FastQCFastQC Report
Wed 25 May 2016
SRR1779981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38930
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT1174330.164397636783974No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG912723.44464423323915No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT15103.878756742871821No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG9292.3863344464423326No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT6601.6953506293347034TruSeq Adapter, Index 9 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC5861.5052658618032366No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGACGT3110.7988697662471101No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC2960.7603390701258669No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGA1570.4032879527356794No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAGT1310.33650141279219115No Hit
ATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC1150.2954020035961983No Hit
ATTATACACATCACCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT980.251733881325456No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT940.24145902902645774No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC900.23118417672745953No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC870.2234780375032109No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATATCGTATGCCGT800.20549704597996404No Hit
ATTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT790.20292833290521448No Hit
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT700.17980991523246853No Hit
ATTATACACATCTCCGAGCCCACGAGACGAATTCGGATCTCGTATGCCGT640.16439763678397123No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC630.16182892370922167No Hit
TATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG550.14127921911122526No Hit
AATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT530.13614179296172618No Hit
ATTATACACATCTCCGAGCCAACGAGACGTATTCGGATCTCGTATGCCGT520.13357307988697661No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC520.13357307988697661No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATACGGATCTCGTATGCCGT460.11816080143847933No Hit
ATTATACACATCTCCGAGACCACGAGACGTATTCGGATCTCGTATGCCGT410.10531723606473156No Hit
ATTATAAACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT410.10531723606473156No Hit
ATTATACACAACTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT410.10531723606473156No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT400.10274852298998202No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTCG207.7879624E-570.08108529
GTAATCG353.5561243E-970.08108529
CGAATTC252.7705173E-670.0810828
AGAGACG150.002198597670.0810823
GACGAAT309.904943E-870.0810826
ACTCGTA207.837858E-569.99100538
TGATTGA353.592504E-969.99100555
CTGATTG207.837858E-569.99100554
TCGGAAC252.7918304E-669.99100533
GATTGAA353.592504E-969.99100556
AACTCGT207.837858E-569.99100537
CGGAACT252.7918304E-669.99100534
TCTCGAA207.837858E-569.99100538
GAACTCG207.837858E-569.99100536
ATCGGAT353.592504E-969.99100532
ATTGAAA401.2914825E-1069.99100557
GGAACTC252.7918304E-669.99100535
AATCGGA401.2914825E-1069.99100531
CTTCTGA207.837858E-569.99100551
TTCGGAA207.837858E-569.99100532