FastQCFastQC Report
Wed 25 May 2016
SRR1779979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489145
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT174003.5572274070061027Illumina PCR Primer Index 6 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT150183.0702552412883706Illumina PCR Primer Index 6 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145152.9674227478559527No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG33780.6905927690153226No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC20580.4207341381389977No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC17690.3616514530456204No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT10430.21322920606364165Illumina PCR Primer Index 6 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC6080.1242985208884891No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTT150.002225091769.992442
TGCCGTA453.8562575E-1062.215545
AGGGGGG40050.061.6101370
ATTATAC20750.060.210361
CACGTAT308.378291E-658.3270339
CAGTCGT252.3909511E-455.9939461
AACTCGT452.701563E-854.4385637
CCTAGCG200.00695263252.4943242
GCCGTAT407.4810123E-752.49432446
TATGCCG49250.052.36997643
CGTATGC49400.052.281841
TCGTATG50350.052.19892540
CCGTCTT47400.052.05133447
CGTCTTC48100.052.02672248
ATGCCGT49650.051.9480644
GCCGTCT47900.051.9463746
CTCGTAT50400.051.9388339
GTATGCC49950.051.9163142
TCTCGTA50950.051.79028338
TGCCGTC49400.051.64421545