FastQCFastQC Report
Wed 25 May 2016
SRR1779983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278502
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT178946.425088509238713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50921.8283531177513985No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC27740.9960431163869559No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG21530.7730644663234016No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT9970.35798665718738104No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT4330.15547464650164092No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT4210.15116588031683795Illumina PCR Primer Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC3660.13141736863649095No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT2900.10412851613273873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT252.8353406E-670.0000139
AACTCGT455.456968E-1270.0000137
AACGTAT150.002223129870.039
TCCGCAC150.002223129870.036
TGACGAC150.002223129870.045
TTGTTCT150.002223129870.049
ATACGAT150.002223129870.037
CAACTCG650.064.6153936
AGGGGGG24300.061.64609570
ACGTATG1200.061.2540
ATATCGT352.9677358E-760.037
CACGGTC252.3878817E-456.00000430
ACGTTAG252.3878817E-456.00000470
CGTCTAC252.3878817E-456.00000448
CGATCCT252.3878817E-456.00000470
CGAATGC452.6937414E-854.44444741
CGTATGA653.6379788E-1253.84615341
TCGCATT200.006946536252.527
TTAGCCG200.006946536252.553
AAAAGCG200.006946536252.568