##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779988_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67287 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.330390714402483 32.0 27.0 32.0 22.0 32.0 2 30.274986252916612 32.0 32.0 32.0 27.0 32.0 3 28.20696419813634 32.0 27.0 32.0 13.0 32.0 4 30.995868444127396 32.0 32.0 32.0 27.0 32.0 5 28.353411505937252 32.0 27.0 32.0 13.0 32.0 6 34.943971346619705 37.0 37.0 37.0 32.0 37.0 7 34.46616731315113 37.0 37.0 37.0 27.0 37.0 8 34.088293429637226 37.0 32.0 37.0 27.0 37.0 9 34.05862945294039 37.0 37.0 37.0 27.0 37.0 10-11 35.04088456908467 37.0 37.0 37.0 29.5 37.0 12-13 34.92399720599819 37.0 37.0 37.0 29.5 37.0 14-15 35.00853805341299 37.0 37.0 37.0 27.0 37.0 16-17 32.74141364602375 37.0 32.0 37.0 20.0 37.0 18-19 33.677590024819054 37.0 37.0 37.0 24.5 37.0 20-21 34.78261774191151 37.0 37.0 37.0 29.5 37.0 22-23 34.35953453118731 37.0 34.5 37.0 29.5 37.0 24-25 34.38817304977188 37.0 37.0 37.0 27.0 37.0 26-27 34.800422072614325 37.0 37.0 37.0 29.5 37.0 28-29 34.68424064083701 37.0 37.0 37.0 29.5 37.0 30-31 34.662988393003104 37.0 37.0 37.0 29.5 37.0 32-33 34.7041256111879 37.0 37.0 37.0 29.5 37.0 34-35 33.61455407433829 37.0 34.5 37.0 24.5 37.0 36-37 32.601104225184656 37.0 32.0 37.0 13.0 37.0 38-39 32.99330479884674 37.0 32.0 37.0 22.0 37.0 40-41 33.80187108951209 37.0 34.5 37.0 24.5 37.0 42-43 34.09466167313151 37.0 37.0 37.0 24.5 37.0 44-45 34.49702022678972 37.0 37.0 37.0 27.0 37.0 46-47 33.91886991543686 37.0 34.5 37.0 27.0 37.0 48-49 33.02834128434913 37.0 34.5 37.0 17.5 37.0 50-51 33.54713391888478 37.0 34.5 37.0 22.0 37.0 52-53 33.07957703568297 37.0 34.5 37.0 22.0 37.0 54-55 33.15812861325368 37.0 32.0 37.0 22.0 37.0 56-57 33.01981066179202 37.0 34.5 37.0 17.5 37.0 58-59 33.106744244802115 37.0 32.0 37.0 22.0 37.0 60-61 33.30009511495534 37.0 34.5 37.0 17.5 37.0 62-63 34.01891895908571 37.0 37.0 37.0 27.0 37.0 64-65 34.190378527798835 37.0 37.0 37.0 27.0 37.0 66-67 34.096118120885166 37.0 37.0 37.0 27.0 37.0 68-69 33.46873838928768 37.0 34.5 37.0 22.0 37.0 70-71 31.036730720644403 37.0 29.5 37.0 13.0 37.0 72-73 32.63751541902596 37.0 34.5 37.0 13.0 37.0 74-75 33.754863495177375 37.0 37.0 37.0 22.0 37.0 76 33.86093896294975 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.7628399725363728 11101 2 -0.11211332963507559 11101 3 0.3421634168741683 11101 4 -0.8149896985275085 11101 5 -1.7995346210415413 11101 6 -0.7857301383017443 11101 7 -2.2481797962447416 11101 8 -0.8278702142125738 11101 9 1.0772286367175639 11101 10-11 -1.3235277684631868 11101 12-13 -0.4385983328364418 11101 14-15 0.878084237931489 11101 16-17 -1.0381341443594394 11101 18-19 -0.3411773795989319 11101 20-21 0.04162647415404308 11101 22-23 -0.8463131771841077 11101 24-25 -1.3559519855075592 11101 26-27 -0.6565162705483232 11101 28-29 -0.28459142342980925 11101 30-31 -1.5606183357330679 11101 32-33 -0.15548276159992014 11101 34-35 -0.29457158473664435 11101 36-37 -1.5053554364572577 11101 38-39 -0.32103550006748094 11101 40-41 -0.687931990759111 11101 42-43 0.8445911467634915 11101 44-45 -1.4769402109112377 11101 46-47 0.4130795015167905 11101 48-49 -0.3430199077433187 11101 50-51 -1.6175144336939802 11101 52-53 -0.8562845901718461 