FastQCFastQC Report
Wed 25 May 2016
SRR1779978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309062
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT160035.177925464793471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148354.800007765432179No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT132774.295901793167714No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG30300.9803858125554096No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC17250.558140437840951No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC13430.4345406423306651No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT7550.24428755395357565RNA PCR Primer, Index 37 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC5930.19187088674764285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATA150.0022227770.004691
TCGGGCA150.00222419769.9933749
TGCCGAC301.0224721E-769.9933745
AAGCCGT501.4551915E-1162.9940344
AGGGGGG36700.062.269370
ATTATAC19050.060.8177221
AGCGTCA252.389412E-455.9946943
GCCGACA252.389412E-455.99469446
CGTCTTA900.054.43928548
CCGTCTT42850.053.41384547
GCCGTCT42700.053.355646
CGTCTTC42750.053.1294748
CGTATGC45050.053.05823541
TCTTCTG42050.053.01518650
TATGCCG44900.053.0016643
GTCTTCT42100.052.95222549
CTCGTAT45550.052.93680639
TCGTATG45700.052.91620640
GTATGCC45050.052.90286642
ATCTCGT45850.052.81942437