FastQCFastQC Report
Wed 25 May 2016
SRR1779989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76336
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3671748.09919304129113No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG34004.453992873611402No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG4220.5528191154894152No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3870.5069691888492979No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3650.478149234961224No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1010.1323097883043387No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAA150.002216930570.029
TTGTGCG207.8813464E-570.02
ATCAGCG150.002216930570.02
GTGATTA150.002216930570.04
AGAAATA150.002216930570.049
TCGGAGA150.002216930570.06
TGCTCAA150.002216930570.030
CGACTGT150.002216930570.041
TGTGCGC252.3768672E-456.0000043
GCGTCTG252.3768672E-456.0000048
AAAAAGG3750.053.270
GTTGATC200.00692731252.529
TCCATTG200.00692731252.55
ACCGCCA200.00692731252.59
CATTGGT200.00692731252.57
TGGAAAA200.00692731252.512
TGGCGCT200.00692731252.513
AAGAAAT200.00692731252.532
GAATAAA200.00692731252.51
TGCATTC200.00692731252.57
Produced by FastQC (version 0.11.5)