11101 54-55 -2.8046090814133464 11101 56-57 -0.5033457862498167 11101 58-59 -0.8394365745156094 11101 60-61 -0.6545666262186529 11101 62-63 -0.4664288962287344 11101 64-65 -0.7663468450260709 11101 66-67 -0.5304220434714182 11101 68-69 -1.6653509197476097 11101 70-71 -1.380246209285776 11101 72-73 0.13289073898967274 11101 74-75 -0.9558276356151438 11101 76 -0.8420832314256828 11102 1 0.7783955280919272 11102 2 0.8301088925871483 11102 3 -0.375614360903608 11102 4 0.629454745916938 11102 5 0.278243156736238 11102 6 0.8476031950315885 11102 7 0.26070909264414155 11102 8 -0.7678702142125715 11102 9 -1.9472158077268773 11102 10-11 -0.556861101796521 11102 12-13 -0.05415388839199409 11102 14-15 -0.0719157620685138 11102 16-17 -0.06924525547054827 11102 18-19 -0.5611773795989308 11102 20-21 0.4938486963762685 11102 22-23 0.7959090450381154 11102 24-25 -0.7970630966186647 11102 26-27 -0.24429404832611112 11102 28-29 -0.35792475676313273 11102 30-31 -0.19617389128862328 11102 32-33 0.20340612728896446 11102 34-35 0.20876174859669305 11102 36-37 -0.6042443253461443 11102 38-39 -2.3377021667341538 11102 40-41 -0.7679319907591164 11102 42-43 0.7057022578745986 11102 44-45 -0.5069402109112389 11102 46-47 0.10530172373901081 11102 48-49 -0.38968657440998555 11102 50-51 -1.0675144336939795 11102 52-53 -1.055173479060734 11102 54-55 -0.5168313036355698 11102 56-57 -0.41445689736092817 11102 58-59 -0.3149921300711611 11102 60-61 0.24210004044801536 11102 62-63 -1.5442066740065172 11102 64-65 -0.6319024005816232 11102 66-67 0.586244623195256 11102 68-69 0.5435379691412763 11102 70-71 -0.8691350981746631 11102 72-73 0.03066851676744875 11102 74-75 1.555283475495969 11102 76 0.9312501019076507 11103 1 -1.6149378052414036 11103 2 0.0878866703649237 11103 3 -1.0578365831258303 11103 4 -0.37054525408306205 11103 5 -0.15509017659709556 11103 6 0.8364920839204757 11103 7 -0.04817979624474589 11103 8 0.7054631191207577 11103 9 0.41056197005089956 11103 10-11 -0.023527768463189602 11103 12-13 0.09473500049688965 11103 14-15 -0.24413798429074518 11103 16-17 0.35075474452945343 11103 18-19 -0.6522884907100419 11103 20-21 -0.8361513036237369 11103 22-23 0.23146460059366802 11103 24-25 0.2996035700480064 11103 26-27 -0.6009607149927731 11103 28-29 0.33763079879241786 11103 30-31 -0.46061833573307354 11103 32-33 0.06673946062229419 11103 34-35 -0.7167938069588686 11103 36-37 -0.416466547568362 11103 38-39 -1.598813277845263 11103 40-41 -0.3879319907591139 11103 42-43 -1.1998532976809528 11103 44-45 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24-25 0.16485179699835584 11104 26-27 -0.3300387291771685 11104 28-29 0.7012241794070704 11104 30-31 -0.8395781466077707 11104 32-33 -0.3197853620727358 11104 34-35 0.8387617485966885 11104 36-37 -1.2708400700269955 11104 38-39 0.6612340034786115 11104 40-41 0.3503658815813182 11104 42-43 -0.41025518893390966 11104 44-45 -0.019493402400598825 11104 46-47 -0.2157621060482242 11104 48-49 0.3052070426112934 11104 50-51 0.805889821625172 11104 52-53 -1.006875606720314 11104 54-55 -1.1310866227845011 11104 56-57 0.17703246434120246 11104 58-59 -1.1481836194328636 11104 60-61 0.04188727449056984 11104 62-63 0.13664438982327454 11104 64-65 