FastQCFastQC Report
Wed 25 May 2016
SRR1779984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140644
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3018021.45843406046472No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG25841.8372628764824663No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3460.24601120559711045No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3120.2218366940644464No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3020.21472654361366286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACG150.002220834170.026
CATGTAC150.002220834170.024
GCAGCAC150.002220834170.013
TGTGTCG150.002220834170.03
AATCGAT150.002220834170.050
GACGTCC200.00693941752.531
AGGACGG200.00693941752.563
CACGACA200.00693941752.521
CACTGGA200.00693941752.542
TCGATAA200.00693941752.552
CCCGTGT200.00693941752.516
GTAGATT200.00693941752.515
GTCGTTT200.00693941752.56
TTGGTCG200.00693941752.564
AGCTCGT200.00693941752.510
GCTGTAC200.00693941752.542
TGTCGTT200.00693941752.55
TCGGTCA200.00693941752.529
ATCGATA200.00693941752.551
AGAGATA200.00693941752.533
Produced by FastQC (version 0.11.5)