##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779989_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76336 Sequences flagged as poor quality 0 Sequence length 76 %GC 75 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.466319953888075 32.0 27.0 32.0 27.0 32.0 2 30.076949276881155 32.0 32.0 32.0 27.0 32.0 3 30.009929784112344 32.0 32.0 32.0 27.0 32.0 4 29.44947338084259 32.0 32.0 32.0 22.0 32.0 5 30.00340599455041 32.0 32.0 32.0 27.0 32.0 6 34.31416369733808 37.0 37.0 37.0 27.0 37.0 7 34.04603332634668 37.0 37.0 37.0 27.0 37.0 8 34.1943381890589 37.0 37.0 37.0 27.0 37.0 9 34.410867742611615 37.0 37.0 37.0 27.0 37.0 10-11 34.182096258645984 37.0 37.0 37.0 27.0 37.0 12-13 34.04110773422762 37.0 37.0 37.0 27.0 37.0 14-15 34.387379480192834 37.0 37.0 37.0 27.0 37.0 16-17 34.60578888073779 37.0 37.0 37.0 29.5 37.0 18-19 34.248552452316076 37.0 37.0 37.0 27.0 37.0 20-21 34.56380344791448 37.0 37.0 37.0 29.5 37.0 22-23 34.65686569901489 37.0 37.0 37.0 29.5 37.0 24-25 34.27297736323622 37.0 37.0 37.0 27.0 37.0 26-27 34.552635715782856 37.0 37.0 37.0 29.5 37.0 28-29 34.42367297212324 37.0 37.0 37.0 27.0 37.0 30-31 34.01346677845316 37.0 37.0 37.0 24.5 37.0 32-33 34.30127986795221 37.0 37.0 37.0 27.0 37.0 34-35 34.326602127436594 37.0 37.0 37.0 27.0 37.0 36-37 34.88250628798994 37.0 37.0 37.0 29.5 37.0 38-39 34.97053814713897 37.0 37.0 37.0 32.0 37.0 40-41 34.57225948438483 37.0 37.0 37.0 29.5 37.0 42-43 34.48946106686229 37.0 37.0 37.0 27.0 37.0 44-45 34.361769021169565 37.0 37.0 37.0 27.0 37.0 46-47 34.54880397191364 37.0 37.0 37.0 29.5 37.0 48-49 34.9243803709914 37.0 37.0 37.0 32.0 37.0 50-51 35.08159976944037 37.0 37.0 37.0 32.0 37.0 52-53 34.46247511003982 37.0 37.0 37.0 27.0 37.0 54-55 34.526114808216306 37.0 37.0 37.0 29.5 37.0 56-57 34.409000995598404 37.0 37.0 37.0 27.0 37.0 58-59 34.178415164535735 37.0 37.0 37.0 27.0 37.0 60-61 33.99777955355272 37.0 37.0 37.0 24.5 37.0 62-63 33.765575875078596 37.0 37.0 37.0 22.0 37.0 64-65 33.566593743450014 37.0 37.0 37.0 22.0 37.0 66-67 33.67438692098092 37.0 37.0 37.0 22.0 37.0 68-69 33.90837219660449 37.0 37.0 37.0 22.0 37.0 70-71 33.926653217354854 37.0 37.0 37.0 22.0 37.0 72-73 33.954222123244605 37.0 37.0 37.0 24.5 37.0 74-75 33.6252619995808 37.0 37.0 37.0 17.5 37.0 76 33.783051247118 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -3.2773087674916432 11101 2 -0.5025718151254068 11101 3 -1.1823689320553967 11101 4 -1.8217300459800327 11101 5 -3.455252059308311 11101 6 -2.187595932279919 11101 7 -1.9158685899475287 11101 8 -2.791026571909157 11101 9 -1.7998367617377937 11101 10-11 -2.6371821720608395 11101 12-13 -2.4754445596399535 11101 14-15 -0.5730057415536152 11101 16-17 -0.1836088729750358 11101 18-19 -2.5527251772855664 11101 20-21 -1.3615018297841246 11101 22-23 -0.6450171638219615 11101 24-25 -1.7596649227665608 11101 26-27 0.7766326723431405 11101 28-29 -1.2763374093167883 11101 30-31 0.2585509166790203 11101 32-33 -0.8701041108764898 11101 34-35 -1.988192014594766 11101 36-37 -0.525693472792959 11101 38-39 -0.45667849874611477 11101 40-41 -0.309864490573581 11101 42-43 -1.1190735700861296 11101 44-45 -0.5355567560210162 11101 46-47 -1.6687384889837205 11101 48-49 -0.5465069732808772 11101 50-51 0.15544756896021283 11101 52-53 -2.811595334581206 11101 54-55 -1.4930241175434418 11101 56-57 -1.191129834333566 11101 58-59 -1.3763737692789775 11101 60-61 -0.3902321750869575 11101 62-63 -1.9992006561845947 11101 64-65 -2.1792863066534522 11101 66-67 -1.3283615197508851 11101 68-69 -0.7746850723617271 11101 70-71 -1.2953753498641731 11101 72-73 -2.0195025716424198 11101 74-75 -1.3547449396555962 11101 76 0.3903029415991597 11102 1 0.3074780542137816 11102 2 1.1500444639443614 11102 3 1.2047434710453757 11102 4 1.133696310609114 11102 5 0.8848642197614573 11102 6 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1.2450538929258173 11105 68-69 1.2930303859146122 11105 70-71 0.8076798256944215 11105 72-73 0.5974604926530702 11105 74-75 0.7980138382741728 11105 76 0.735948176437283 11106 1 0.2687875780233071 11106 2 -0.6981698217699233 11106 3 -0.47680414800224113 11106 4 -0.21749416558136048 11106 5 0.0277213626185997 11106 6 1.0121548982848694 11106 7 -0.5288254005787536 11106 8 -0.011126239683243 11106 9 0.11004695919243801 11106 10-11 0.3904341070089288 11106 12-13 -0.5300541941914503 11106 14-15 -0.21814693756690673 11106 16-17 0.0728280041013818 11106 18-19 -0.40384643974403645 11106 20-21 0.8507074725414512 11106 22-23 0.49161074315478004 11106 24-25 0.6234330838779556 11106 26-27 0.17779546304081606 11106 28-29 -0.20428591430017917 11106 30-31 0.25190639840659657 11106 32-33 -0.15311906104260942 11106 34-35 0.28444087577068444 11106 36-37 -0.6467482900687074 11106 38-39 -0.9905240136962732 11106 40-41 -0.44150900884600475 11106 42-43 -0.484937357461547 11106 44-45 -0.9458557593432673 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24-25 0.4827659679749914 21611 26-27 1.1919464064370473 21611 28-29 0.8366507433817603 21611 30-31 0.3678093633661632 21611 32-33 0.2924671923266615 21611 34-35 0.25226432590545755 21611 36-37 0.47606168297711093 21611 38-39 0.014361431047660744 21611 40-41 0.4126015032833692 21611 42-43 0.9217338015681094 21611 44-45 0.16869949672142326 21611 46-47 0.43876480193334544 21611 48-49 0.37165888893940036 21611 50-51 1.1550087800176954 21611 52-53 0.30424494624372045 21611 54-55 0.6114076507759947 21611 56-57 0.25840943727679644 21611 58-59 0.19931732330549323 21611 60-61 -0.2496003190097298 21611 62-63 -1.0090734073912628 21611 64-65 0.7658650755317211 21611 66-67 0.01937871719514561 21611 68-69 0.1770481439612226 21611 70-71 0.03612969620866835 21611 72-73 0.519769038713001 21611 74-75 1.0789979300240873 21611 76 0.04190452123935273 21612 1 0.6199780542137816 21612 2 0.7542111306110293 21612 3 0.12141013771204356 21612 4 -1.4496370227242181 21612 5 0.5098642197614573 21612 6 0.2562025173324898 21612 7 1.1824841232307648 21612 8 0.536492807935808 21612 9 0.08921362585910231 21612 10-11 0.3487674403422574 21612 12-13 0.21399342485616302 21612 14-15 -0.7999921756621475 21612 16-17 0.09812562314899509 21612 18-19 0.38186784597024825 21612 20-21 0.15279080587477978 21612 22-23 -0.018805923511891365 21612 24-25 -0.5581145351696577 21612 26-27 0.07362879637415176 21612 28-29 0.5412497999855361 21612 30-31 -1.2227959825457901 21612 32-33 -0.2959762038997482 21612 34-35 0.7591432567230711 21612 36-37 0.39194218612176 21612 38-39 0.9127497958275299 21612 40-41 -0.36412805646504864 21612 42-43 0.20553883301464282 21612 44-45 0.10325138351387153 21612 46-47 0.4218622862100716 21612 48-49 0.4031054298198953 21612 50-51 -0.24062801243513832 21612 52-53 -0.13305851287578463 21612 54-55 -0.5187024121170936 21612 56-57 -0.3752383614653496 21612 58-59 -0.2147943119146305 