0.846182705801354 11104 66-67 -1.733968142762194 11104 68-69 1.1192826499923498 11104 70-71 -0.4755180768980658 11104 72-73 0.3255621337887291 11104 74-75 0.7063473052832023 11104 76 -1.2406647917093707 11105 1 0.8053520498310576 11105 2 0.23184802302192864 11105 3 -1.8182230565557838 11105 4 -0.12271916712654019 11105 5 -0.8739307563072387 11105 6 1.386733629814195 11105 7 -0.22885612474716055 11105 8 2.011260220570037 11105 9 -0.7063462425094897 11105 10-11 0.18226933298608827 11105 12-13 -0.1332843231746068 11105 14-15 0.32591032488799954 11105 16-17 -0.5557669946009867 11105 18-19 -1.358568683946757 11105 20-21 0.14428347898495986 11105 22-23 0.5433003493859445 11105 24-25 -0.5409761400969231 11105 26-27 -0.5821201352826293 11105 28-29 -0.10705519154574716 11105 30-31 -0.31182606520167155 11105 32-33 0.4725365620715749 11105 34-35 -1.0634121644467882 11105 36-37 -1.450766064476582 11105 38-39 -0.8355282536906756 11105 40-41 -1.483584164672152 11105 42-43 1.366571823091995 11105 44-45 -0.6073749935199331 11105 46-47 -1.0386113197392461 11105 48-49 -0.4792517918012891 11105 50-51 0.01726817500167499 11105 52-53 -1.308216957321605 11105 54-55 -1.139874781896438 11105 56-57 0.1703257113347334 11105 58-59 0.08979047862449363 11105 60-61 -0.2661608291172044 11105 62-63 0.08622810860217811 11105 64-65 -0.2632067484077112 11105 66-67 0.4253750579778597 11105 68-69 0.2517988387064989 11105 70-71 -0.1734829242616165 11105 72-73 0.8128424298109351 11105 74-75 0.713979127669873 11105 76 0.5303805366902594 11106 1 0.4088717185681183 11106 2 -0.14703396455571038 11106 3 -1.5832334085226556 11106 4 0.21993093639312633 11106 5 -1.8646139861209043 11106 6 -0.0904920430636551 11106 7 0.6645186164536696 11106 8 -0.18025116659352136 11106 9 -0.038644379155449826 11106 10-11 -0.25924205417747004 11106 12-13 -0.001296745534858701 11106 14-15 -0.7481062382589911 11106 16-17 0.47218331595802 11106 18-19 -0.09355833197987806 11106 20-21 0.6328963154238849 11106 22-23 0.46876618789525537 11106 24-25 0.13293690338133501 11106 26-27 1.1585630945310328 11106 28-29 -0.17744856628694805 11106 30-31 -0.5209357960505301 11106 32-33 0.4119775558603962 11106 34-35 0.017333177168119107 11106 36-37 0.993850912749096 11106 38-39 0.17896449993251906 11106 40-41 -0.35936056218768186 11106 42-43 -0.81096440879206 11106 44-45 0.18972645575542657 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-0.6865520176538382 21611 38-39 -0.23129191032389684 21611 40-41 2.0710423682152452 21611 42-43 0.6121125142848669 21611 44-45 0.15126491729388647 21611 46-47 1.0070965955338878 21611 48-49 0.4672365025130958 21611 50-51 0.9542804381008949 21611 52-53 -0.2990196329068908 21611 54-55 0.8757327989285315 21611 56-57 1.3957995128954863 21611 58-59 -0.19755623263526445 21611 60-61 1.1992795276275032 21611 62-63 0.6865625567627092 21611 64-65 0.7114309327517034 21611 66-67 -0.768883581932954 21611 68-69 0.010717456320769259 21611 70-71 2.733429004389439 21611 72-73 -1.012408406309472 21611 74-75 -1.5165113962989025 21611 76 0.5476603583179056 21612 1 0.6433955280919292 21612 2 0.48510889258714585 21612 3 1.5393856390963911 21612 4 -0.42054525408306276 21612 5 1.8032431567362366 21612 6 0.9226031950315843 21612 7 