##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779980_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63637 Sequences flagged as poor quality 0 Sequence length 76 %GC 79 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.95056335150934 32.0 27.0 32.0 22.0 32.0 2 29.517261970237442 32.0 32.0 32.0 22.0 32.0 3 29.331913823718907 32.0 32.0 32.0 22.0 32.0 4 28.612631016546977 32.0 27.0 32.0 13.0 32.0 5 29.52857614281 32.0 32.0 32.0 22.0 32.0 6 33.93935917783679 37.0 37.0 37.0 27.0 37.0 7 33.58937410625894 37.0 37.0 37.0 22.0 37.0 8 33.79760202397976 37.0 37.0 37.0 27.0 37.0 9 34.03787104986093 37.0 37.0 37.0 27.0 37.0 10-11 33.739962600374 37.0 37.0 37.0 22.0 37.0 12-13 33.45267690180241 37.0 37.0 37.0 22.0 37.0 14-15 33.493840061599386 37.0 34.5 37.0 20.0 37.0 16-17 34.15759699545862 37.0 37.0 37.0 24.5 37.0 18-19 33.96815531844682 37.0 37.0 37.0 24.5 37.0 20-21 34.20579222779201 37.0 37.0 37.0 24.5 37.0 22-23 34.32560460109685 37.0 37.0 37.0 29.5 37.0 24-25 33.477041658154846 37.0 34.5 37.0 17.5 37.0 26-27 33.76061096531892 37.0 34.5 37.0 20.0 37.0 28-29 34.04313528293288 37.0 37.0 37.0 24.5 37.0 30-31 33.91499442148435 37.0 37.0 37.0 22.0 37.0 32-33 34.20629507990634 37.0 37.0 37.0 27.0 37.0 34-35 33.72772129421563 37.0 34.5 37.0 24.5 37.0 36-37 34.687485268004465 37.0 37.0 37.0 29.5 37.0 38-39 34.91902509546333 37.0 37.0 37.0 32.0 37.0 40-41 34.468713170011156 37.0 37.0 37.0 29.5 37.0 42-43 34.34908936624919 37.0 37.0 37.0 27.0 37.0 44-45 34.21437999905715 37.0 37.0 37.0 27.0 37.0 46-47 34.34119301664126 37.0 37.0 37.0 29.5 37.0 48-49 34.676823231767685 37.0 37.0 37.0 29.5 37.0 50-51 34.93194210915034 37.0 37.0 37.0 32.0 37.0 52-53 34.350762920942216 37.0 37.0 37.0 27.0 37.0 54-55 34.363098511872025 37.0 37.0 37.0 29.5 37.0 56-57 34.247340383739015 37.0 37.0 37.0 27.0 37.0 58-59 33.93290067099329 37.0 37.0 37.0 24.5 37.0 60-61 33.91847510096328 37.0 37.0 37.0 22.0 37.0 62-63 33.31865895626758 37.0 34.5 37.0 22.0 37.0 64-65 32.99756431007118 37.0 34.5 37.0 17.5 37.0 66-67 33.41817653252039 37.0 37.0 37.0 22.0 37.0 68-69 33.68911168031177 37.0 37.0 37.0 22.0 37.0 70-71 33.65824127473011 37.0 37.0 37.0 22.0 37.0 72-73 33.52519760516681 37.0 37.0 37.0 22.0 37.0 74-75 32.3124204472241 37.0 32.0 37.0 17.5 37.0 76 31.374766252337476 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.21137086483215128 11101 2 0.7605371689872733 11101 3 0.6574938976889193 11101 4 -2.49831634821631 11101 5 -0.35235227564574245 11101 6 -0.9715907804249326 11101 7 -0.2857726596433494 11101 8 -0.24757057389806647 11101 9 -1.129419811095218 11101 10-11 -0.3697081958491708 11101 12-13 -0.2651247302326212 11101 14-15 -2.751095153888791 11101 16-17 0.018788810867839345 11101 18-19 1.4663950786072846 11101 20-21 -0.12113246868825911 11101 22-23 -1.3013460961651617 11101 24-25 -1.3559307270787144 11101 26-27 -2.1563291146067662 11101 28-29 0.8004798789145013 11101 30-31 -0.5626249875480767 11101 32-33 0.4192512499828922 11101 34-35 -1.3941714605798836 11101 36-37 -1.0965307070832253 11101 38-39 -1.9589661569673282 11101 40-41 -2.50655512019722 11101 42-43 -3.1105073719679233 11101 44-45 -1.9349010499246688 11101 46-47 -2.6714520935117534 11101 48-49 -1.3387239269530227 11101 50-51 -1.0072973004357664 11101 52-53 -3.0461874832061397 11101 54-55 -0.7940670536719949 11101 56-57 -3.700744489864725 11101 58-59 -1.2815365636271139 11101 60-61 -2.5966193873872 11101 62-63 -2.5657909615424757 11101 64-65 -3.8424191326435064 11101 66-67 -2.1845846988158826 11101 68-69 -1.3719565492559411 11101 70-71 -0.5351194511651727 11101 72-73 -2.8391974677676934 11101 74-75 -3.0092075217727015 11101 76 1.122507238894002 11102 1 0.5100577065964202 11102 2 0.5652990737491805 11102 3 0.7503510405460609 11102 4 0.9254931755932141 11102 5 1.2643143910209247 11102 6 1.3950758862417345 11102 7 1.9261321022614126 11102 8 1.785762759435265 11102 9 -0.35084838252378603 11102 10-11 1.4005298993889284 11102 12-13 0.7836847935769029 11102 14-15 0.4643810365873975 11102 16-17 0.20450309658212262 11102 18-19 1.249728411940616 11102 20-21 0.7872008646450723 11102 22-23 1.0641300943110323 11102 24-25 0.8357359395879485 11102 26-27 0.22581374253609 11102 28-29 -0.09356774013311764 11102 30-31 0.09689882197572786 11102 32-33 0.3430607737924163 11102 34-35 -0.2167905081989332 11102 36-37 1.1629931024405806 11102 38-39 1.0457957477945783 11102 40-41 0.29939726075516404 11102 42-43 0.838302151841603 11102 44-45 1.9341465691229445 11102 46-47 1.1654526683930087 11102 48-49 0.5874665492374476 11102 50-51 1.217702699564235 11102 52-53 -0.08666367368233097 11102 54-55 1.638075803470862 11102 56-57 0.8659221768019378 11102 58-59 1.1434634363728833 11102 60-61 1.1307615649937546 11102 62-63 -0.22650524725676036 11102 64-65 1.0349618197374468 11102 66-67 1.4666057773745962 11102 68-69 0.8923291650297713 11102 70-71 1.337499596453867 11102 72-73 -0.5070546106248344 11102 74-75 -0.2270646646298502 11102 76 0.6677453341320998 11103 1 0.9156132621519788 11103 2 0.47085462930473554 11103 3 -0.3940934038983812 11103 4 -0.7800623799623416 11103 5 0.6476477243542575 11103 6 -0.7493685582027112 11103 7 -1.1905345644052545 11103 8 -1.1920150183425093 11103 9 0.5769293952539911 11103 10-11 0.0033076771667026605 11103 12-13 -1.1302040953119814 11103 14-15 -0.3522856300792654 11103 16-17 0.4850586521376812 11103 18-19 0.29972841194061317 11103 20-21 0.23997864242285516 11103 22-23 0.5002412054221423 11103 24-25 0.685735939587957 11103 26-27 0.47859152031387 11103 28-29 -0.0824566290220119 11103 30-31 -0.6142122891353807 11103 32-33 -0.5986058928742466 11103 34-35 -0.24734606375448465 11103 36-37 1.143548657996135 11103 38-39 0.2930179700167983 11103 40-41 0.08273059408849548 11103 42-43 0.17719104073049152 11103 44-45 0.4897021246785016 11103 46-47 0.9237860017263415 11103 48-49 0.3457998825707804 11103 50-51 1.5760360328975693 11103 52-53 -0.16721922923787957 11103 54-55 0.9380758034708592 11103 56-57 0.42425551013527496 11103 58-59 0.3295745474840004 11103 60-61 0.3696504538826417 11103 62-63 0.9957169749654611 11103 64-65 0.9988507086263354 11103 66-67 -0.08339422262540097 11103 68-69 1.286773609474217 11103 70-71 -1.7513892924350216 11103 72-73 1.3512787227084928 11103 74-75 1.889602002036824 11103 76 1.912189778576547 11104 1 -0.6649422934035769 11104 2 0.6069657404158448 11104 3 -0.3496489594539369 11104 4 -1.441173491073453 11104 5 1.5476477243542561 11104 6 0.3284092195750645 11104 7 -0.7572012310719174 