FastQCFastQC Report
Wed 25 May 2016
SRR1779987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78661
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT2519732.03239216384231No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75899.647728861824792No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG34614.399893212646674No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC31874.051563036320413No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT18322.328981324925948No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT9611.2216981731734913RNA PCR Primer, Index 36 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT9221.1721183305577096No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT5120.6508943440841077No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC4690.596229389405169No Hit
CTAAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT4000.5085112063157091No Hit
TCATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG3190.40553768703677806No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC3110.3953674629104639No Hit
CAAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT1770.2250162087947013No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT1770.2250162087947013RNA PCR Primer, Index 36 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG1370.1741650881631304No Hit
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC1300.16526614205260548No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC1280.16272358602102693No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGACGT1250.1589097519736591No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC1200.15255336189471277RNA PCR Primer, Index 36 (95% over 21bp)
CATAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT1160.14746824983155568No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAAAACAATCTCGTATGCCGT1070.1360267476894522No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACACTCTCGTATGCC1000.12712780157892728No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC950.12077141149998093No Hit
CTTATACAAATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT860.10932990935787748No Hit
ATACCCATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT860.10932990935787748RNA PCR Primer, Index 36 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC790.10043096324735257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGG207.851979E-570.0553869
GAATGCC353.6488927E-970.0108242
CGAATGC401.3278623E-1070.0108241
ATCACGT353.6488927E-970.0108237
TATACTC150.00221582270.0108151
AATCACG301.0108306E-770.0108136
GCCTAAC150.00221582270.0108126
ATCTAGT150.00221582270.0108137
TCGAATG550.070.0108140
GTATTCT252.8138456E-670.0108149
TACACAC150.00221582270.010813
CATTATA150.00221582270.010812
TCACGTA301.0108306E-770.0108138
TCTCGAA550.070.0108138
CGAATGA150.00221582270.0108141
CTTAATA252.8138456E-670.010812
TCATTAT150.00221582270.010811
TGAACAA150.00221582270.010815
ATCTGCA150.00221582270.0108152
AATGACG150.00221582270.0108143
Produced by FastQC (version 0.11.5)