0.06570909264414126 21612 8 1.7971297857874262 21612 9 1.7827841922731196 21612 10-11 -0.40686110179652246 21612 12-13 -1.731653888391996 21612 14-15 -1.246915762068511 21612 16-17 0.26325474452944775 21612 18-19 -0.4411773795989262 21612 20-21 1.0263486963762674 21612 22-23 0.5384090450381152 21612 24-25 0.007936903381335014 21612 26-27 -0.609294048326106 21612 28-29 -2.0054247567631407 21612 30-31 -0.616173891288625 21612 32-33 -0.40409387271103725 21612 34-35 -0.07373825140330581 21612 36-37 -0.11924432534614482 21612 38-39 0.7122978332658505 21612 40-41 0.5245680092408875 21612 42-43 0.5432022578746043 21612 44-45 -0.1894402109112434 21612 46-47 -1.0146982762609866 21612 48-49 -0.009686574409982995 21612 50-51 -0.8550144336939809 21612 52-53 0.6298265209392682 21612 54-55 0.16066869636443215 21612 56-57 -1.051956897360924 21612 58-59 -0.9949921300711679 21612 60-61 2.1246000404480156 21612 62-63 0.7932933259934813 21612 64-65 -0.134402400581628 21612 66-67 0.5362446231952518 21612 68-69 0.29853796914128594 21612 70-71 0.11836490182533765 21612 72-73 0.2856685167674513 21612 74-75 -0.694716524504031 21612 76 -1.33374989809235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 11.0 19 20.0 20 71.0 21 130.0 22 289.0 23 441.0 24 634.0 25 859.0 26 1020.0 27 1316.0 28 1550.0 29 2059.0 30 2605.0 31 3365.0 32 4722.0 33 7611.0 34 13158.0 35 19286.0 36 8138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.575946733250106 7.122049818679033 9.53421318589858 60.767790262172284 2 17.74095266404102 9.872928587352307 58.93735602288772 13.448762725718957 3 17.480345386181583 16.1071232184523 52.72638102456641 13.68615037079971 4 15.252574791564491 52.513858546227354 23.526089735015677 8.707476927192474 5 15.530488801700178 22.43821243330807 56.36601423751988 5.665284527471874 6 17.78527479713462 63.34601551585768 12.684659513123087 6.1840501738846125 7 18.58457057083835 17.63639335978718 12.876187079227785 50.90284899014669 8 17.36739637671467 63.628932780477655 6.6461575044213586 12.357513338386315 9 18.031714892921364 14.255353931665848 5.631102590396362 62.08182858501642 10-11 18.144055642662344 37.495634339726394 30.487542077534123 13.872767940077132 12-13 18.357186380727335 10.912954954151619 36.70471264880289 34.02514601631816 14-15 18.464190705485457 8.007490302733068 12.876930164816383 60.65138882696509 16-17 44.66836090180867 30.735506115594397 6.09627416885877 18.499858813738165 18-19 47.78812356486263 15.223416983109036 6.182701810966865 30.805757641061465 20-21 21.792471056816325 8.04984618128316 6.301365791311843 63.85631697058868 22-23 19.17383744259664 34.21983444053086 6.066550745314846 40.539777371557655 24-25 44.6722499238309 34.77621146029175 6.637487088408177 13.914051527469178 26-27 49.26165119614736 37.53353597360227 6.637335850234473 6.567476980015904 28-29 45.00148632580262 14.797859690844234 7.574316290130796 32.626337693222354 30-31 51.0188309082532 9.127119777655574 30.232005112732786 9.622044201358442 32-33 20.95629491457406 57.58206315351402 13.9029882358187 7.558653696093222 34-35 17.610296576477495 45.03488916466645 7.328582362951348 30.026231895904704 36-37 17.316123471101402 34.99412962385007 7.653781562560376 40.03596534248815 38-39 