FastQCFastQC Report
Wed 25 May 2016
SRR1779986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60060
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT2213536.85481185481186Illumina PCR Primer Index 1 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG608110.124875124875125No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG31945.318015318015318No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC22243.7029637029637033No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT13552.256077256077256Illumina PCR Primer Index 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT7871.3103563103563103Illumina PCR Primer Index 1 (95% over 24bp)
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT7441.2387612387612388Illumina PCR Primer Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC5500.9157509157509158No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT4360.7259407259407259TruSeq Adapter, Index 1 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT3900.6493506493506493Illumina PCR Primer Index 1 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC3350.5577755577755578Illumina PCR Primer Index 1 (95% over 22bp)
CTAAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT2390.39793539793539795Illumina PCR Primer Index 1 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG2310.38461538461538464No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT1530.2547452547452548Illumina PCR Primer Index 1 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC1460.2430902430902431Illumina PCR Primer Index 1 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG1270.21145521145521148No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT1240.20646020646020646No Hit
CAAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT1230.20479520479520483Illumina PCR Primer Index 1 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC1120.1864801864801865No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC960.15984015984015984Illumina PCR Primer Index 1 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG960.15984015984015984No Hit
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT900.14985014985014986Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCGT900.14985014985014986No Hit
CTAATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT860.1431901431901432TruSeq Adapter, Index 1 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCG790.13153513153513152TruSeq Adapter, Index 1 (95% over 22bp)
CATAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT730.12154512154512155Illumina PCR Primer Index 1 (95% over 21bp)
ATACCCATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT720.11988011988011987Illumina PCR Primer Index 1 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGACGT720.11988011988011987No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG680.11322011322011322No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCC680.11322011322011322No Hit
CTCTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC670.11155511155511155No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAACACGATCTCGTATGCCGT670.11155511155511155TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACAAATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT610.10156510156510157Illumina PCR Primer Index 1 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG150.00221095770.0291770
CAACACT150.00221095770.0291730
TCTACTG500.070.0291750
AAGGGGC150.00221095770.0291769
CTAATAA150.00221095770.029171
CCTTATA207.851412E-570.029171
AGCATGA150.00221095770.0291755
TCTTTAT950.070.029171
CAAATAC850.070.029171
CAAATAA150.00221095770.029171
TAGACCC150.00221095770.0291724
CGTCTAC500.070.0291748
ATCTCGA401.3278623E-1069.9708637
CAACTCC207.883923E-569.970869
ACCGAGC207.883923E-569.9708613
TCACGTA207.883923E-569.9708638
ATCACGT207.883923E-569.9708637
CTCGAAT550.069.97085639
ACACGTA150.00221828269.97085638
CACGTAT252.8152717E-669.97085639
Produced by FastQC (version 0.11.5)