FastQCFastQC Report
Wed 25 May 2016
SRR1779987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78661
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4397355.9019081883017No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG45175.742362797320146No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT5760.7322561370946212No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG5230.6648784022577897No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC4430.563176160994648No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1670.21230342863680857No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1180.15001080586313423No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1120.14238313776839856No Hit
CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG840.10678735332629893No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTCA150.002217185270.031
CTTTACA150.002217185270.01
AAGGCTC150.002217185270.020
AGTTGAA150.002217185270.021
ACCTCGC150.002217185270.08
AAAAAGG4750.058.94737270
CTGCCGG252.3773199E-456.00000418
ACCACCG200.006928103552.535
TCTGGCG200.006928103552.511
CCACCGA200.006928103552.536
TATTCAA200.006928103552.567
CAGAAGT200.006928103552.517
TTTACAC200.006928103552.52
GCTTTAA200.006928103552.51
GACCACC200.006928103552.534
TCATAGG200.006928103552.52
TGCGGGT200.006928103552.55
TGGCCAT200.006928103552.540
GAAGGGC200.006928103552.56
TCGCCGT7350.051.90476652
Produced by FastQC (version 0.11.5)