17.856346694012217 14.565220622111255 33.55328666755837 34.02514601631816 40-41 17.43379009006331 7.610052611241566 37.92542282198377 37.03073447671135 42-43 43.92592812769372 7.604850934815563 34.906964301637785 13.562256635852926 44-45 21.342904275714478 33.262740202416516 37.6001307830636 7.794224738805415 46-47 43.850458122478095 11.020948049728396 15.1332753713653 29.995318456428205 48-49 43.84725132640778 6.832671987159481 8.645057737750234 40.675018948682514 50-51 23.287559261075693 7.145511019959279 34.97555248413512 34.591377234829906 52-53 16.84054869439862 8.032755212745405 61.18046576604693 13.946230326809042 54-55 16.397791533219888 7.8136030258670015 41.98910628432152 33.79949915659159 56-57 42.89015708829343 7.037020524023957 15.636750040869707 34.43607234681291 58-59 20.014119045849743 9.769636620346288 56.171509251690566 14.0447350821134 60-61 42.66054364141662 32.39333006375674 17.657942841856524 7.288183452970114 62-63 19.746747021973857 65.85023296598771 6.640459541201911 7.762560470836523 64-65 16.237264708286578 69.82246761836112 6.199885558866587 7.740382114485721 66-67 16.37795860736447 69.85471705123918 6.230446252740311 7.5368780886560405 68-69 17.72482054483035 68.61726633673668 6.136400790643067 7.521512327789914 70-71 40.555382168918214 45.605391829030864 5.917190542006629 7.922035460044288 72-73 76.35689445055435 10.26810926492881 5.837767143239307 7.537229141277532 74-75 79.97800433968433 6.909312486995808 5.66536872454894 7.447314448770919 76 79.98959649253177 6.900497882143123 5.509400312105224 7.600505313219886 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 1.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 2.0 16 3.5 17 4.0 18 6.5 19 17.0 20 21.0 21 17.0 22 23.5 23 42.0 24 51.0 25 48.0 26 62.0 27 104.0 28 131.5 29 131.0 30 150.0 31 175.5 32 214.5 33 247.0 34 292.5 35 409.0 36 552.0 37 624.0 38 673.5 39 756.0 40 861.5 41 1011.0 42 1088.0 43 1149.5 44 1410.0 45 2456.0 46 3303.0 47 6960.5 48 18954.5 49 14600.5 50 1910.0 51 1696.5 52 1163.0 53 806.0 54 769.0 55 701.5 56 639.0 57 551.0 58 458.0 59 448.5 60 424.0 61 388.5 62 368.0 63 333.0 64 275.0 65 232.0 66 213.0 67 214.0 68 186.5 69 135.5 70 104.0 71 96.0 72 114.5 73 92.5 74 47.5 75 43.0 76 34.5 77 26.0 78 17.5 79 9.0 80 5.5 81 5.0 82 8.0 83 8.0 84 4.5 85 1.5 86 1.5 87 1.0 88 1.0 89 3.0 90 3.0 91 1.0 92 1.0 93 4.0 94 8.5 95 17.0 96 24.0 97 42.5 98 135.5 99 3713.5 100 7217.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004458513531588568 2 0.002972342354392379 3 0.0 4 0.0 5 0.0 6 0.0014861711771961895 7 0.0 8 0.0 9 0.0 10-11 7.430855885980947E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0037154279429904733 20-21 0.0 22-23 0.0 24-25 0.0037154279429904733 26-27 0.012632455006167609 28-29 0.010403198240373326 30-31 0.005944684708784758 32-33 0.009660112651775233 34-35 0.0037154279429904733 36-37 0.0 38-39 0.0 40-41 0.0014861711771961895 42-43 0.0014861711771961895 44-45 0.0 46-47 0.002229256765794284 48-49 0.0 50-51 0.0 52-53 0.0 54-55 7.430855885980947E-4 56-57 0.0 58-59 0.002972342354392379 60-61 0.0 62-63 0.0037154279429904733 64-65 0.005201599120186663 66-67 0.006687770297382853 68-69 0.0 70-71 0.0 72-73 0.0014861711771961895 74-75 0.0014861711771961895 76 0.002972342354392379 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 67287.