FastQCFastQC Report
Wed 25 May 2016
SRR1779980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63637
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT1826428.700284425727173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1424722.387918977953078No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT57138.977481653754891No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG19623.083112026022597No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC11551.8149818501814983No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT9171.4409855901440984TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC4590.7212785015006993No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT3590.5641372157706994No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC2790.43842418718669957No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGA2030.31899681003189967No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCAGT1820.2859971400285997No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATATCGTATGCCGT1790.2812829014566997No Hit
ATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC1540.24199758002419977No Hit
ATTATACACATCACCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT1490.23414051573769976No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC1400.21999780002199976No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC1250.19642660716249982No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAAAGAATCTCGTATGCCGT1150.18071247858949982No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT1130.1775696528748998No Hit
ATTATACACATCTCCGAGCCAACGAGACGGGAATGAATCTCGTATGCCGT1120.1759982400175998No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAAAGAATCTCGTATGCCGT1100.17285541430299983No Hit
TATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG1000.15714128572999986No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC980.15399846001539985No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGCATCTCGTATGCCGT920.14456998287159986No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC900.14142715715699986No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC880.13828433144239988No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAAACTCGTATGCCGT840.13199868001319986No Hit
AATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT840.13199868001319986No Hit
ATTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG810.12728444144129986No Hit
ATTATAAACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT700.10999890001099988No Hit
ATTATACACATCTCCGAGACCACGAGACGGGAATGAATCTCGTATGCCGT670.10528466143909988No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT650.1021418357244999No Hit
ATTATACACAACTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT640.10057042286719989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTA207.8712605E-570.0000153
TTATGCT207.8712605E-570.0000152
GTATGAA207.8712605E-570.0000142
AGAAGGG401.3096724E-1070.0000125
AACGAGC207.8712605E-570.0000113
ATGACGA207.8712605E-570.0000144
TATGAAG207.8712605E-570.0000143
CATCAGC207.8712605E-570.0000151
ATGCTTG207.8712605E-570.0000154
GACGACT207.8712605E-570.0000146
AGACTGG207.8712605E-570.0000125
CGAGAAG401.3096724E-1070.0000123
GCCGACA207.8712605E-570.0000146
GAGACTG207.8712605E-570.0000124
CGACTAC207.8712605E-570.0000148
GAGACGT207.8712605E-570.0000124
TGTGAGC150.002215226970.02
TAAGCCG301.0087933E-770.043
GTGAGCC150.002215226970.03
ACTTCAG150.002215226970.050
Produced by FastQC (version 0.11.5)