FastQCFastQC Report
Wed 25 May 2016
SRR1779988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67287
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT2230533.149048107361004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG735410.929302837100776No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC32084.7676371364453765No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG30084.470402901006138No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT16142.39868027999465No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT8251.2260912211868562No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT5860.870896309836967RNA PCR Primer, Index 36 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC4530.6732355432698739No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3900.5796067591065139No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3200.4755747767027806No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC2670.39680770431138257No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT2600.3864045060710093No Hit
TCATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG2420.35965342488147783No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG1730.2571076136549408No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG1400.2080639648074665No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC1270.18874373950391604No Hit
CAAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT1180.17536819890915037No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT1050.15604797360559988No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC990.14713094654242276No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT950.141186261833638No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC950.141186261833638No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC890.13226923477046088No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAACCCATCTCGTATGCCGT810.12037986535289136No Hit
CATAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT750.1114628382897142No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG730.10849049593532183No Hit
CTTATACAAATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT700.10403198240373325No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA690.10254581122653708No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAG150.00221222970.0282818
CTTTACA150.00221222970.028281
AATTTTG150.00221222970.0282816
AGCTAGA150.00221222970.0282855
GACGGAA301.006847E-770.0282826
AGTGGAT150.00221222970.0282826
TGCTGAT150.00221222970.0282830
CTGATGA150.00221222970.0282832
CGGAAAA301.006847E-770.0282828
GCTGATG150.00221222970.0282831
TCGTCAA150.00221222970.0282817
TTTGTAA150.00221222970.0282819
TTGCTGA150.00221222970.0282829
ACTGCTT207.858773E-570.02827553
GAAAACC252.8052364E-670.02827530
GTACACC207.858773E-570.02827530
ACCGAGC252.8052364E-670.02827513
CATTATA207.858773E-570.0282752
AAAAGCG207.858773E-570.02827567
TACTGCT252.8052364E-670.02827552
Produced by FastQC (version 0.11.5)