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.547029886902372 #Duplication Level Percentage of deduplicated Percentage of total 1 71.45679899407222 11.823977885772884 2 6.736123585414047 2.2292567657942843 3 4.3290820908927605 2.14900352222569 4 3.538710256870846 2.3422057752611947 5 3.4488952757319917 2.8534486602166838 6 2.7752829171905873 2.755361362521735 7 2.0926890605352972 2.423945190006985 8 1.5088916831327466 1.9974140621516787 9 1.158613256691216 1.7254447367247758 >10 2.649541943596192 6.326630701324179 >50 0.14370396982216635 1.7046383402440295 >100 0.09879647925273935 3.856614204824112 >500 0.017962996227770794 2.0969875310238235 >1k 0.026944494341656185 11.636720317446162 >5k 0.008981498113885397 10.929302837100776 >10k+ 0.008981498113885397 33.149048107361004 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 22305 33.149048107361004 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 7354 10.929302837100776 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC 3208 4.7676371364453765 No Hit TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG 3008 4.470402901006138 No Hit CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 1614 2.39868027999465 No Hit CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 825 1.2260912211868562 No Hit ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT 586 0.870896309836967 RNA PCR Primer, Index 36 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC 453 0.6732355432698739 No Hit CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 390 0.5796067591065139 No Hit CTAAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 320 0.4755747767027806 No Hit CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC 267 0.39680770431138257 No Hit CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT 260 0.3864045060710093 No Hit TCATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG 242 0.35965342488147783 No Hit CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG 173 0.2571076136549408 No Hit CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG 140 0.2080639648074665 No Hit CTCTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC 127 0.18874373950391604 No Hit CAAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 118 0.17536819890915037 No Hit CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT 105 0.15604797360559988 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC 99 0.14713094654242276 No Hit TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT 95 0.141186261833638 No Hit CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC 95 0.141186261833638 No Hit TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC 89 0.13226923477046088 No Hit CTTATACACATCTCCGAGCCCACGAGACGGTAACCCATCTCGTATGCCGT 81 0.12037986535289136 No Hit CATAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 75 0.1114628382897142 No Hit CTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG 73 0.10849049593532183 No Hit CTTATACAAATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT 70 0.10403198240373325 No Hit GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA 69 0.10254581122653708 