FastQCFastQC Report
Wed 25 May 2016
SRR1779986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60060
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3957565.8924408924409No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG37616.262071262071261No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT4150.690975690975691No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3780.6293706293706294No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3370.5611055611055611No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1350.2247752247752248No Hit
CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG720.11988011988011987No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG700.11655011655011654No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATA150.002215353869.9941743
GTGTCGG150.002215353869.9941739
CGGAGGT150.002215353869.9941746
TCGGAGG150.002215353869.9941745
TCAACAA150.002215353869.9941741
TCTCGGA150.002215353869.9941743
CCTTTGT150.002215353869.9941734
CTCGGAG150.002215353869.9941744
CATATAA150.002215353869.994171
AAAAAGG4500.055.9953470
AAGGGGG452.6480848E-854.4399170
GTATCAT6150.052.92242457
TCATTAA6150.052.92242460
CGTATCA6100.052.7824956
CCGTATC6050.052.64024455
ACGCAGC200.0068996452.53937515
CGCAGCC200.0068996452.53937516
GAGGTCG200.006922433652.4956348
TAAACAT200.006922433652.495635
AATTCTA200.006922433652.4956325