FastQCFastQC Report
Wed 25 May 2016
SRR1779990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270736
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3934614.532976774422316No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG35041.2942497488328113No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG4630.17101530642397023No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3930.14515986052833757No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3790.13998877134921106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAG150.00222338869.997413
GCGAAGT200.006947339552.49806238
TCCGAGC200.006947339552.49806265
GCTTCGT200.006947339552.49806265
GTAGACG200.006947339552.4980624
CTGATCG200.006947339552.49806263
CGGGTAG305.872467E-446.6649410
CGTTCAT305.872467E-446.6649458
GCGGGTA305.872467E-446.664949
ACGGAAC305.872467E-446.6649466
GCCGTAT6750.046.14644654
TCGGTGG6750.045.62794545
ATCTCGG6850.044.9618442
TCGCCGT6700.044.92371452
GTCGCCG6950.044.8184951
CGGTGGT6950.044.8184946
GATCTCG6950.044.8184941
TGGTCGC7000.044.49835649
CGCCGTA6850.044.45091253
CTCGGTG6950.044.3149144