FastQCFastQC Report
Wed 25 May 2016
SRR1779982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133923
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2604019.444008870769025No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG66164.94015217699723No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG9260.691442097324582No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC9040.6750147472801535No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT8260.6167723243953616No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2770.20683527101394086No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1670.12469852079179827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTGC150.002217970670.0209217
ATGTACC150.0022212669.99477461
ACCGTCC150.0022212669.9947741
TGGTGCC150.0022212669.99477468
GATATTA252.3842686E-455.9958231
ACTAAGT252.3842686E-455.9958238
GCATCGT200.006930499352.51568615
CATTGTG200.00694074852.4960856
GGCCCCC200.00694074852.496081
TACATGC200.00694074852.496082
TCTAATG200.00694074852.4960842
AAACAGT200.00694074852.4960834
CGCCGAA200.00694074852.4960853
CCGCACA200.00694074852.4960834
AATGTAG200.00694074852.4960825
CTAAGTT352.0784324E-549.9962659
TACGGGG305.852078E-446.6806130
ACGGGGG305.852078E-446.6806131
GAGATGA305.852078E-446.6806116
CGGGGAA305.862868E-446.663188
Produced by FastQC (version 0.11.5)