FastQCFastQC Report
Wed 25 May 2016
SRR1779983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278502
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3420412.281419882083432No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG29061.0434395444197888No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG4060.14577992258583422No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3250.11669575083841409No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3180.11418230389727901No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTC150.00222315369.9998221
ACGTCCC150.00222315369.9998220
ACGGTGG150.00222315369.9998245
ACGCGCG150.00222315369.999829
GTTTGAC200.006946606652.49986328
TTAGACC200.006946606652.49986360
ATGGTCG200.006946606652.4998638
GGTCTTC200.006946606652.4998631
CGTCCGT200.006946606652.49986320
AGACTCG305.8717665E-446.66654231
GCCGTAT4400.044.54533854
TCGCCGT4500.044.33321852
GTCGCCG4550.043.84604351
TTCGCTC404.5858702E-543.74988632
TGGTCGC4600.042.60858549
CGCCGTA4700.042.446753
CTCGGTG4600.041.84771744
TCGGTGG4600.041.84771745
ATCTCGG4650.041.39774742
CGGTGGT4750.041.2630546
Produced by FastQC (version 0.11.5)