11104 8 -0.5475705738980636 11104 9 0.8491516174762097 11104 10-11 -0.578636767277743 11104 12-13 -0.43298187308975855 11104 14-15 0.08104770325406463 11104 16-17 -0.1829969034178731 11104 18-19 0.09139507860728457 11104 20-21 -0.15863246868826053 11104 22-23 0.8307967609776981 11104 24-25 0.25656927292128273 11104 26-27 -0.7991862574639086 11104 28-29 0.15226559320021948 11104 30-31 0.405232155309065 11104 32-33 -0.7361058928742494 11104 34-35 0.10404282513440677 11104 36-37 -1.0536735642260808 11104 38-39 0.17496241446124117 11104 40-41 -0.3547694059115045 11104 42-43 0.5341354851749287 11104 44-45 -0.3241867642103813 11104 46-47 -0.23038066494031995 11104 48-49 0.9041332159041104 11104 50-51 -0.5072973004357664 11104 52-53 -1.1033303403489967 11104 54-55 -0.549424196529138 11104 56-57 0.8492555101352721 11104 58-59 -0.6690365636271167 11104 60-61 -0.22340510167291683 11104 62-63 0.24849475274323396 11104 64-65 -0.9692048469292232 11104 66-67 0.483272444041269 11104 68-69 1.0173291650297713 11104 70-71 -0.5208337368794673 11104 72-73 0.4179453893751699 11104 74-75 0.9104353353701526 11104 76 0.7260786674654334 11105 1 -0.5399422934035769 11105 2 0.10696574041584483 11105 3 0.47535104054606236 11105 4 0.7838265089265484 11105 5 -0.40235227564574316 11105 6 0.3534092195750702 11105 7 -0.6322012310719174 11105 8 -0.17257057389806363 11105 9 0.6991516174762111 11105 10-11 0.02136323272226548 11105 12-13 -0.72048187308976 11105 14-15 1.0935477032540604 11105 16-17 0.5795030965821226 11105 18-19 -1.2836049213927154 11105 20-21 -1.9711324686882605 11105 22-23 -1.6192032390222977 11105 24-25 -0.8434307270787116 11105 26-27 0.5133137425360914 11105 28-29 -1.4727344067997805 11105 30-31 -1.4822678446909379 11105 32-33 -1.698605892874248 11105 34-35 0.029042825134403927 11105 36-37 0.15882643577391065 11105 38-39 -1.3625375855387531 11105 40-41 -0.6547694059115017 11105 42-43 -0.3158645148250656 11105 44-45 -0.19918676421038128 11105 46-47 -0.14288066494032137 11105 48-49 -0.30836678409588103 11105 50-51 -0.6197973004357635 11105 52-53 -1.2158303403489938 11105 54-55 -0.424424196529138 11105 56-57 -0.5632444898647222 11105 58-59 -0.7315365636271167 11105 60-61 0.026594898327090277 11105 62-63 -0.7890052472567604 11105 64-65 -0.7692048469292203 11105 66-67 -0.6042275559587367 11105 68-69 1.0673291650297685 11105 70-71 0.5541662631205355 11105 72-73 -2.144554610624837 11105 74-75 -0.9895646646298459 11105 76 1.6010786674654298 11106 1 -1.0490332024944884 11106 2 -0.9862160777659703 11106 3 -1.267830777635755 11106 4 -1.5729916728916358 11106 5 0.1931022698088043 11106 6 -2.926136234970386 11106 7 -2.311746685617372 11106 8 -2.27029784662534 11106 9 -3.019030200705604 11106 10-11 -2.986591312732287 11106 12-13 -2.1125273276352132 11106 14-15 -2.1553159331095735 11106 16-17 -0.6727696306906026 11106 18-19 -1.374514012301809 11106 20-21 0.0379584404026474 11106 22-23 -1.0805668753859408 11106 24-25 -0.6968398179878079 11106 26-27 -1.0946408029184553 11106 28-29 -1.0761434977088769 11106 30-31 -1.3743132992363911 11106 32-33 -1.8940604383287933 11106 34-35 -1.3698208112292392 11106 36-37 -0.4764008369533599 11106 38-39 -1.023901221902399 11106 40-41 -1.4740875877296915 11106 42-43 -1.7988190602796124 11106 44-45 -3.0014594914831108 11106 46-47 -1.572426119485776 11106 48-49 -0.9004122386413371 11106 50-51 0.21997542683696025 11106 52-53 0.09666965965100616 11106 54-55 -0.6301060147109538 11106 56-57 -0.6893808535010848 11106 58-59 0.7639179818274329 11106 60-61 -1.8790869198547284 11106 62-63 -1.8060507018022136 11106 64-65 -2.1248866651110383 11106 66-67 -1.49627301050419 11106 68-69 -0.15767083497022583 11106 70-71 -1.4003791914249177 11106 72-73 -1.1411455197157423 11106 74-75 -2.1520646646298474 11106 76 -0.7898304234436573 11107 1 -1.1788311822924662 11107 2 0.5097435181936234 11107 3 -1.3163156261206055 11107 4 0.2254931755932148 11107 5 -1.0467967200901853 11107 6 -0.571590780424934 11107 7 -1.0072012310719174 11107 8 -1.0809039072314022 11107 9 0.6269293952539883 11107 10-11 0.23941878827781693 11107 12-13 -1.0774263175342043 11107 14-15 0.5477143699207261 11107 16-17 -1.4177191256400974 11107 18-19 -0.8780493658371569 11107 20-21 -2.0461324686882634 11107 22-23 -0.5747587945778605 11107 24-25 -0.9698196159676016 11107 26-27 -1.0964084796861258 11107 28-29 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1.6842476079201134 11108 9 0.7082425265671191 11108 10-11 1.2861359599949864 11108 12-13 1.523836308728427 11108 14-15 1.2424113396177034 11108 16-17 1.111321278400304 11108 18-19 1.0686678058800112 11108 20-21 1.7425038949481007 11108 22-23 1.1239785791595196 11108 24-25 -0.14002163616962093 11108 26-27 1.428086469808818 11108 28-29 0.9920383204729433 11108 30-31 0.4779594280363355 11108 32-33 0.23093956167120666 11108 34-35 0.25517918877076085 11108 36-37 0.8531446175921005 11108 38-39 1.2601896871885145 11108 40-41 1.241821503179402 11108 42-43 0.8375445760840279 11108 44-45 1.1917223266987094 11108 46-47 1.5753011532414902 11108 48-49 1.3495877613586629 11108 50-51 0.7767936086551401 11108 52-53 0.653487841469186 11108 54-55 1.0289848943799527 11108 56-57 0.924255510135275 11108 58-59 1.7525543454637926 11108 60-61 0.42773126196344435 11108 62-63 1.1598583891068799 11108 64-65 1.556931516707138 11108 66-67 1.9696360804049036 11108 68-69 0.0696018923025008 11108 70-71 1.088257172211442 11108 72-73 1.3361272075569843 11108 74-75 0.41611715355197276 11108 76 1.6192604856472528 11109 1 1.5490820968403227 11109 2 2.210014520903652 11109 3 1.257668113716793 11109 4 1.4563874845363038 11109 5 1.54398918776889 11109 6 2.418653122014092 11109 7 1.2708475494158833 11109 8 0.8621855236629088 11109 9 1.6101272272323115 11109 10-11 1.500936403453963 11109 12-13 1.4548230049590174 11109 14-15 1.6151940447174766 11109 16-17 1.1947469990211488 11109 18-19 1.4054194688511856 11109 20-21 0.7904528971654017 11109 22-23 1.0100650536606253 11109 24-25 1.3117521997505577 11109 26-27 1.130691791316579 11109 28-29 1.438546081005093 11109 30-31 1.7753541065285745 11109 32-33 0.9959063022477039 11109 34-35 1.4616647763539135 11109 36-37 1.0402288747983022 11109 38-39 1.2831941217783154 11109 40-41 1.3754135209177676 11109 42-43 1.3045623144432312 11109 44-45 0.875203479692054 11109 46-47 0.9735827496938256 11109 48-49 1.2065722402943635 11109 50-51 1.2762392849300852 11109 52-53 1.6332550255046598 11109 54-55 1.4197831205440323 11109 56-57 0.5309628272084481 