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008917027063177136 0.0 2 0.0 0.0 0.0 0.008917027063177136 0.0 3 0.0 0.0 0.0 0.008917027063177136 0.0 4 0.0 0.0 0.0 0.008917027063177136 0.0 5 0.0 0.0 0.0 0.041612792961493304 0.0 6 0.0 0.0 0.0 0.041612792961493304 0.0 7 0.0 0.0 0.0 0.041612792961493304 0.0 8 0.0 0.0 0.0 0.05052982002467044 0.0 9 0.0 0.0 0.0 0.05052982002467044 0.0 10 0.0 0.0 0.0 0.05052982002467044 0.0 11 0.0 0.0 0.0 0.05052982002467044 0.0 12 0.0 0.0 0.0 0.05052982002467044 0.0 13 0.0 0.0 0.0 0.05201599120186663 0.0 14 0.0 0.0 0.0 0.06390536061943615 0.0 15 0.0 0.0 0.0 0.06390536061943615 0.0 16 0.0 0.0 0.0 0.08025324356859423 0.0 17 0.0 0.0 0.0 0.08025324356859423 0.0 18 0.0 0.0 0.0 0.09362878416335993 0.0 19 0.0 0.0 0.0 0.10849049593532183 0.0 20 0.0 0.0 0.0 0.11740752299849896 0.0 21 0.0 0.0 0.0 0.12037986535289134 0.0 22 0.0 0.0 0.0 0.15902031595999228 0.0 23 0.0 0.0 0.0 0.19914693774428938 0.0 24 0.0 0.0 0.0 0.19914693774428938 0.0 25 0.0 0.0 0.0 0.2363012171741941 0.0 26 0.0 0.0 0.0 0.24670441541456745 0.0 27 0.0 0.0 0.0 0.2541352713005484 0.0 28 0.0 0.0 0.0 0.2630522983637255 0.0 29 0.0 0.0 0.0 0.29277572190764933 0.0 30 0.0 0.0 0.0 0.35816725370428165 0.0 31 0.0 0.0 0.0 0.3745151366534397 0.0 32 0.0 0.0 0.0 0.47408860552558446 0.0 33 0.0 0.0 0.0 0.5290769390818435 0.0 34 0.0 0.0 0.0 0.619733380890811 0.0 35 0.0 0.0 0.0 0.6598600026751081 0.0 36 0.0 0.0 0.0 0.8426590574702394 0.0 37 0.0 0.0 0.0 1.2691901853255458 0.0 38 0.0 0.0 0.0 1.4653647807154428 0.0 39 0.0 0.0 0.0 1.8606863138496292 0.0 40 0.0 0.0 0.0 2.1386003239853166 0.0 41 0.0 0.0 0.0 2.510143118284364 0.0 42 0.0 0.0 0.0 2.909923164950139 0.0 43 0.0 0.0 0.0 3.3156478963246987 0.0 44 0.0 0.0 0.0 3.764471591837948 0.0 45 0.0 0.0 0.0 4.219239972059982 0.0 46 0.0 0.0 0.0 4.635367901674915 0.0 47 0.0 0.0 0.0 5.00393835361957 0.0 48 0.0 0.0 0.0 5.446817364424034 0.0 49 0.0 0.0 0.0 5.802012275773923 0.0 50 0.0 0.0 0.0 6.1230252500483004 0.0 51 0.0 0.0 0.0 6.499026557878937 0.0 52 0.0 0.0 0.0 6.803691649204155 0.0 53 0.0 0.0 0.0 7.176720614680399 0.0 54 0.0 0.0 0.0 7.41450800303179 0.0 55 0.0 0.0 0.0 7.624058139016452 0.0 56 0.0 0.0 0.0 7.9004859779749435 0.0 57 0.0 0.0 0.0 8.138273366326333 0.0 58 0.0 0.0 0.0 8.396867151158471 0.0 59 0.0 0.0 0.0 8.612361971851918 0.0 60 0.0 0.0 0.0 8.859066387266486 0.0 61 0.0 0.0 0.0 9.132521883870584 0.0 62 0.0 0.0 0.0 9.365850758690385 0.0 63 0.0 0.0 0.0 9.643764768826074 0.0 64 0.0 0.0 0.0 9.836967021861579 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCAAG 15 0.002212229 70.02828 18 CTTTACA 15 0.002212229 70.02828 1 AATTTTG 15 0.002212229 70.02828 16 AGCTAGA 15 0.002212229 70.02828 55 GACGGAA 30 1.006847E-7 70.02828 26 AGTGGAT 15 0.002212229 70.02828 26 TGCTGAT 15 0.002212229 70.02828 30 CTGATGA 15 0.002212229 70.02828 32 CGGAAAA 30 1.006847E-7 70.02828 28 GCTGATG 15 0.002212229 70.02828 31 TCGTCAA 15 0.002212229 70.02828 17 TTTGTAA 15 0.002212229 70.02828 19 TTGCTGA 15 0.002212229 70.02828 29 ACTGCTT 20 7.858773E-5 70.028275 53 GAAAACC 25 2.8052364E-6 70.028275 30 GTACACC 20 7.858773E-5 70.028275 30 ACCGAGC 25 2.8052364E-6 70.028275 13 CATTATA 20 7.858773E-5 70.028275 2 AAAAGCG 20 7.858773E-5 70.028275 67 TACTGCT 25 2.8052364E-6 70.028275 52 >>END_MODULE