FastQCFastQC Report
Wed 25 May 2016
SRR1779979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489145
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG487349.96309887661123No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG82731.6913185251816947No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC11750.24021506915127416No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG11360.2322419732390191No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT8780.17949687720410104No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC207.928026E-569.992943
AGTCGCT150.002225027769.992942
CGACGTA200.00694962152.5000853
GCCGTAT13550.045.2030254
CTCGGTG13800.044.6377544
CGTATCA14150.044.52303756
CGGTGGT14250.044.45620746
TCGGTGG14100.044.4326945
TCGCCGT13850.043.71847552
GTATCAT14350.043.658657
TCTCGGT14050.043.59436843
TGGTCGC14150.043.28628549
GATCTCG14300.043.0769941
TAGATCT14400.043.02089739
ATCTCGG14300.042.83223342
GTCGCCG14200.042.3944351
CGCCGTA14300.042.3427253
AGATCTC14700.042.14291840
GTAGATC14750.042.0000638
GTGGTCG15100.041.72191248