11109 58-59 1.0568780705192324 11109 60-61 1.5884851422295299 11109 62-63 1.5052020698164128 11109 64-65 2.2634171042902906 11109 66-67 1.0832724440412633 11109 68-69 1.964280384541972 11109 70-71 1.3163613850717581 11109 72-73 1.2953844137654116 11109 74-75 1.771715823175029 11109 76 0.8254689113678708 11110 1 -1.4851803886416732 11110 2 -0.977558069107964 11110 3 -1.4496489594539383 11110 4 -1.2126020625020253 11110 5 0.5643143910209254 11110 6 -0.44778125661540713 11110 7 -0.5595821834528678 11110 8 -1.9380467643742563 11110 9 -0.5222769539523568 11110 10-11 -0.9232796244206014 11110 12-13 -0.24726758737546817 11110 14-15 0.5048572270635887 11110 16-17 0.07831262039164244 11110 18-19 0.35925222146442337 11110 20-21 0.05148657893079189 11110 22-23 -0.03348895330801582 11110 24-25 -1.457121203269189 11110 26-27 -0.6146624479400984 11110 28-29 -0.8483296448950242 11110 30-31 -0.9673868923099889 11110 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-0.18566270246323313 21611 50-51 -0.3414809739051492 21611 52-53 0.36197578209998227 21611 54-55 -0.6792711353046528 21611 56-57 -0.6701322449667657 21611 58-59 -0.21265901260670717 21611 60-61 -0.456313264938224 21611 62-63 -1.9407909615424757 21611 64-65 -0.7174191326435064 21611 66-67 -1.3861153110607773 21611 68-69 -1.6576708349702258 21611 70-71 -0.675425573614163 21611 72-73 -3.0356260391962664 21611 74-75 -1.8893095625890304 21611 76 0.1684256062409446 21612 1 -0.38994229340357833 21612 2 -0.38542556393197813 21612 3 0.35469886663301864 21612 4 -0.22813001281258138 21612 5 1.4411259852238238 21612 6 1.1153657413142 21612 7 1.6971465950150346 21612 8 -0.44322274781111304 21612 9 0.9404559653022986 21612 10-11 0.9556023631570412 21612 12-13 0.8800616051711074 21612 14-15 0.002786833688844581 21612 16-17 1.054503096582124 21612 18-19 0.9990037742594566 21612 20-21 0.8951718791378269 21612 22-23 0.9307967609776995 21612 24-25 0.9321127511821601 21612 26-27 0.7279876555795752 21612 28-29 0.411504723634998 21612 30-31 -0.4523765403431099 21612 32-33 0.17856802016923723 21612 34-35 0.07089065122135452 21612 36-37 0.5463264357739135 21612 38-39 0.7428971970699365 21612 40-41 0.3001218984363234 21612 42-43 -0.1392340800424563 21612 44-45 0.20950888796352274 21612 46-47 0.6212497698422865 21612 48-49 0.3476114767736789 21612 50-51 0.9546592213033591 21612 52-53 0.6401479205205689 21612 54-55 0.4054671078186871 21612 56-57 0.9079511623091889 21612 58-59 0.3543330015902768 21612 60-61 0.24887750702274047 21612 62-63 0.33871214404758376 21612 64-65 -0.25779180345096364 21612 66-67 -0.025423208132650643 21612 68-69 -0.20114909583978857 21612 70-71 0.7389488718161914 21612 72-73 1.0559888676360387 21612 74-75 1.13597881363102 21612 76 1.8880351892045653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 7.0 12 14.0 13 29.0 14 75.0 15 121.0 16 207.0 17 347.0 18 410.0 19 404.0 20 496.0 21 451.0 22 508.0 23 622.0 24 709.0 25 785.0 26 894.0 27 964.0 28 1125.0 29 1395.0 30 1561.0 31 2137.0 32 2980.0 33 4528.0 34 7567.0 35 10509.0 36 24791.