FastQCFastQC Report
Wed 25 May 2016
SRR1779990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270736
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT216117.982314875007387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62082.293008687429821No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC36451.3463300041368713No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG26920.9943265764434726No Hit
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT17100.6316116068790261No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT7980.2947520832102121No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT7200.26594172921222153RNA PCR Primer, Index 37 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC5530.20425802257549788No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4340.16030376455292242No Hit
CTAAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT3370.12447550381183145No Hit
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC2980.11007032681283613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT501.4551915E-1162.99085644
AGGGGGG33550.062.3869770
ATCGTAT453.8380676E-1062.21319239
AGCCGTC553.6379788E-1157.26441245
ATATCGT653.6379788E-1253.83833737
TCGAATG653.6379788E-1253.83833740
GAACGAG200.00694523752.5020869
GCCGTAA200.00694523752.5020848
GGTCGCG200.00695031352.4923826
CAATACG200.00695031352.49238217
CGAATGC608.367351E-1152.4923841
AAGGGGG39800.052.2382569
GCCGTCT39950.051.68210646
CGTCTTC39700.051.66451348
CCGTCTT39650.051.6318547
TCTTCTG39350.050.96771250
GTCTTCT39200.050.89486749
TATGCCG41550.050.53418443
TGCCGTC41450.050.48724445
CGTATGC41850.050.42278741