21612 60-61 0.7238666621223473 21612 62-63 0.35472376242005765 21612 64-65 1.1758493522612739 21612 66-67 0.3167450693963971 21612 68-69 0.8861676408165735 21612 70-71 -0.013398605678126785 21612 72-73 0.8719702965746308 21612 74-75 -0.9538243970199431 21612 76 -0.5103753529744779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 17.0 13 28.0 14 64.0 15 123.0 16 174.0 17 256.0 18 334.0 19 363.0 20 372.0 21 460.0 22 588.0 23 658.0 24 798.0 25 988.0 26 1016.0 27 1153.0 28 1363.0 29 1513.0 30 1761.0 31 2186.0 32 2843.0 33 4021.0 34 7305.0 35 14233.0 36 33715.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 69.922448124083 8.340756654789352 4.894152169356529 16.842643051771116 2 60.5101131838189 9.24203521274366 19.496698805281913 10.751152798155523 3 59.53285474743241 9.653374554600713 18.954359673024523 11.859411024942359 4 58.01532717626253 12.853867819479925 15.411017226698107 13.719787777559441 5 57.86129742192413 14.865856214630057 20.15169775728359 7.1211486061622296 6 63.86192441213075 19.917469050894084 8.575358616624092 7.645247920351084 7 62.68208970865646 14.003877593795849 8.889645776566757 14.424386920980927 8 62.54977992035212 19.656518549570322 8.960385663382938 8.833315866694614 9 61.57383148186963 8.738996017606372 8.41018654370153 21.276985956822468 10-11 63.1235590023056 13.41765353175435 13.168753929993713 10.290033535946343 12-13 61.80963110459024 10.662727939635296 13.665898134562985 13.861742821211486 14-15 65.39771536365542 12.075560679102914 10.076503877593796 12.450220079647872 16-17 65.00317676572499 11.556877206542172 9.819808608052611 13.620137419680228 18-19 62.005881929115546 10.815413536297005 13.242200548892717 13.93650398569473 20-21 64.88304610688623 10.66504221606503 10.853688092384079 13.598223584664662 22-23 65.1933556906309 10.63325298679522 9.400544959128066 14.772846363445819 24-25 62.69077094386586 14.079386912949499 9.464203838344142 13.765638304840506 26-27 64.76148056932882 13.905718833308226 10.06346981417558 11.269330783187376 28-29 62.49230366149211 14.582432697976028 12.73662147114692 10.188642169384948 30-31 65.02456278247199 14.197943276347678 10.848234754699678 9.929259186480644 32-33 65.46709810959874 14.037834226350334 10.029083096432736 10.465984567618198 34-35 65.48284196529748 14.044108496158355 10.531935101428582 9.94111443711559 36-37 65.5575133131153 10.808350090719138 13.410712063352742 10.223424532812817 38-39 65.25316041134474 10.594091832056069 14.123272417632805 10.029475338966398 40-41 64.72611039424645 12.608812413621449 11.88372382443293 10.78135336769917 42-43 61.929246549770426 13.135435020403353 14.350466034806871 10.584852395019356 44-45 61.27096829129304 10.312371046236679 14.920973858820602 13.49568680364968 46-47 63.68793025525476 9.821773617779408 12.760773165827171 13.729522961138658 48-49 64.76105638230979 9.963189058897507 14.717826451477677 10.557928107315028 50-51 66.73697034800323 10.32219609487067 12.473226742472376 10.467606814653733 52-53 61.68362033392065 10.388351422339541 14.172960156152772 13.755068087587032 54-55 63.2608681412973 10.631357625220245 11.98197431077284 14.125799922709618 56-57 63.21519103419817 10.92821725432144 11.637595058590808 14.218996652889585 58-59 61.003071965206225 13.50354684255687 14.193920259905287 11.299460932331614 60-61 60.167421021949444 11.744361985733843 14.622483935835042 13.46573305648167 62-63 