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 67.63046655247733 5.202947970520295 4.582239891886795 22.584345585115578 2 64.31007118500244 4.337099486147996 24.812609016766977 6.540220312082594 3 64.51435485645143 5.842513003441394 24.198186589562674 5.444945550544494 4 64.54522597271985 19.094537683072474 9.702055440316801 6.65818090389088 5 64.21107217499254 9.59033266810189 23.39048038091048 2.8081147759950973 6 66.55038028788735 23.17084669055252 5.313030360173487 4.965742661386637 7 65.34877508367774 9.51804767666609 5.328660999104295 19.80451624055188 8 66.36076496377893 23.00548423087198 4.942093436208495 5.691657369140595 9 64.71392428932855 6.191366657761994 3.2905385231861968 25.804170529723276 10-11 66.90604522526203 13.828433144239986 12.78580071342144 6.479720917076544 12-13 65.85162719801373 6.006725647029245 14.167072614988136 13.974574539968888 14-15 76.15223847761521 11.413171582569888 5.626443735562645 6.8081462042522425 16-17 75.78531357543568 6.229866272765844 4.267957320426795 13.716862831371687 18-19 67.70118013105582 4.1438157047000965 11.63788362116379 16.51712054308028 20-21 75.55078886165064 4.74102709158338 5.82689043937394 13.881293607392042 22-23 76.04145387117558 3.893961060389396 3.686534563225796 16.37805050520923 24-25 69.54601882552603 13.371937709194338 3.557678708927196 13.524364756352437 26-27 76.39895029621132 12.796800603422536 3.6975344532268966 7.106714647139242 28-29 76.17345255118877 7.328283860018543 4.799880572622845 11.698383016169839 30-31 75.86938416330122 11.803667677608939 5.301161274101545 7.0257868849882925 32-33 68.14510426324308 7.455568301459843 12.873799833430239 11.52552760186684 34-35 67.33739805459088 11.867309898329587 14.762638087904836 6.032653959174694 36-37 75.21881924037902 7.026572591416944 14.209500762135235 3.5451074060687966 38-39 77.08880054056603 4.959378977638796 14.457783993588635 3.494036488206546 40-41 75.35631786539277 12.704872951270488 7.354997878592642 4.583811304744096 42-43 68.43635335067138 13.463971148633252 13.123757591948019 4.975917908747339 44-45 67.60768106604648 4.9758788126404445 14.488426544305986 12.928013577007086 46-47 75.03103540393168 3.8083190596665464 7.415497273598692 13.745148262803086 48-49 78.28307431211402 3.6244637553624464 13.043512422018638 5.048949510504895 50-51 84.59229693417352 3.6056068010748463 7.043858132847243 4.758238131904395 52-53 69.52637616480978 4.051102346119396 12.904442384147588 13.518079104923236 54-55 75.49931643540707 3.877461225387746 6.0232254820308935 14.599996857174286 56-57 74.94225057749424 4.763738076904945 5.307446925530745 14.986564420070083 58-59 67.969640065057 12.697115649037896 13.135543280978684 6.197701004926418 60-61 65.70155727014159 6.892216792117793 15.589201250844633 11.817024686895989 62-63 65.79741345443688 14.671496142181436 13.295724185615288 6.235366217766393 64-65 65.80735898423075 15.556323807877554 14.599325858587445 4.036991349304252 66-67 65.75943051550604 25.407588412310545 4.890275235124497 3.9427058370589205 68-69 65.80919905086662 26.710090041956725 3.5521787639266464 3.9285321432499956 70-71 65.94276914373714 26.53487750836777 3.4673224696324465 4.055030878262646 72-73 67.17868535600358 25.316246837531626 3.6393921775067963 3.8656756289579963 74-75 71.81570325206445 20.763241221625954 3.5074210555263092 3.913634470783277 76 85.46914530854691 6.9456448292659925 3.691248801797696 