FastQCFastQC Report
Wed 25 May 2016
SRR1779989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76336
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT1962325.70608887025781No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56137.353018235170823No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC29153.8186438901697755No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG25513.3418046531125554No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT13431.759327185076504No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT7130.9340285055543912TruSeq Adapter, Index 7 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT6100.7990987214420456No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT4870.6379689792496331No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC4160.544959128065395No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3840.5030391951372878No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT2910.3812093900649759No Hit
CTAAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2680.3510794382728988No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC2600.3405994550408719No Hit
TCATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG2200.2881995388807378No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT1830.2397296164326137No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT1390.18208970865646615No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG1180.1545797526723957No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT1130.14802976315237895No Hit
CTCTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC960.12575979878432195No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCC960.12575979878432195No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC900.11789981136030181No Hit
CAAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT850.11134982184028505No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT830.10872982603227835No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC800.10479983232026828No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG800.10479983232026828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC150.002216930570.051
ACAACGA150.002216930570.019
ACAGTTT150.002216930570.037
CGAACAA150.002216930570.032
ATGTGGT150.002216930570.056
GTCTACA150.002216930570.049
TGAGTAA150.002216930570.034
TGGTTAT150.002216930570.018
TCTACAG150.002216930570.050
AATGACG150.002216930570.043
CGAGGCT150.002216930570.040
ACTACGA150.002216930570.051
ACTACAG150.002216930570.02
CGAATGA150.002216930570.041
ACGTCAT207.8813464E-570.047
TACTACG150.002216930570.050
ACCAATG207.8813464E-570.066
CCGCGTT150.002216930570.030
ATTATTA150.002216930570.012
CGCGTTG150.002216930570.031
Produced by FastQC (version 0.11.5)