FastQCFastQC Report
Wed 25 May 2016
SRR1779984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140644
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1675811.915190125423054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44703.1782372515002417No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC27841.979465885498137No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG20591.4639799778163307No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT12520.890190836438099No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT5960.42376496686669896No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT5770.4102556810102102Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC3540.25169932595773725No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3180.22610278433491654No Hit
CTAAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT2380.1692215807286482No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1940.13793691874520064No Hit
TCATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1680.11945052757316346No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC150.002218640670.01742620
TCGATTA150.002218640670.01742632
ATCGATT150.002218640670.01742631
CGTATCG150.00222177469.9925313
CTCCATG150.00222177469.9925310
ACTACGT150.00222177469.992533
CGCAGAT150.00222177469.9925336
GTATCGG150.00222177469.9925314
ACCGTAT150.00222177469.9925311
AGGGGGG23050.064.2230870
ACGATAC352.9447256E-760.036327
CCGACTT352.9592593E-759.99360347
TGCCGAC601.8189894E-1258.3271145
GGACACG252.3766831E-456.0338821
TCGGTTT252.3766831E-456.0338826
TCTGGAT252.3766831E-456.0338823
TTATCGA252.3808678E-456.01394329
GTTGATC252.3850583E-455.9940317
GCCGACT452.6830094E-854.43863746
CAAATAC850.053.52371
Produced by FastQC (version 0.11.5)