3.893961060389396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 1.5 12 2.0 13 6.0 14 5.5 15 1.0 16 2.0 17 3.0 18 14.0 19 16.0 20 12.0 21 17.0 22 20.0 23 23.0 24 52.5 25 82.0 26 76.5 27 92.0 28 107.0 29 101.0 30 102.5 31 139.5 32 185.0 33 195.0 34 211.5 35 240.5 36 266.0 37 279.0 38 312.5 39 347.5 40 415.0 41 549.5 42 618.0 43 1281.0 44 4684.5 45 5855.0 46 4285.0 47 2650.5 48 840.5 49 535.5 50 406.0 51 385.0 52 367.0 53 348.0 54 326.0 55 339.5 56 360.0 57 381.0 58 395.0 59 374.0 60 332.0 61 286.0 62 261.0 63 230.5 64 193.5 65 163.5 66 154.0 67 168.0 68 141.5 69 101.0 70 68.5 71 50.0 72 56.0 73 59.0 74 57.5 75 59.0 76 53.5 77 45.5 78 38.5 79 34.0 80 37.0 81 38.5 82 41.0 83 45.0 84 58.0 85 75.0 86 78.0 87 77.0 88 78.0 89 102.0 90 119.0 91 134.0 92 155.0 93 184.5 94 235.5 95 302.5 96 348.0 97 442.5 98 831.5 99 18930.5 100 36735.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0015714128572999982 5 0.0 6 0.0015714128572999982 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0015714128572999982 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 7.857064286499991E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.857064286499991E-4 60-61 0.0 62-63 0.0 64-65 7.857064286499991E-4 66-67 7.857064286499991E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.857064286499991E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 63637.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.489007967063186 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10857409133271 10.40118170246869 2 7.117893755824791 1.920266511620598 3 4.683131407269339 1.895123905903798 4 3.2618825722273996 1.759982400175998 5 2.6211556383970174 1.7678394644624982 6 1.7241379310344827 1.3954146172823987 7 1.0717614165890028 1.011989880101199 8 0.6989748369058714 0.7542781715039992 9 0.39608574091332716 0.48085233433379954 >10 1.0834109972041006 2.5362603516821975 >50 0.12814538676607642 1.1267030186840987 >100 0.046598322460391424 1.1927023586906988 >500 0.023299161230195712 2.731115545987397 >1k 0.011649580615097856 1.5839841601583984 >5k 0.011649580615097856 12.910728035576788 >10k+ 0.011649580615097856 56.53157754136744 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 35975 56.53157754136744 No Hit CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG 8216 12.910728035576788 No Hit ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC 1008 1.5839841601583984 No Hit TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT 945 1.4849851501484985 No Hit CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG 793 1.2461303958388987 No Hit TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG 314 0.4934236371921995 No Hit CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG 186 0.2922827914577997 No Hit TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT 154 0.24199758002419977 No Hit CTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT 105 0.16499835001649985 No Hit CTTTACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG 93 0.14614139572889984 No Hit TCTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG 90 0.14142715715699986 No Hit CTTATACACATCTGACGCTGCCGACGAGCGATCAAGTGTAGATCTCGGTG 73 0.11471313858289987 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 66 