FastQCFastQC Report
Wed 25 May 2016
SRR1779977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232090
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3683715.871860054289286No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG74903.227196346244991No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG12000.5170408031367142No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC8190.35288034814080743No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT8010.3451247360937567No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2810.12107372140118058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGG150.00222285169.998551
AGACCGC200.00693975652.5101817
CAAGTTC200.006945674352.49886734
GGGCTCG200.006945674352.4988676
GCGCGTC200.006945674352.4988676
AACCCGC200.006945674352.49886731
ACTAGTG200.006945674352.49886732
CCGTAAT200.006945674352.49886737
CGTAATA200.006945674352.49886738
CGCGTCT200.006945674352.4988677
GTGCGTG200.006945674352.49886727
TGCACGT200.006945674352.4988675
GGCTCGC200.006945674352.4988677
ACGAATA200.006945674352.49886721
GTTGTGC305.8702775E-446.6656651
ATTGACA305.8702775E-446.66566545
GTATCAT12900.041.23941857
GCCGTAT12650.041.2244154
CGTATCA12800.041.0147456
TGGTCGC12750.040.9010849