FastQCFastQC Report
Wed 25 May 2016
SRR1779981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38930
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2034852.268173645003856No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG532313.673259696891856No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC7792.0010274852298995No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT6051.554071410223478No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG4671.19958900590804No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2280.5856665810428975No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1260.32365784741844333No Hit
TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT960.24659645517595682No Hit
TCTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG780.20035961983046494No Hit
CTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT710.1823786283072181No Hit
CTTTACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG580.14898535833547394No Hit
ACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGC420.10788594913948113Illumina Single End PCR Primer 1 (100% over 21bp)
CTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT400.10274852298998202No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCA150.002208736370.000011
TATAAAC150.002208736370.000013
AGGAAAG150.002208736370.0000159
CACCTCA150.002208736370.0000116
ATAAACA150.002208736370.000014
CTTATAA150.002208736370.000011
TCTTTAT453.6925485E-1062.2222181
TTTATAC453.6925485E-1062.2222183
CTTTATA509.404175E-1056.0000042
TCAATAA252.362335E-456.00000460
CAATAAA252.362335E-456.00000461
ATCAATA252.362335E-456.00000459
TTAATAC252.362335E-456.0000043
CGAATCA407.29744E-752.49999656
TTGGATG200.006901905452.49999652
GAAAGTT200.006901905452.49999661
AAGGGGG352.0501571E-550.070
GCCGAAT352.0501571E-550.054
CGCCGAA352.0501571E-550.053
CCGAATC352.0501571E-550.055
Produced by FastQC (version 0.11.5)