FastQCFastQC Report
Wed 25 May 2016
SRR1779985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374105
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT224646.004731292016947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62231.663436735675813No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC33150.886114860801112No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG31720.847890298178319No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT15430.412451049838949No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT7790.20823030967241818No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT7210.19272664091632027TruSeq Adapter, Index 25 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC4580.12242552224642814No Hit
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT4110.10986220446131433No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG150.002224065169.9973333
CGTGGAC150.002224065169.9973343
CACGTAT150.002224065169.9973339
AAAGGTC150.002224065169.997333
GCCGTAT150.002224065169.9973346
CCTTGAT150.002224065169.997332
AGGGGGG32800.061.3543670
TGCCGTA308.370987E-658.33110445
GATCATA252.3893962E-455.9978641
ATGGCCG200.0069494452.49799311
GTGATAC200.0069494452.49799341
CATTCGA200.0069494452.49799311
AGCGGCC200.0069494452.49799362
ACGTATG200.0069494452.49799340
AAGGGGG38950.051.3972769
AGATATC552.2810127E-950.90714333
CGTCTTC39300.050.0489848
GATAACT352.0852223E-549.99809334
AAGCGGA352.0852223E-549.99809345
CGTATGC41650.049.8300341