FastQCFastQC Report
Wed 25 May 2016
SRR1779982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133923
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT1391610.391045600830328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113958.508620625284678No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT21481.6039067225196566No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG11810.8818500182940944No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC8450.6309595812519134No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT6000.4480186375753231TruSeq Adapter, Index 11 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT5020.374842260104687No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC3470.2591041120643952No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGA2770.20683527101394086No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT2390.17846075730083705No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT1970.14709945267056443No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAA207.883886E-570.0343825
GCGTGGG150.002216278370.0343826
GACTAAG207.883886E-570.0343826
CGTGGTC150.002219566670.008224
AAAAGCG150.002219566670.0082268
CTGAGCA150.002222858469.982089
GGGACGG150.002222858469.9820837
CGAATGC500.069.9820841
TCGTATT150.002222858469.9820840
TCCGTCT150.002222858469.9820846
CAGTATT150.002222858469.9820847
TGCAGAT150.002222858469.9820833
ATTATAC18900.063.8963851
TCGAATG550.063.62006840
CGTCTAC501.4551915E-1163.00739748
AGGGGGG15550.061.00394470
TGCCGTA352.961242E-759.9846445
AGCCGTC308.352561E-658.31839845
ACGTATG308.352561E-658.31839840
AATCTCG553.45608E-1157.2580636
Produced by FastQC (version 0.11.5)