60.54077776103859 13.827954594580433 13.706122395509238 11.925145248871742 64-65 60.69758302220476 14.050566581515685 14.303399489094124 10.948450907185432 66-67 60.95016015039072 17.370913544989484 10.398312689544047 11.280613615075751 68-69 61.2408381421488 18.17044494370247 9.69208297581073 10.896633938337995 70-71 61.13880355540417 18.082256384727742 9.664699447825033 11.114240612043048 72-73 62.120825063700735 18.00127073958354 9.412643203835799 10.465260992879928 74-75 64.4968166216889 15.910996410511698 9.591400948463333 10.000786019336076 76 68.94478286500295 11.944717364249689 9.428178424051877 9.682321346695486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 2.0 10 3.0 11 5.0 12 6.0 13 11.5 14 12.5 15 8.5 16 17.5 17 26.0 18 28.0 19 26.0 20 39.0 21 56.0 22 80.0 23 138.0 24 156.5 25 141.0 26 188.0 27 235.5 28 302.5 29 369.0 30 340.0 31 406.0 32 528.5 33 556.0 34 575.5 35 703.0 36 844.5 37 878.0 38 944.0 39 1086.5 40 1216.0 41 1369.0 42 1469.0 43 2266.0 44 3616.5 45 2862.0 46 1554.0 47 1592.0 48 1543.0 49 1382.0 50 1308.0 51 1287.0 52 1314.5 53 1230.5 54 1098.0 55 1140.5 56 1113.5 57 1030.5 58 1017.0 59 885.0 60 780.5 61 764.5 62 721.0 63 663.0 64 550.0 65 460.0 66 414.5 67 404.0 68 381.5 69 349.0 70 295.0 71 251.0 72 241.5 73 207.5 74 196.0 75 209.0 76 188.0 77 140.5 78 114.5 79 115.0 80 117.5 81 115.0 82 96.5 83 83.0 84 86.5 85 78.0 86 76.0 87 86.0 88 88.0 89 87.0 90 87.5 91 112.0 92 133.0 93 143.5 94 174.5 95 228.5 96 262.0 97 330.5 98 633.0 99 19019.5 100 37172.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0013099979040033535 5 0.0 6 0.0013099979040033535 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.549989520016768E-4 18-19 6.549989520016768E-4 20-21 0.0032749947600083837 22-23 0.0 24-25 0.0013099979040033535 26-27 6.549989520016768E-4 28-29 0.0013099979040033535 30-31 0.0013099979040033535 32-33 0.003929993712010061 34-35 0.0019649968560050304 36-37 0.0019649968560050304 38-39 0.0013099979040033535 40-41 6.549989520016768E-4 42-43 6.549989520016768E-4 44-45 6.549989520016768E-4 46-47 6.549989520016768E-4 48-49 0.0 50-51 6.549989520016768E-4 52-53 6.549989520016768E-4 54-55 6.549989520016768E-4 56-57 0.0019649968560050304 58-59 6.549989520016768E-4 60-61 0.0019649968560050304 62-63 0.0019649968560050304 64-65 0.0013099979040033535 66-67 0.0019649968560050304 68-69 6.549989520016768E-4 70-71 0.0019649968560050304 72-73 0.0032749947600083837 74-75 0.002619995808006707 76 0.0013099979040033535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 76336.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.808006707189268 #Duplication Level Percentage of deduplicated Percentage of total 1 68.16293125157391 14.18334730664431 2 6.975572903550743 2.9029553552714313 3 5.49609670108285 3.4308845105847827 4 5.105766809367918 4.249633200586879 5 3.9473684210526314 4.106843429050514 6 3.9284814908083607 4.904632152588556 7 2.260135985897759 3.2920247327604275 8 1.4291110551498363 2.3789561936700903 9 1.007302946361118 1.886396981764829 >10 1.63057164442206 4.203783273946762 >50 0.018886930244270964 0.23710962062460703 >100 0.02518257365902795 1.6702473276042757 >500 0.0 0.0 >1k 0.0062956434147569875 4.453992873611402 >5k 0.0 0.0 >10k+ 0.0062956434147569875 48.09919304129113 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 36717 48.09919304129113 No Hit CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG 