FastQCFastQC Report
Wed 25 May 2016
SRR1779978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309062
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4165713.478525344429274No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG86302.792319987575308No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG11720.379211938057736No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC9690.3135293242132646No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT9430.30511677268638654No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT207.9145655E-570.0087419
GGTCGAC207.920912E-569.9974150
TGGTCGA252.8367012E-669.9974149
GATCTTA252.38873E-455.997931
GACCCAT200.006948268552.498066
CAGATCC200.006948268552.498061
CAGTCGT200.006948268552.498061
TAAATTG200.006948268552.4980642
CACGTGT200.006948268552.4980643
CGGTGGT14550.047.38656246
GCCGTAT14250.047.1561554
TCGGTGG14650.047.06310345
CGCCGTA14400.046.66494453
GGTACAT305.873767E-446.664941
GTCGACG305.873767E-446.6649451
TGTGCGC305.873767E-446.664943
TCGCCGT14500.046.5844852
TGGTCGC14450.046.26126549
GTCGCCG14650.046.1075151
ATCTCGG14600.046.02569642
Produced by FastQC (version 0.11.5)