FastQCFastQC Report
Wed 25 May 2016
SRR1779988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67287
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3877857.63074590931383No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG45306.732355432698739No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG5100.7579473003700566No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT4920.7311962191805252No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC4020.5974408132328681No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1230.1827990547951313No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880.13078306359326466No Hit
CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG810.12037986535289136No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAT150.002213161270.02083634
CGGAGGT150.002213161270.02083643
TACAGTA150.002213161270.02083633
ACAGAGT150.002213161270.02083615
TTGTATT150.002213161270.02083641
GGTTTAG150.002213161270.02083646
TTAGGGC150.002213161270.02083649
GAAGACT207.862911E-570.0208341
GTCATCA150.002219706369.968787
CAGCAAA150.002219706369.9687812
AATTTAT150.002219706369.9687827
TCAGGCG150.002219706369.9687822
GGCAAGG150.002219706369.9687828
ACTCTAT150.002219706369.9687825
TAGTCAT150.002219706369.968785
CTGAATT150.002219706369.9687824
TCAGCAA150.002219706369.9687811
TGTAGTC150.002219706369.968783
TCATCAG150.002219706369.968788
TCTTTAT301.0128133E-769.968781
Produced by FastQC (version 0.11.5)