FastQCFastQC Report
Wed 25 May 2016
SRR1779980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63637
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3597556.53157754136744No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG821612.910728035576788No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC10081.5839841601583984No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT9451.4849851501484985No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG7931.2461303958388987No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG3140.4934236371921995No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1860.2922827914577997No Hit
TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT1540.24199758002419977No Hit
CTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT1050.16499835001649985No Hit
CTTTACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG930.14614139572889984No Hit
TCTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG900.14142715715699986No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCAAGTGTAGATCTCGGTG730.11471313858289987No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG660.1037132485817999No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGA207.8712605E-570.0000149
TCGACGT207.8712605E-570.0000152
TCTGGCG207.8712605E-570.0000111
ACGAGAG207.8712605E-570.0000124
AGAGATC207.8712605E-570.0000127
GACGAGA207.8712605E-570.0000123
GAGAGAT207.8712605E-570.0000126
TCTTTAT207.8712605E-570.000011
TGTGCGC207.8712605E-570.000013
GAGATCT207.8712605E-570.0000128
CCGGCGG207.8712605E-570.0000121
GTAGATA150.002215226970.038
ACAGAGT150.002215226970.049
CTTTACA150.002215226970.01
TGCGCGT150.002215226970.05
TTGTACG150.002215226970.02
GACAGAG150.002215226970.048
TGCCGGC252.810235E-670.019
ACGTATC150.002215226970.055
GCGCGTC150.002215226970.06
Produced by FastQC (version 0.11.5)