563 33 CCGGAAT 15 0.0022191168 70.00563 3 CGTCTTA 25 2.830755E-6 69.97533 48 GCCGACA 20 7.9123114E-5 69.97533 46 ATGATCT 15 0.002222933 69.97532 41 CGACGTT 15 0.002222933 69.97532 14 ACGTTAC 15 0.002222933 69.97532 16 GCGAACT 15 0.002222933 69.97532 62 TACATGC 15 0.002222933 69.97532 36 GATGCAT 15 0.002222933 69.97532 35 CCGACGT 15 0.002222933 69.97532 13 GTTGGAG 15 0.002222933 69.97532 11 AGGGGGG 2525 0.0 64.43273 70 AGAACGG 35 2.9331204E-7 60.056843 25 TGACGTC 60 1.8189894E-12 58.312767 45 GCCACTA 25 2.3658024E-4 56.077366 31 CACGTAT 25 2.3861646E-4 55.980263 39 >>END_MODULE