0.1037132485817999 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003142825714599997 0.0 2 0.0 0.0 0.0 0.003142825714599997 0.0 3 0.0 0.0 0.0 0.004714238571899995 0.0 4 0.0 0.0 0.0 0.004714238571899995 0.0 5 0.0 0.0 0.0 0.02042836714489998 0.0 6 0.0 0.0 0.0 0.02042836714489998 0.0 7 0.0 0.0 0.0 0.02042836714489998 0.0 8 0.0 0.0 0.0 0.026714018574099975 0.0 9 0.0 0.0 0.0 0.03457108286059996 0.0 10 0.0 0.0 0.0 0.03457108286059996 0.0 11 0.0 0.0 0.0 0.04399956000439995 0.0 12 0.0 0.0 0.0 0.05028521143359995 0.0 13 0.0 0.0 0.0 0.05028521143359995 0.0 14 0.0 0.0 0.0 0.058142275720099945 0.0 15 0.0 0.0 0.0 0.07228499143579993 0.0 16 0.0 0.0 0.0 0.08014205572229992 0.0 17 0.0 0.0 0.0 0.08014205572229992 0.0 18 0.0 0.0 0.0 0.08014205572229992 0.0 19 0.0 0.0 0.0 0.08957053286609991 0.0 20 0.0 0.0 0.0 0.1052846614390999 0.0 21 0.0 0.0 0.0 0.1052846614390999 0.0 22 0.0 0.0 0.0 0.2105693228781998 0.0 23 0.0 0.0 0.0 0.2105693228781998 0.0 24 0.0 0.0 0.0 0.21685497430739978 0.0 25 0.0 0.0 0.0 0.22785486430849977 0.0 26 0.0 0.0 0.0 0.22785486430849977 0.0 27 0.0 0.0 0.0 0.23414051573769976 0.0 28 0.0 0.0 0.0 0.27342583717019975 0.0 29 0.0 0.0 0.0 0.2765686628847997 0.0 30 0.0 0.0 0.0 0.2891399657431997 0.0 31 0.0 0.0 0.0 0.33471093860489964 0.0 32 0.0 0.0 0.0 0.3928532143249996 0.0 33 0.0 0.0 0.0 0.4274242971855996 0.0 34 0.0 0.0 0.0 0.48399516004839954 0.0 35 0.0 0.0 0.0 0.5609943900560994 0.0 36 0.0 0.0 0.0 0.6599934000659994 0.0 37 0.0 0.0 0.0 0.7668494743623993 0.0 38 0.0 0.0 0.0 0.8815626129452991 0.0 39 0.0 0.0 0.0 1.018275531530399 0.0 40 0.0 0.0 0.0 1.1581312758300988 0.0 41 0.0 0.0 0.0 1.3922717915677987 0.0 42 0.0 0.0 0.0 1.5666986187280985 0.0 43 0.0 0.0 0.0 1.7945534830365981 0.0 44 0.0 0.0 0.0 2.009837044486698 0.0 45 0.0 0.0 0.0 2.162264091644798 0.0 46 0.0 0.0 0.0 2.2879771202287977 0.0 47 0.0 0.0 0.0 2.5174033973945975 0.0 48 0.0 0.0 0.0 2.6808303345537974 0.0 49 0.0 0.0 0.0 2.867828464572497 0.0 50 0.0 0.0 0.0 3.109826044596697 0.0 51 0.0 0.0 0.0 3.2701101560412966 0.0 52 0.0 0.0 0.0 3.4555368732026968 0.0 53 0.0 0.0 0.0 3.5891069660731962 0.0 54 0.0 0.0 0.0 3.777676508949196 0.0 55 0.0 0.0 0.0 3.964674638967896 0.0 56 0.0 0.0 0.0 4.128101576127096 0.0 57 0.0 0.0 0.0 4.277385797570596 0.0 58 0.0 0.0 0.0 4.4235271932994955 0.0 59 0.0 0.0 0.0 4.535097506167795 0.0 60 0.0 0.0 0.0 4.638810754749596 0.0 61 0.0 0.0 0.0 4.737809764759495 0.0 62 0.0 0.0 0.0 4.8760940962018955 0.0 63 0.0 0.0 0.0 4.993950060499395 0.0 64 0.0 0.0 0.0 5.119663089083395 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTCGA 20 7.8712605E-5 70.00001 49 TCGACGT 20 7.8712605E-5 70.00001 52 TCTGGCG 20 7.8712605E-5 70.00001 11 ACGAGAG 20 7.8712605E-5 70.00001 24 AGAGATC 20 7.8712605E-5 70.00001 27 GACGAGA 20 7.8712605E-5 70.00001 23 GAGAGAT 20 7.8712605E-5 70.00001 26 TCTTTAT 20 7.8712605E-5 70.00001 1 TGTGCGC 20 7.8712605E-5 70.00001 3 GAGATCT 20 7.8712605E-5 70.00001 28 CCGGCGG 20 7.8712605E-5 70.00001 21 GTAGATA 15 0.0022152269 70.0 38 ACAGAGT 15 0.0022152269 70.0 49 CTTTACA 15 0.0022152269 70.0 1 TGCGCGT 15 0.0022152269 70.0 5 TTGTACG 15 0.0022152269 70.0 2 GACAGAG 15 0.0022152269 70.0 48 TGCCGGC 25 2.810235E-6 70.0 19 ACGTATC 15 0.0022152269 70.0 55 GCGCGTC 15 0.0022152269 70.0 6 >>END_MODULE