FastQCFastQC Report
Wed 25 May 2016
SRR1779985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374105
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3971110.614934309886262No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG36780.9831464428435867No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG4780.12777161492094466No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT4290.1146736878683792No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3820.10211037008326539No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC200.0069483952.561
ACTCTCG200.0069483952.528
GCATATG200.0069483952.540
GGCGCCG305.874292E-446.66666429
GCCGTAT6750.046.1481554
TCGCCGT6950.044.31654752
CGGTGGT6950.044.31654746
TGGTCGC6800.044.26470649
TCTCGGT6850.043.94160543
GTCGCCG7050.043.68794351
CGCCGTA7050.043.68794353
CTCGGTG6850.043.43065644
TCGGTGG7100.043.38028345
ATCTCGG7050.043.19148642
AGATCTC7150.043.07692340
GTAGATC7250.042.48275838
GATCTCG7200.042.29166841
CGTATCA7400.042.09459356
AATCCAG503.4745717E-642.0000045
CCGTATC7600.040.52631455