3400 4.453992873611402 No Hit CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG 422 0.5528191154894152 No Hit TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT 387 0.5069691888492979 No Hit ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC 365 0.478149234961224 No Hit TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG 101 0.1323097883043387 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0170299727520436 0.0 5 0.0 0.0 0.0 0.03536994340809055 0.0 6 0.0 0.0 0.0 0.03536994340809055 0.0 7 0.0 0.0 0.0 0.03536994340809055 0.0 8 0.0 0.0 0.0 0.03536994340809055 0.0 9 0.0 0.0 0.0 0.0366799413120939 0.0 10 0.0 0.0 0.0 0.05239991616013415 0.0 11 0.0 0.0 0.0 0.0537099140641375 0.0 12 0.0 0.0 0.0 0.0537099140641375 0.0 13 0.0 0.0 0.0 0.06156990148815762 0.0 14 0.0 0.0 0.0 0.0759798784321945 0.0 15 0.0 0.0 0.0 0.0759798784321945 0.0 16 0.0 0.0 0.0 0.08383986585621463 0.0 17 0.0 0.0 0.0 0.08383986585621463 0.0 18 0.0 0.0 0.0 0.08383986585621463 0.0 19 0.0 0.0 0.0 0.10348983441626493 0.0 20 0.0 0.0 0.0 0.10348983441626493 0.0 21 0.0 0.0 0.0 0.11658981345629847 0.0 22 0.0 0.0 0.0 0.19125969398448964 0.0 23 0.0 0.0 0.0 0.20435967302452315 0.0 24 0.0 0.0 0.0 0.23055963110459024 0.0 25 0.0 0.0 0.0 0.2606895828966674 0.0 26 0.0 0.0 0.0 0.2698595682246908 0.0 27 0.0 0.0 0.0 0.2868895409767344 0.0 28 0.0 0.0 0.0 0.3026095158247747 0.0 29 0.0 0.0 0.0 0.3379794592328652 0.0 30 0.0 0.0 0.0 0.3602494236009222 0.0 31 0.0 0.0 0.0 0.3772793963529658 0.0 32 0.0 0.0 0.0 0.43360930622511 0.0 33 0.0 0.0 0.0 0.5423391322573884 0.0 34 0.0 0.0 0.0 0.6576189478096836 0.0 35 0.0 0.0 0.0 0.8213686858101027 0.0 36 0.0 0.0 0.0 1.1200482079228673 0.0 37 0.0 0.0 0.0 1.5012575979878433 0.0 38 0.0 0.0 0.0 1.8732970027247957 0.0 39 0.0 0.0 0.0 2.316076294277929 0.0 40 0.0 0.0 0.0 2.9396352965835253 0.0 41 0.0 0.0 0.0 3.559264305177112 0.0 42 0.0 0.0 0.0 4.023003563194299 0.0 43 0.0 0.0 0.0 4.5312827499476 0.0 44 0.0 0.0 0.0 5.1417417732131625 0.0 45 0.0 0.0 0.0 5.770540767134772 0.0 46 0.0 0.0 0.0 6.225110039823936 0.0 47 0.0 0.0 0.0 6.829019073569483 0.0 48 0.0 0.0 0.0 7.36349821840285 0.0 49 0.0 0.0 0.0 7.903217354852233 0.0 50 0.0 0.0 0.0 8.427216516453573 0.0 51 0.0 0.0 0.0 8.883095787046742 0.0 52 0.0 0.0 0.0 9.28919513728778 0.0 53 0.0 0.0 0.0 9.832844267449172 0.0 54 0.0 0.0 0.0 10.181303709914063 0.0 55 0.0 0.0 0.0 10.646352965835254 0.0 56 0.0 0.0 0.0 11.010532383148186 0.0 57 0.0 0.0 0.0 11.4454516872773 0.0 58 0.0 0.0 0.0 11.817491092014253 0.0 59 0.0 0.0 0.0 12.124030601551038 0.0 60 0.0 0.0 0.0 12.451530077551876 0.0 61 0.0 0.0 0.0 12.678159714944456 0.0 62 0.0 0.0 0.0 12.915269335569063 0.0 63 0.0 0.0 0.0 13.19560888702578 0.0 64 0.0 0.0 0.0 13.62921819325089 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCAAA 15 0.0022169305 70.0 29 TTGTGCG 20 7.8813464E-5 70.0 2 ATCAGCG 15 0.0022169305 70.0 2 GTGATTA 15 0.0022169305 70.0 4 AGAAATA 15 0.0022169305 70.0 49 TCGGAGA 15 0.0022169305 70.0 6 TGCTCAA 15 0.0022169305 70.0 30 CGACTGT 15 0.0022169305 70.0 41 TGTGCGC 25 2.3768672E-4 56.000004 3 GCGTCTG 25 2.3768672E-4 56.000004 8 AAAAAGG 375 0.0 53.2 70 GTTGATC 20 0.006927312 52.5 29 TCCATTG 20 0.006927312 52.5 5 ACCGCCA 20 0.006927312 52.5 9 CATTGGT 20 0.006927312 52.5 7 TGGAAAA 20 0.006927312 52.5 12 TGGCGCT 20 0.006927312 52.5 13 AAGAAAT 20 0.006927312 52.5 32 GAATAAA 20 0.006927312 52.5 1 TGCATTC 20 0.006927312 52.5 7 >>END_MODULE